SREBP transcriptional activity is mediated through an interaction with the CREB-binding protein.
Genes Dev
; 10(22): 2903-11, 1996 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-8918891
ABSTRACT
The sterol regulatory element binding proteins (SREBP-1 and -2) activate transcription of genes whose products are involved in the cellular uptake and synthesis of cholesterol. Although considerable effort has been exerted to define the events regulating the levels of active SREBP, little is known about the transcriptional cofactors mediating SREBP function. In an unbiased search for potential coactivators of SREBP, we isolated a protein of 265 kD from HeLa cells that directly bound SREBP-1 and SREBP-2. Peptide sequencing and Western blot analysis established that the 265-kD protein was CBP (CREB-binding protein), a recently identified transcriptional coactivator. The putative activation domain of SREBP was shown to bind specifically to amino-terminal domains of recombinant CBP and p300 (a CBP-related protein). Moreover, transfection studies demonstrated that CBP enhances the ability of SREBP to activate transcription of reporter genes in HeLa cells. Together, these data suggest that CBP mediates SREBP transcriptional activity, thus revealing a new step in the biochemical pathway regulating cholesterol metabolism.
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Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Transcripción Genética
/
Proteínas Nucleares
/
Transactivadores
/
Proteínas Potenciadoras de Unión a CCAAT
/
Proteínas de Unión al ADN
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Genes Dev
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos