[Diagnosis and evaluation of the response to treatment of chronic myeloid leukaemia in Mali by fluorescent in situ hybridization]. / Diagnostic et Évaluation de la Réponse Thérapeutique de la Leucémie Myéloïde Chronique au Mali par l'Hybridation in situ Fluorescente.
Health Sci Dis
; 23(7): 6-9, 2022 Jul.
Article
en Fr
| MEDLINE
| ID: mdl-39239208
ABSTRACT
Objective:
Chronic myeloid leukemia (CML) is a hematologic malignancy characterized by the presence of the Philadelphia chromosome or its molecular equivalent, the BCR/ABL1 fusion gene. Diagnosis and monitoring of CML are done by detecting this chromosome, the BCR/ABL1 gene, or the BCR/ABL1 transcript. In Mali, genetic tools of diagnosis and follow-up are still lacking, so we did this study with the objectives of developing the FISH technique to diagnose, to follow up, and to characterize the cytogenetic profile of CML patients.Methods:
We carried out FISH technique by using the dual color dual fusion probe for BCR/ABL1 on interphase nuclei and metaphases. Slides were scanned with an epifluorescence microscope.Results:
A total of 25 patients (16 for diagnostic and 9 for follow-up) were included. We achieved a 92% success rate for obtaining metaphases. The BCR/ABL1 gene fusion signal was present in 22 patients. Among those 22 patients, 16 presented a typical signal pattern and 6 presented atypical signal patterns.Conclusion:
We set up the FISH technique in Mali for the diagnosis and the follow-up of CML patients and identified atypical translocation of t(9;22).
Texto completo:
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Base de datos:
MEDLINE
Idioma:
Fr
Revista:
Health Sci Dis
Año:
2022
Tipo del documento:
Article
País de afiliación:
Mali