Analysis of carminomycin in human serum by fluorometric high-performance liquid chromatography.
J Chromatogr
; 223(1): 155-64, 1981 Apr 10.
Article
en En
| MEDLINE
| ID: mdl-7251757
ABSTRACT
A method is given for the determination of carminomycin (CMM) and a major metabolite carminomycinol (CMMOH) in serum from cancer patients after intravenous administration of carminomycin as the free drug. CMM and CMMOH are extracted from serum with chloroform, the extract evaporated and the residue dissolved in methanol. High performance liquid chromatography analysis utilized a C18 microBondapak reversed-phase column eluted with 0.1 mol/l acetate buffer (pH 4)-acetonitrile (6040, v/v) with fluorescence detection. The assay is linear, reproducible, and precise with a limit of detection of 2 ng/ml. Representative serum levels of CMM and CMMOH in a cancer patients are presented.
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Base de datos:
MEDLINE
Asunto principal:
Carubicina
/
Daunorrubicina
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr
Año:
1981
Tipo del documento:
Article