A target-triggered strand displacement-assisted target recycling based on carbon dots-based fluorescent probe and MSNs@PDA nanoparticles for miRNA amplified detection and fluorescence imaging.
Mikrochim Acta
; 191(6): 351, 2024 05 28.
Article
en En
| MEDLINE
| ID: mdl-38806809
ABSTRACT
A target-triggered strand displacement-assisted target recycling based on carbon dots-based fluorescent probe and mesoporous silica nanoparticles@polydopamine (MSNs@PDA) was established to detect miRNA. The surface of MSNs rich in mesopores was coated with a layer of PDA, which can adsorb and quench the fluorescence of single-stranded Fuel DNA with fluorescent carbon dots (CDs) modified at the end through fluorescence resonance energy transfer (FRET). After adding double-stranded DNA-gold nanoparticles (dsDNA-AuNPs) and target let-7a, it will trigger two toehold-mediated strand displacement reactions (TSDR), leading to the recovery of fluorescence and the recycling of target let-7a (excitation wavelength 380 nm; emission wavelength 458 nm). The recovery value of fluorescence is proportional to the logarithm of the target microRNA let-7a concentration, thus realizing the sensitivity amplification detection of disease markers. The MSNs@PDA@Fuel DNA-CDs/dsDNA-AuNPs nanoplatform based on the strategy of "on-off-on" and TSDR cyclic amplification may hold great potential as an effective and safe nanoprobe for accurate fluorescence imaging of diseases related to miRNA with low abundances.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Polímeros
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Carbono
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Dióxido de Silicio
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MicroARNs
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Puntos Cuánticos
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Colorantes Fluorescentes
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Oro
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Indoles
Límite:
Humans
Idioma:
En
Revista:
Mikrochim Acta
Año:
2024
Tipo del documento:
Article