Effect of ß-1,4-GalTI on the biological function of astrocytes treated by LPS.

ABSTRACT

Inflammation of the central nervous system (CNS) is a common feature of neurological disorders and infections, playing a crucial role in the development of CNS-related conditions. CNS inflammation is primarily regulated by glial cells, with astrocytes being the most abundant type in the mammalian CNS. Numerous studies have demonstrated that astrocytes, as immunocompetent cells, perform diverse and complex functions in both health and disease. Glycosylation, a critical post-translational modification of proteins, regulates numerous biological functions. The expression and activity of glycosyltransferases, the enzymes responsible for glycosylation, are closely associated with the pathogenesis of various diseases. ß-1,4-GalTI, a mammalian glycosyltransferase, plays a significant role in cell-cell interactions, adhesion, and migration. Although many studies have focused on ß-1,4-GalTI, few have explored its effects on astrocyte function. In this study, we constructed lentiviral vectors for both interference and overexpression of ß-1,4-GalTI and discovered that ß-1,4-GalTI knockdown inhibited astrocyte migration and proliferation, while its overexpression promoted these processes. Concurrently, ß-1,4-GalTI knockdown reduced the expression of TNF-α, IL-1ß, and IL-6, whereas overexpression enhanced the expression of these cytokines. These findings suggest that modulating ß-1,4-GalTI activity can influence the molecular functions of astrocytes and provide a theoretical foundation for further research into ß-1,4-GalTI as a potential therapeutic target in astrocyte-mediated inflammation.