Protocol for the cryopreservation of Cryptosporidium isolates using enteroids.
STAR Protoc
; 5(2): 103101, 2024 Jun 21.
Article
en En
| MEDLINE
| ID: mdl-38824642
ABSTRACT
A major bottleneck in the progress of Cryptosporidium research is the lack of accessible cryopreservation of Cryptosporidium oocysts. Here, we present a protocol for the cryopreservation of Cryptosporidium isolates using enteroids. We describe the steps for the establishment of enteroid cultures and cryopreservation of C. parvum-infected HCT-8 cultures. We then detail procedures for the recovery and propagation of frozen parasites using enteroids. For complete details on the use and execution of this protocol, please refer to Deng et al.1.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Criopreservación
/
Cryptosporidium
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article