Heterogeneity of liver-kidney microsomal autoantibodies in chronic hepatitis C and D virus infection.
Gastroenterology
; 108(2): 455-62, 1995 Feb.
Article
en En
| MEDLINE
| ID: mdl-7835588
ABSTRACT
BACKGROUND/AIMS:
Anti-liver-kidney microsomal (LKM) autoantibodies occur in a proportion of patients with chronic hepatitis C and D infections. Because of different immunofluorescence patterns, antibodies in hepatitis C and D were termed LKM-1 and LKM-3, respectively. The aim of the present study was to evaluate the different specificities of LKM-1 and LKM-3 antibodies.METHODS:
Forty-nine samples of LKM-1 sera and 16 samples of LKM-3 sera were studied for reactivity against rat and human liver microsomal proteins by immunofluorescence, enzyme-linked immunosorbent assay, and Western blot.RESULTS:
Thirty-four percent of the LKM-1 sera reacted with 50-kilodalton cytochrome P4502D6 in Western blot. In addition, a proportion of the sera recognized either a 59- or 70-kilodalton antigen, and 45% of the sera did not react in Western blot. Recently, the major LKM-3 antigen was identified as an autoepitope expressed on uridine diphosphate-glucuronosyltransferases (UGT). Seven LKM-3-positive sera reacted with recombinant rabbit family one UGT. None of the anti-LKM-1-positive hepatitis C sera reacted with UGT. Antibody reactivity against liver microsomal proteins in enzyme-linked immunosorbent assay ended when antigens were pretreated with sodium dodecyl sulfate, confirming that antibodies recognize conformational epitopes.CONCLUSIONS:
LKM-1 antibodies in hepatitis C are more heterogeneous and react with different antigens compared with LKM-3 antibodies in hepatitis D.
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Base de datos:
MEDLINE
Asunto principal:
Autoanticuerpos
/
Hepatitis D
/
Hepatitis C
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Gastroenterology
Año:
1995
Tipo del documento:
Article
País de afiliación:
Alemania