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Multi-microdialysis analytical system to monitor acetaminophen and its pharmacokinetic interaction with A. bidentata in rat blood, forelimb extensor muscle, brain striatum, and the knee joint cavity.
Sun, Chung-Kai; Huang, Andy C; Hsueh, Thomas Y; Ting, Chin-Tsung; Lin, Lie-Chwen; Tsai, Tung-Hu.
Afiliación
  • Sun CK; Institute of Traditional Medicine, College of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
  • Huang AC; Division of Urology, Department of Surgery, Taipei City Hospital Renai Branch, Taipei 106, Taiwan.
  • Hsueh TY; Division of Urology, Department of Surgery, Taipei City Hospital Renai Branch, Taipei 106, Taiwan; Department of Urology, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
  • Ting CT; Division of Gastrointestinal Surgery, Department of Surgery, Ren-Ai Branch, Taipei City Hospital, Taipei 106, Taiwan; General Education Center, University of Taipei, Taipei 100, Taiwan.
  • Lin LC; National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 112, Taiwan.
  • Tsai TH; Institute of Traditional Medicine, College of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; Graduate Institute of Acupuncture Science, China Medical University, Taichung 404, Taiwan; Graduate Institute of Law and Interdisciplinary Studies, College of Law, National Chengchi
J Pharm Biomed Anal ; 248: 116285, 2024 Sep 15.
Article en En | MEDLINE | ID: mdl-38878452
ABSTRACT
Acetaminophen (APAP), or paracetamol, is one of the most widespread and commonly used non-prescription pain medication in the world, and is effective at managing wide range of pain, including headache, muscle ache, and minor arthritic pain. While the pharmacokinetics of APAP is generally understood, there is a lack of data for its transfer ratio especially into the knee. A novel multi-microdialysis model was developed to simultaneously sample from blood, forelimb extensor muscle, brain striatum, and the knee joint cavity in the same experimental subject to investigate the potential interaction between APAP and Achyranthes bidentata Blume (A. bidentata), another widely used traditional Chinese medicininal herb especially for pain in the lower extremity. Rats were pre-treated with A. bidentata extract (ABex), APAP was then administered (60 mg/kg, i.v.), dialysates then subsequently analyzed using HPLC-PDA. Our analysis demonstrated that APAP concentrations, achieved after its administration either alone or in combination with ABex (1 and 3 g/kg, q.d. gavage), could be modelled effectively with a one-compartment model. The distribution ratio (AUCorgan/AUCblood) of blood-to-muscle, blood-to-brain and blood-to-knee was 0.372 ± 0.053, 0.277 ± 0.095 and 0.191 ± 0.042, respectively after administration of APAP (60 mg/kg, i.v.). No significant difference was observed between the pharmacokinetics of APAP administered alone and in combination with ABex; and APAP concentration exceed the half maximal effective concentration (EC50) in all sampled organs for close to 3 hours with one single dose of drug administration, providing evidence for its broad-range analgesic effect.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Ratas Sprague-Dawley / Articulación de la Rodilla / Acetaminofén Límite: Animals Idioma: En Revista: J Pharm Biomed Anal Año: 2024 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Ratas Sprague-Dawley / Articulación de la Rodilla / Acetaminofén Límite: Animals Idioma: En Revista: J Pharm Biomed Anal Año: 2024 Tipo del documento: Article