Horseradish peroxidase application to the protein transport investigations and alkaline phosphatase ultralocalization at the kidney barrier level.

ABSTRACT

Horseradish peroxidase was used to the precise investigation of low molecular protein transport across the kidney barrier. Simultaneously on the parallel material alkaline phosphatase activity was revealed, as this enzyme is connected with the transport processes through cell membranes. The applied reaction diaminebenzidine has shown infiltration spots for low molecular protein at the glomercular barrier and visualized tubular reabsorption of peroxidase in proximal convoluted tubule. Alkaline phosphatase activity has been observed on the surface of podocytes foot processes. On the surface of proximal convoluted tubules brush border and in canals formed with cells basal membranes investigations high activity of alkaline phosphatase was also observed.