Papillary renal neoplasm with reverse polarity has low frequency of alterations in chromosomes 7, 17, and Y.
Virchows Arch
; 485(2): 299-306, 2024 Aug.
Article
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| MEDLINE
| ID: mdl-38877360
ABSTRACT
In papillary renal neoplasm with reverse polarity (PRNRP), the status of chromosomal copy number alterations, especially chromosomes 7/17 gain and chromosome Y loss, has remained controversial. In the literatures, there is a discrepancy among the results of chromosomal alteration in PRNRP depending on the analytical methods. Here, we comprehensively analyzed the status of chromosomal abnormalities in PRNRP. Nineteen PRNRP cases were analyzed by fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC), five of which were additionally subjected to array-based comparative genomic hybridization (aCGH) analysis. Fifteen cases of PRCC were used as controls. From the aCGH results, no genome copy number abnormalities were found in the five PRNRP cases. By FISH, numbers of nuclei with abnormal chromosomal signals in PRNRP (centromere 7 gain 11-21% of nuclei, centromere 17 gain 11% of nuclei, centromere Y loss 14-31% of nuclei) were similar to those in non-neoplastic tubular cells (centromere 7 gain 11-15% of nuclei, centromere 17 gain 12-15% of nuclei, centromere Y loss 13-45% of nuclei). c-MET immunohistochemical overexpression, a substitute marker for chromosome 7 trisomy, was observed in 0 of 19 PRNRP cases, consistent with the analyses by aCGH and NGS regarding chromosome 7 gain. Taken together, the frequency of chromosomal alterations in PRNRP is similar to that in non-neoplastic tubular cells, and lower than that in PRCC. Our data suggest that PRNRP has a different tumorigenesis and is a distinct entity from PRCC.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Cromosomas Humanos Par 7
/
Cromosomas Humanos Par 17
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Hibridación Fluorescente in Situ
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Cromosomas Humanos Y
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Neoplasias Renales
Límite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Virchows Arch
Asunto de la revista:
BIOLOGIA MOLECULAR
/
PATOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Japón