A dual-signal mode electrochemical aptasensor based on tetrahedral DNA nanostructures for sensitive detection of citrinin in food using PtPdCo mesoporous nanozymes.
Food Chem
; 460(Pt 3): 140739, 2024 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-39116770
ABSTRACT
Citrinin (CIT) is a mycotoxin with nephrotoxicity and hepatotoxicity, presenting a significant threat to human health that is often overlooked. Therefore, a dual-signal mode (DPV and SWV) aptasensor for citrinin (CIT) detection was constructed based on tetrahedral DNA nanostructures (TDN) in this study. Furthermore, PtPdCo mesoporous nanozymes exhibit catalase-like catalytic functions, generating significant electrochemical signals through a Fenton-like reaction. Meanwhile their excellent Methylene Blue (MB) loading capability ensures independent dual signal outputs. The RecJf exonuclease-assisted (RecJf Exo-assisted) process can expand the linear detection range, enabling further amplification of the signal. Under optimized conditions, the constructed aptaensor exhibited excellent detection performance with limits of detection (LODs) of 7.67 × 10-3 ng·mL-1 (DPV mode) and 1.57 × 10-3 ng·mL-1 (SWV mode). Due to its multiple signal amplification and highly accurate dual-signal mode detection capability, this aptasensor shows promising potential for the in situ detection.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
ADN
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Técnicas Biosensibles
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Contaminación de Alimentos
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Citrinina
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Nanoestructuras
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Aptámeros de Nucleótidos
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Técnicas Electroquímicas
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Límite de Detección
Idioma:
En
Revista:
Food Chem
Año:
2024
Tipo del documento:
Article