Possible involvement of nitric oxide in acetylcholine-induced increase of intracellular Ca2+ concentration and catecholamine release in bovine adrenal chromaffin cells.
Jpn J Pharmacol
; 65(1): 73-7, 1994 May.
Article
en En
| MEDLINE
| ID: mdl-8089933
ABSTRACT
The role of nitric oxide (NO) in neurotransmitter release was studied using bovine adrenal medullary chromaffin cells. L-Arginine and sodium nitroprusside (SNP) slightly increased the intracellular free calcium concentration ([Ca2+]i), and the effects of the agents were dependent on the presence of the extracellular Ca2+ ([Ca2+]o), but were not blocked by verapamil (30 microM) or diltiazem (30 microM). SNP enhanced the acetylcholine (ACh)-induced rise in [Ca2+]i in the presence but not in the absence of [Ca2+]o. The effects of L-arginine but not those of SNP were inhibited by N omega-nitro-L-arginine (L-NNA). L-NNA significantly reduced the ACh-induced rise in [Ca2+]i and catecholamine (CA) release, and the reduction was restored by L-arginine but not by D-arginine. These results suggest a possible involvement of NO in ACh-induced [Ca2+]i rise and CA release in bovine adrenal chromaffin cells.
Buscar en Google
Base de datos:
MEDLINE
Asunto principal:
Acetilcolina
/
Catecolaminas
/
Calcio
/
Sistema Cromafín
/
Óxido Nítrico
Límite:
Animals
Idioma:
En
Revista:
Jpn J Pharmacol
Año:
1994
Tipo del documento:
Article
País de afiliación:
Japón