Nucleoside Phosphorylases make N7-xanthosine.
Nat Commun
; 15(1): 3625, 2024 Apr 29.
Article
en En
| MEDLINE
| ID: mdl-38684649
ABSTRACT
Modern, highly evolved nucleoside-processing enzymes are known to exhibit perfect regioselectivity over the glycosylation of purine nucleobases at N9. We herein report an exception to this paradigm. Wild-type nucleoside phosphorylases also furnish N7-xanthosine, a "non-native" ribosylation regioisomer of xanthosine. This unusual nucleoside possesses several atypical physicochemical properties such as redshifted absorption spectra, a high equilibrium constant of phosphorolysis and low acidity. Ultimately, the biosynthesis of this previously unknown natural product illustrates how even highly evolved, essential enzymes from primary metabolism are imperfect catalysts.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Pentosiltransferasa
/
Ribonucleósidos
/
Xantinas
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Alemania