Single nucleotide polymorphisms in SEPALLATA 2 underlie fruit length variation in cucurbits.
Plant Cell
; 36(10): 4607-4621, 2024 Oct 03.
Article
en En
| MEDLINE
| ID: mdl-39133577
ABSTRACT
Complete disruption of critical genes is generally accompanied by severe growth and developmental defects, which dramatically hinder its utilization in crop breeding. Identifying subtle changes, such as single-nucleotide polymorphisms (SNPs), in critical genes that specifically modulate a favorable trait is a prerequisite to fulfill breeding potential. Here, we found 2 SNPs in the E-class floral organ identity gene cucumber (Cucumis sativus) SEPALLATA2 (CsSEP2) that specifically regulate fruit length. Haplotype (HAP) 1 (8G2667A) and HAP2 (8G2667T) exist in natural populations, whereas HAP3 (8A2667T) is induced by ethyl methanesulfonate mutagenesis. Phenotypic characterization of 4 near-isogenic lines and a mutant line showed that HAP2 fruits are significantly longer than those of HAP1, and those of HAP3 are 37.8% longer than HAP2 fruit. The increasing fruit length in HAP1-3 was caused by a decreasing inhibitory effect on CRABS CLAW (CsCRC) transcription (a reported positive regulator of fruit length), resulting in enhanced cell expansion. Moreover, a 7638G/A-SNP in melon (Cucumis melo) CmSEP2 modulates fruit length in a natural melon population via the conserved SEP2-CRC module. Our findings provide a strategy for utilizing essential regulators with pleiotropic effects during crop breeding.
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1
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
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Regulación de la Expresión Génica de las Plantas
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Cucumis sativus
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Polimorfismo de Nucleótido Simple
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Frutas
Idioma:
En
Revista:
Plant Cell
Asunto de la revista:
BOTANICA
Año:
2024
Tipo del documento:
Article