Azithromycin resistance in Escherichia coli and Salmonella from food-producing animals and meat in Europe.
J Antimicrob Chemother
; 79(7): 1657-1667, 2024 07 01.
Article
en En
| MEDLINE
| ID: mdl-38775752
ABSTRACT
OBJECTIVES:
To characterize the genetic basis of azithromycin resistance in Escherichia coli and Salmonella collected within the EU harmonized antimicrobial resistance (AMR) surveillance programme in 2014-18 and the Danish AMR surveillance programme in 2016-19.METHODS:
WGS data of 1007 E. coli [165 azithromycin resistant (MICâ>â16â mg/L)] and 269 Salmonella [29 azithromycin resistant (MICâ>â16â mg/L)] were screened for acquired macrolide resistance genes and mutations in rplDV, 23S rRNA and acrB genes using ResFinder v4.0, AMRFinder Plus and custom scripts. Genotype-phenotype concordance was determined for all isolates. Transferability of mef(C)-mph(G)-carrying plasmids was assessed by conjugation experiments.RESULTS:
mph(A), mph(B), mef(B), erm(B) and mef(C)-mph(G) were detected in E. coli and Salmonella, whereas erm(C), erm(42), ere(A) and mph(E)-msr(E) were detected in E. coli only. The presence of macrolide resistance genes, alone or in combination, was concordant with the azithromycin-resistant phenotype in 69% of isolates. Distinct mph(A) operon structures were observed in azithromycin-susceptible (nâ=â50) and -resistant (nâ=â136) isolates. mef(C)-mph(G) were detected in porcine and bovine E. coli and in porcine Salmonella enterica serovar Derby and Salmonella enterica 1,4, [5],12i-, flanked downstream by ISCR2 or TnAs1 and associated with IncIγ and IncFII plasmids.CONCLUSIONS:
Diverse azithromycin resistance genes were detected in E. coli and Salmonella from food-producing animals and meat in Europe. Azithromycin resistance genes mef(C)-mph(G) and erm(42) appear to be emerging primarily in porcine E. coli isolates. The identification of distinct mph(A) operon structures in susceptible and resistant isolates increases the predictive power of WGS-based methods for in silico detection of azithromycin resistance in Enterobacterales.
Texto completo:
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Base de datos:
MEDLINE
Asunto principal:
Salmonella
/
Pruebas de Sensibilidad Microbiana
/
Azitromicina
/
Farmacorresistencia Bacteriana
/
Escherichia coli
/
Carne
/
Antibacterianos
Límite:
Animals
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Antimicrob Chemother
Año:
2024
Tipo del documento:
Article
País de afiliación:
Dinamarca