The GWAS SNP rs80207740 modulates erythrocyte traits via allele-specific binding of IKZF1 and targeting XPO7 gene.
FASEB J
; 38(10): e23666, 2024 May 31.
Article
en En
| MEDLINE
| ID: mdl-38780091
ABSTRACT
Genome-wide association studies have identified many single nucleotide polymorphisms (SNPs) associated with erythrocyte traits. However, the functional variants and their working mechanisms remain largely unknown. Here, we reported that the SNP of rs80207740, which was associated with red blood cell (RBC) volume and hemoglobin content across populations, conferred enhancer activity to XPO7 gene via allele-differentially binding to Ikaros family zinc finger 1 (IKZF1). We showed that the region around rs80207740 was an erythroid-specific enhancer using reporter assays, and that the G-allele further enhanced activity. 3D genome evidence showed that the enhancer interacted with the XPO7 promoter, and eQTL analysis suggested that the G-allele upregulated expression of XPO7. We further showed that the rs80207740-G allele facilitated the binding of transcription factor IKZF1 in EMSA and ChIP analyses. Knockdown of IKZF1 and GATA1 resulted in decreased expression of Xpo7 in both human and mouse erythroid cells. Finally, we constructed Xpo7 knockout mouse by CRISPR/Cas9 and observed anemic phenotype with reduced volume and hemoglobin content of RBC, consistent to the effect of rs80207740 on erythrocyte traits. Overall, our study demonstrated that rs80207740 modulated erythroid indices by regulating IKZF1 binding and Xpo7 expression.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Polimorfismo de Nucleótido Simple
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Alelos
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Eritrocitos
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Factor de Transcripción Ikaros
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Estudio de Asociación del Genoma Completo
Límite:
Animals
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Humans
Idioma:
En
Revista:
FASEB J
Asunto de la revista:
BIOLOGIA
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FISIOLOGIA
Año:
2024
Tipo del documento:
Article