Role of legumain in metabolic dysfunction, diagnosis, and prognosis of patients with atherosclerosis.
Medicine (Baltimore)
; 103(29): e38896, 2024 Jul 19.
Article
en En
| MEDLINE
| ID: mdl-39029045
ABSTRACT
BACKGROUND:
The purpose of this study was to investigate the role of legumain in metabolic dysfunction, diagnosis, and prognosis in patients with atherosclerosis.METHODS:
Plasma levels of legumain from patients with atherosclerosis (nâ =â 320) and healthy controls (nâ =â 320), expression of legumain in atheromatous plaque and secreted from monocyte-derived macrophages were measured using enzyme-linked-immunosorbent assay, reverse transcription-polymerase chain reaction, Western blot, immunohistochemistry, and fluorescence.RESULTS:
Data demonstrated that atherosclerotic patients had higher plasma level of legumain than healthy controls, which was a diagnostic and prognostic marker and corrected with the degree of atherosclerosis. It found that atheromatous plaque and endothelial cell had higher legumain expression than non-atherosclerotic arteries (controls). Legumain showed significantly increased secretion from pro-inflammatory M1 compared to pro-resolving M2 macrophages during monocyte-derived macrophages, which was localized to structures resembling foam cells.CONCLUSION:
In conclusion, our data indicate that legumain expression is upregulated in both plasma and plaques of patients with atherosclerosis, which is associated with metabolic dysfunction of endothelial cell and might be a diagnostic and prognostic marker of atherosclerosis.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Cisteína Endopeptidasas
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Biomarcadores
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Aterosclerosis
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Macrófagos
Límite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Medicine (Baltimore)
Año:
2024
Tipo del documento:
Article
País de afiliación:
China