Vaccine efficacy induced by virus-like particles containing Leishmania donovani surface glycoprotein GP63.
PLoS Negl Trop Dis
; 18(6): e0012229, 2024 Jun.
Article
en En
| MEDLINE
| ID: mdl-38857253
ABSTRACT
Leishmania donovani surface glycoprotein 63 (GP63) is a major virulence factor involved in parasite escape and immune evasion. In this study, we generated virus-like particles (VLPs) expressing L. donovani GP63 using the baculovirus expression system. Mice were intramuscularly immunized with GP63-VLPs and challenged with L. donovani promastigotes. GP63-VLP immunization elicited higher levels of L. donovani antigen-specific serum antibodies and enhanced splenic B cell, germinal center B cell, CD4+, and CD8+ T cell responses compared to unimmunized controls. GP63-VLPs inhibited the influx of pro-inflammatory cytokines IFN-γ and IL-6 in the livers, as well as thwarting the development of splenomegaly in immunized mice. Upon L. donovani challenge infection, a drastic reduction in splenic parasite burden was observed in VLP-immunized mice. These results indicate that GP63-VLPs immunization conferred protection against L. donovani challenge infection by inducing humoral and cellular immunity in mice.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Leishmania donovani
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Vacunas de Partículas Similares a Virus
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Leishmaniasis Visceral
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Ratones Endogámicos BALB C
Límite:
Animals
Idioma:
En
Revista:
PLoS Negl Trop Dis
Asunto de la revista:
MEDICINA TROPICAL
Año:
2024
Tipo del documento:
Article