Ca2+ Overload Decreased Cellular Viability in Magnetic Hyperthermia without a Macroscopic Temperature Rise.
ACS Biomater Sci Eng
; 10(5): 2995-3005, 2024 05 13.
Article
en En
| MEDLINE
| ID: mdl-38654432
ABSTRACT
Magnetic hyperthermia is a crucial medical engineering technique for treating diseases, which usually uses alternating magnetic fields (AMF) to interplay with magnetic substances to generate heat. Recently, it has been found that in some cases, there is no detectable temperature increment after applying an AMF, which caused corresponding effects surprisingly. The mechanisms involved in this phenomenon are not yet fully understood. In this study, we aimed to explore the role of Ca2+ overload in the magnetic hyperthermia effect without a perceptible temperature rise. A cellular system expressing the fusion proteins TRPV1 and ferritin was prepared. The application of an AMF (518 kHz, 16 kA/m) could induce the fusion protein to release a large amount of iron ions, which then participates in the production of massive reactive oxygen radicals (ROS). Both ROS and its induced lipid oxidation enticed the opening of ion channels, causing intracellular Ca2+ overload, which further led to decreased cellular viability. Taken together, Ca2+ overload triggered by elevated ROS and the induced oxidation of lipids contributes to the magnetic hyperthermia effect without a perceptible temperature rise. These findings would be beneficial for expanding the application of temperature-free magnetic hyperthermia, such as in cellular and neural regulation, design of new cancer treatment methods.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Supervivencia Celular
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Calcio
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Especies Reactivas de Oxígeno
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Canales Catiónicos TRPV
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Campos Magnéticos
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Hipertermia Inducida
Límite:
Humans
Idioma:
En
Revista:
ACS Biomater Sci Eng
Año:
2024
Tipo del documento:
Article
País de afiliación:
China