Applications of phase-separating multi-bilayers in protein-membrane domain interactions.
Methods Enzymol
; 700: 275-294, 2024.
Article
en En
| MEDLINE
| ID: mdl-38971603
ABSTRACT
Synthetic model membranes are important tools to elucidate lipid domain and protein interactions due to predefined lipid compositions and characterizable biophysical properties. Here, we introduce a model membrane with multiple lipid bilayers (multi-bilayers) stacked on a mica substrate that is prepared through a spin-coating technique. The spin-coated multi-bilayers are useful in the study of phase separated membranes with a high cholesterol content, mobile lipids, microscopic and reversible phase separation, and easy conjugation with proteins, which make them a good model to study interactions between proteins and membrane domains.
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Base de datos:
MEDLINE
Asunto principal:
Membrana Dobles de Lípidos
Idioma:
En
Revista:
Methods Enzymol
Año:
2024
Tipo del documento:
Article