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The VP53 protein encoded by RNA2 of a fabavirus, broad bean wilt virus 2, is essential for viral systemic infection.
Kim, Myung-Hwi; Choi, Boram; Jang, Seok-Yeong; Choi, Ji-Soo; Kim, Sora; Lee, Yubin; Park, Suejin; Kwon, Sun-Jung; Kang, Jin-Ho; Seo, Jang-Kyun.
Afiliación
  • Kim MH; Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea.
  • Choi B; Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea.
  • Jang SY; Department of International Agricultural Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea.
  • Choi JS; Department of International Agricultural Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea.
  • Kim S; Department of International Agricultural Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea.
  • Lee Y; Department of International Agricultural Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea.
  • Park S; Department of Horticulture, Jeonbuk National University, Jeonju, 54896, Republic of Korea.
  • Kwon SJ; Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea.
  • Kang JH; Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea.
  • Seo JK; Department of International Agricultural Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea.
Commun Biol ; 7(1): 462, 2024 Apr 16.
Article en En | MEDLINE | ID: mdl-38627534
ABSTRACT
Plant viruses evolves diverse strategies to overcome the limitations of their genomic capacity and express multiple proteins, despite the constraints imposed by the host translation system. Broad bean wilt virus 2 (BBWV2) is a widespread viral pathogen, causing severe damage to economically important crops. It is hypothesized that BBWV2 RNA2 possesses two alternative in-frame translation initiation codons, resulting in the production of two largely overlapping proteins, VP53 and VP37. In this study, we aim to investigate the expression and function of VP53, an N-terminally 128-amino-acid-extended form of the viral movement protein VP37, during BBWV2 infection. By engineering various recombinant and mutant constructs of BBWV2 RNA2, here we demonstrate that VP53 is indeed expressed during BBWV2 infection. We also provide evidence of the translation of the two overlapping proteins through ribosomal leaky scanning. Furthermore, our study highlights the indispensability of VP53 for successful systemic infection of BBWV2, as its removal results in the loss of virus infectivity. These insights into the translation mechanism and functional role of VP53 during BBWV2 infection significantly contribute to our understanding of the infection mechanisms employed by fabaviruses.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Virus de Plantas / Fabavirus Idioma: En Revista: Commun Biol Año: 2024 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Virus de Plantas / Fabavirus Idioma: En Revista: Commun Biol Año: 2024 Tipo del documento: Article