In silico identification and expression analysis of superoxide dismutases in Tenebrio molitor.
Genes Genomics
; 46(7): 733-742, 2024 Jul.
Article
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| MEDLINE
| ID: mdl-38700830
ABSTRACT
BACKGROUND:
Insects encounter various environmental stresses, in response to which they generate reactive oxygen species (ROS). Superoxide dismutase (SOD) is an antioxidant metalloenzyme that scavenges superoxide radicals to prevent oxidative damage.OBJECTIVE:
To investigate expressions of SODs under oxidative stress in Tenebrio molitor.METHODS:
Here, we investigated the transcriptional expression of SODs by pesticide and heavy metals in Tenebrio moltior. First, we searched an RNA-Seq database for T. molitor SOD (TmSOD) genes and identified two SOD isoforms (TmSOD1-iso1 and iso2). We examined their activities under developmental stage, tissue-specific, and various types (pesticide and heavy metal) of oxidative stress by using qPCR.RESULTS:
Our results revealed two novel forms of TmSODs. These TmSODs had a copper/zinc superoxide dismutase domain, active site, Cu2+ binding site, Zn2+ binding site, E-class dimer interface, and P-class dimer interface. TmSODs (TmSOD1-iso1 and iso2) were expressed in diverse developmental phases and tissues. Pesticides and heavy metals caused an upregulation of these TmSODs.CONCLUSION:
Our findings suggest that the two TmSODs have different functions in T. molitor, providing insights into the detoxification ability of T. molitor.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Superóxido Dismutasa
/
Tenebrio
/
Estrés Oxidativo
Límite:
Animals
Idioma:
En
Revista:
Genes Genomics
Año:
2024
Tipo del documento:
Article