Protocol for quantifying drug sensitivity in 3D patient-derived ovarian cancer models.
STAR Protoc
; 5(3): 103274, 2024 Sep 20.
Article
en En
| MEDLINE
| ID: mdl-39172645
ABSTRACT
Three-dimensional (3D) ex vivo cultures allow the study of cancer progression and drug resistance mechanisms. Here, we present a protocol for measuring on-target drug sensitivity in a scaffold-free 3D culture system through quantification of apoptotic tumor cells. We provide detailed steps for sample processing, immunofluorescence staining, semi-high-throughput confocal imaging, and imaged-based quantification of 3D cultures. This protocol is versatile and can be applied in principle to any patient-derived material.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Ováricas
Límite:
Female
/
Humans
Idioma:
En
Revista:
STAR Protoc
/
STAR protocols
Año:
2024
Tipo del documento:
Article