Single-cell transcriptome atlas of testes from mice with high-fat diets.
Sci Data
; 11(1): 573, 2024 Jun 04.
Article
en En
| MEDLINE
| ID: mdl-38834587
ABSTRACT
Obesity is accompanied by multiple known health risks and increased morbidity, and obese men display reduced reproductive health. However, the impact of obesity on the testes at the molecular levels remain inadequately explored. This is partially attributed to the lack of monitoring tools for tracking alterations within cell clusters in testes associated with obesity. Here, we utilized single-cell RNA sequencing to analyze over 70,000 cells from testes of obese and lean mice, and to study changes related to obesity in non-spermatogenic cells and spermatogenesis. The Testicular Library encompasses all non-spermatogenic cells and spermatogenic cells spanning from spermatogonia to spermatozoa, which will significantly aid in characterizing alterations in cellular niches and the testicular microenvironment during high-fat diet (HFD)-induced obesity. This comprehensive dataset is indispensable for studying how HFD disrupts cell-cell communication networks within the testis and impacts alterations in the testicular microenvironment that regulate spermatogenesis. Being the inaugural dataset of single-cell RNA-seq in the testes of diet-induced obese (DIO) mice, this holds the potential to offer innovative insights and directions in the realm of single-cell transcriptomics concerning male reproductive injury associated with HFD.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Testículo
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Análisis de la Célula Individual
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Transcriptoma
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Dieta Alta en Grasa
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Obesidad
Límite:
Animals
Idioma:
En
Revista:
Sci Data
Año:
2024
Tipo del documento:
Article
País de afiliación:
China