Intestinal Biosynthesis of apolipoproteins in the rat: apoE and apoA-I mRNA translation and regulation.

ABSTRACT

Rat intestinal poly(A) RNA was translated in wheat germ and reticulocyte lysate systems in vitro. ApoA-I and apoE were demonstrated to be specific products by immunoprecipitation and fractionation on sodium dodecyl sulfate acrylamide gels. They were identical in size to the respective products from rat liver. In pulse-labeling studies, apoE was shown to be synthesized by slices of rat intestine in situ. Furthermore, a high cholesterol diet stimulated the synthesis of apoE and apoA-I at the pretranslational level.