Depletion of lipid storage droplet-1 delays endoreplication progression and induces cell death in Drosophila salivary gland.
Arch Insect Biochem Physiol
; 116(3): e22132, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38993002
ABSTRACT
Perilipins are evolutionarily conserved from insects to mammals. Drosophila lipid storage droplet-1 (LSD-1) is a lipid storage droplet membrane surface-binding protein family member and a counterpart to mammalian perilipin 1 and is known to play a role in lipolysis. However, the function of LSD-1 during specific tissue development remains under investigation. This study demonstrated the role of LSD-1 in salivary gland development. Knockdown of Lsd-1 in the salivary gland was established using the GAL4/UAS system. The third-instar larvae of knockdown flies had small salivary glands containing cells with smaller nuclei. The null mutant Drosophila also showed the same phenotype. The depletion of LSD-1 expression induced a delay of endoreplication due to decreasing CycE expression and increasing DNA damage. Lsd-1 genetically interacted with Myc in the third-instar larvae. These results demonstrate that LSD-1 is involved in cell cycle and cell death programs in the salivary gland, providing novel insight into the effects of LSD-1 in regulating salivary gland development and the interaction between LSD-1 and Myc.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Glándulas Salivales
/
Muerte Celular
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Proteínas de Drosophila
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Larva
Límite:
Animals
Idioma:
En
Revista:
Arch Insect Biochem Physiol
/
Arch. insect biochem. physiol
/
Archives of insect biochemistry and physiology
Asunto de la revista:
BIOLOGIA
/
BIOQUIMICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Japón