Aspergillus fumigatus conidial surface-associated proteome reveals factors for fungal evasion and host immunity modulation.
Nat Microbiol
; 9(10): 2710-2726, 2024 Oct.
Article
en En
| MEDLINE
| ID: mdl-39191887
ABSTRACT
Aspergillus fumigatus causes aspergillosis and relies on asexual spores (conidia) for initiating host infection. There is scarce information about A. fumigatus proteins involved in fungal evasion and host immunity modulation. Here we analysed the conidial surface proteome of A. fumigatus, two closely related non-pathogenic species, Aspergillus fischeri and Aspergillus oerlinghausenensis, as well as pathogenic Aspergillus lentulus, to identify such proteins. After identifying 62 proteins exclusively detected on the A. fumigatus conidial surface, we assessed null mutants for 42 genes encoding these proteins. Deletion of 33 of these genes altered susceptibility to macrophage, epithelial cells and cytokine production. Notably, a gene that encodes a putative glycosylasparaginase, modulating levels of the host proinflammatory cytokine IL-1ß, is important for infection in an immunocompetent murine model of fungal disease. These results suggest that A. fumigatus conidial surface proteins are important for evasion and modulation of the immune response at the onset of fungal infection.
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1
Base de datos:
MEDLINE
Asunto principal:
Aspergilosis
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Aspergillus fumigatus
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Esporas Fúngicas
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Proteínas Fúngicas
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Proteoma
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Evasión Inmune
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Nat Microbiol
Año:
2024
Tipo del documento:
Article
País de afiliación:
Brasil