Feasible and economic DIY procedures of a hand-held fluorescence detector set-FluorDetector to assist laboratory course development.
Biochem Mol Biol Educ
; 2024 Jun 25.
Article
en En
| MEDLINE
| ID: mdl-38923792
ABSTRACT
Fluorescence-related experimental techniques play an important role in biochemistry, molecular biology, and cell biology. However, fluorescence-related experiments are rarely included in the laboratory courses of most Chinese universities. This is mainly due to the conflict between large class size (50-60 students in one room) and funding/space limitations to purchase and accommodate enough fluorescence detection equipment. Here, we proposed feasible and economical Do It Yourself (DIY) procedures of a hand-held fluorescence detector set-FluorDetector to support the development of laboratory courses. Tested on several samples, clear fluorescence signals could be directly observed by FluorDetector and photographed with a smartphone. In addition, FluorDetector was able to turn a conventional stereomicroscope into a fluorescence stereomicroscope, detecting fluorescence signals with clean background. FluorDetector is easy to make with a 3D printer, with an extremely low cost ($200 each) when compared with a commercial fluorescence microscope or fluorescence stereomicroscope, and almost as sensitive as a microplate reader in measuring fluorescence. Therefore, FluorDetector is a possible strategy to solve the problem and help to integrate fluorescence-related experimental modules in laboratory courses.
Texto completo:
1
Base de datos:
MEDLINE
Idioma:
En
Revista:
Biochem Mol Biol Educ
Año:
2024
Tipo del documento:
Article
País de afiliación:
China