Early changes in asporin levels in osteoarthritis of the temporomandibular joint.
J Oral Biosci
; 66(3): 546-553, 2024 Sep.
Article
en En
| MEDLINE
| ID: mdl-38936470
ABSTRACT
OBJECTIVES:
The present study aimed to elucidate the pathogenesis of temporomandibular joint (TMJ) osteoarthritis (TMJ-OA) in a mouse model. We investigated morphological and histological changes in the head of mandible cartilage and early immunohistochemical (IHC) changes in transforming growth factor (TGF)-ß, phosphorylated Smad-2/3 (p-Smad2/3), a TGF-ß signaling molecule, and asporin.METHODS:
TMJ-OA was induced in a mouse model through unilateral partial discectomy. Micro-computed tomography (micro-CT) and safranin-O staining were performed to morphologically and histologically evaluate the degeneration of the head of mandible caused by TMJ-OA. IHC staining for TGF-ß, p-Smad2/3, and asporin was performed to evaluate the changes in protein expression.RESULTS:
In the experimental group, three-dimensional (3D) morphometry revealed an enlarged head of mandible and safranin-O staining showed degeneration of cartilage tissue in the early stages of TMJ-OA compared to the control group. IHC staining revealed that TGF-ß, p-Smad2/3, and asporin expression increased in the head of mandible cartilage before the degeneration of cartilage tissue, and subsequently decreased for a short period.CONCLUSION:
The findings suggested a negative feedback relationship between the expression of asporin and the TGF-ß/Smad transduction pathway, which may be involved in the degeneration of the head of mandible in the early stages of TMJ-OA. Asporin is a potential biomarker of the early stages of TMJ-OA, which ultimately leads to the irreversible degeneration of TMJ tissues.Palabras clave
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Base de datos:
MEDLINE
Asunto principal:
Osteoartritis
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Inmunohistoquímica
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Factor de Crecimiento Transformador beta
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Modelos Animales de Enfermedad
Límite:
Animals
Idioma:
En
Revista:
J Oral Biosci
Año:
2024
Tipo del documento:
Article
País de afiliación:
Japón