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Papillary renal neoplasm with reverse polarity has low frequency of alterations in chromosomes 7, 17, and Y.
Kiyozawa, Daisuke; Iwasaki, Takeshi; Takamatsu, Dai; Kohashi, Kenichi; Miyamoto, Takumi; Fukuchi, Genshiro; Eto, Masatoshi; Yamashita, Michifumi; Oda, Yoshinao.
Afiliación
  • Kiyozawa D; Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
  • Iwasaki T; Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
  • Takamatsu D; Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
  • Kohashi K; Department of Urology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
  • Miyamoto T; Department of Pathology, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan.
  • Fukuchi G; Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
  • Eto M; Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
  • Yamashita M; Department of Urology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
  • Oda Y; Department of Urology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Virchows Arch ; 485(2): 299-306, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38877360
ABSTRACT
In papillary renal neoplasm with reverse polarity (PRNRP), the status of chromosomal copy number alterations, especially chromosomes 7/17 gain and chromosome Y loss, has remained controversial. In the literatures, there is a discrepancy among the results of chromosomal alteration in PRNRP depending on the analytical methods. Here, we comprehensively analyzed the status of chromosomal abnormalities in PRNRP. Nineteen PRNRP cases were analyzed by fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC), five of which were additionally subjected to array-based comparative genomic hybridization (aCGH) analysis. Fifteen cases of PRCC were used as controls. From the aCGH results, no genome copy number abnormalities were found in the five PRNRP cases. By FISH, numbers of nuclei with abnormal chromosomal signals in PRNRP (centromere 7 gain 11-21% of nuclei, centromere 17 gain 11% of nuclei, centromere Y loss 14-31% of nuclei) were similar to those in non-neoplastic tubular cells (centromere 7 gain 11-15% of nuclei, centromere 17 gain 12-15% of nuclei, centromere Y loss 13-45% of nuclei). c-MET immunohistochemical overexpression, a substitute marker for chromosome 7 trisomy, was observed in 0 of 19 PRNRP cases, consistent with the analyses by aCGH and NGS regarding chromosome 7 gain. Taken together, the frequency of chromosomal alterations in PRNRP is similar to that in non-neoplastic tubular cells, and lower than that in PRCC. Our data suggest that PRNRP has a different tumorigenesis and is a distinct entity from PRCC.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Cromosomas Humanos Par 7 / Cromosomas Humanos Par 17 / Hibridación Fluorescente in Situ / Cromosomas Humanos Y / Neoplasias Renales Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Virchows Arch Asunto de la revista: BIOLOGIA MOLECULAR / PATOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Cromosomas Humanos Par 7 / Cromosomas Humanos Par 17 / Hibridación Fluorescente in Situ / Cromosomas Humanos Y / Neoplasias Renales Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Virchows Arch Asunto de la revista: BIOLOGIA MOLECULAR / PATOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón