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Multi-omics analyses reveal aberrant differentiation trajectory with WNT1 loss-of-function in type XV osteogenesis imperfecta.
Tan, Zhijia; Chen, Peikai; Zhang, Jianan; Shek, Hiu Tung; Li, Zeluan; Zhou, Xinlin; Zhou, Yapeng; Yin, Shijie; Dong, Lina; Feng, Lin; Wong, Janus Siu Him; Gao, Bo; To, Michael Kai Tsun.
Afiliación
  • Tan Z; Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518053, China.
  • Chen P; Clinical Research Center for Rare Diseases, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518083, China.
  • Zhang J; Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
  • Shek HT; Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518053, China.
  • Li Z; Clinical Research Center for Rare Diseases, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518083, China.
  • Zhou X; School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
  • Zhou Y; The AI and Big Data Lab, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518053, China.
  • Yin S; Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
  • Dong L; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
  • Feng L; Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518053, China.
  • Wong JSH; Clinical Research Center for Rare Diseases, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518083, China.
  • Gao B; Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518053, China.
  • To MKT; Clinical Research Center for Rare Diseases, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518083, China.
J Bone Miner Res ; 39(9): 1253-1267, 2024 Sep 02.
Article en En | MEDLINE | ID: mdl-39126373
ABSTRACT
Osteogenesis imperfecta (OI) is a group of severe genetic bone disorders characterized by congenital low bone mass, deformity, and frequent fractures. Type XV OI is a moderate to severe form of skeletal dysplasia caused by WNT1 variants. In this cohort study from southern China, we summarized the clinical phenotypes of patients with WNT1 variants and found that the proportion of type XV patients was around 10.3% (25 out of 243) with a diverse spectrum of phenotypes. Functional assays indicated that variants of WNT1 significantly impaired its secretion and effective activity, leading to moderate to severe clinical manifestations, porous bone structure, and enhanced osteoclastic activities. Analysis of proteomic data from human skeleton indicated that the expression of SOST (sclerostin) was dramatically reduced in type XV patients compared to patients with COL1A1 quantitative variants. Single-cell transcriptome data generated from human tibia samples of patients diagnosed with type XV OI and leg-length discrepancy, respectively, revealed aberrant differentiation trajectories of skeletal progenitors and impaired maturation of osteocytes with loss of WNT1, resulting in excessive CXCL12+ progenitors, fewer mature osteocytes, and the existence of abnormal cell populations with adipogenic characteristics. The integration of multi-omics data from human skeleton delineates how WNT1 regulates the differentiation and maturation of skeletal progenitors, which will provide a new direction for the treatment strategy of type XV OI and relative low bone mass diseases such as early onset osteoporosis.
Osteogenesis imperfecta is a rare disease characterized by low bone mass, frequent fractures, and long bone deformity. Type XV osteogenesis imperfect is an autosomal recessive disorder caused by WNT1 variants, while heterozygous variants of WNT1 result in early onset osteoporosis. In this cohort study, we summarized the clinical features of 25 patients diagnosed with type XV osteogenesis imperfect. The WNT1 variants were confirmed by genetic test. Molecular assays were conducted to reveal the impact of variants on WNT1 protein activity and bone structure. We then compared the protein levels in bone tissues isolated from the type XV patients and patients with mild deformity using proteomic method, and found that the expression of SOST, mainly produced by mature osteoblasts and osteocytes, was dramatically reduced in type XV patients. We further compared the global mRNA expression levels in the skeletal cells using single-cell RNA sequencing. Analyses of these data indicated that more immature progenitors were identified and maturation of osteocytes was impaired with WNT1 loss-of-function. Our study helps to understand the underlying pathogenesis of type XV osteogenesis imperfecta.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Osteogénesis Imperfecta / Diferenciación Celular / Proteína Wnt1 Límite: Adolescent / Animals / Child / Child, preschool / Female / Humans / Male Idioma: En Revista: J Bone Miner Res Asunto de la revista: METABOLISMO / ORTOPEDIA Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Osteogénesis Imperfecta / Diferenciación Celular / Proteína Wnt1 Límite: Adolescent / Animals / Child / Child, preschool / Female / Humans / Male Idioma: En Revista: J Bone Miner Res Asunto de la revista: METABOLISMO / ORTOPEDIA Año: 2024 Tipo del documento: Article País de afiliación: China