Inhibition of protein phosphatases induces IGF-1-blocked neurotrophin-insensitive neuronal apoptosis.
FEBS Lett
; 398(1): 106-12, 1996 Nov 25.
Article
en En
| MEDLINE
| ID: mdl-8946962
ABSTRACT
We have previously described the marine toxin okadaic acid (OKA) to be a potent neurotoxin for cultured rat cerebellar neurons. Here we show that OKA-induced neurodegeneration involves the DNA fragmentation characteristic of apoptosis and is protein synthesis-dependent. DNA fragmentation and neurotoxicity correlated with inhibition of protein phosphatase (PP) 2A rather than PP1 activity. Neurotrophins NT-3 and BDNF failed to protect from OKA-induced apoptotic neurotoxicity that was, however, totally prevented by insulin-like growth factor-1. Neuronal death by OKA was significantly reduced by protein kinase C inhibitors and by the L-type calcium channel agonist Bay K8644, while it was potentiated by the reduction of free extracellular calcium concentrations.
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Base de datos:
MEDLINE
Asunto principal:
Factor I del Crecimiento Similar a la Insulina
/
Fosfoproteínas Fosfatasas
/
Factor Neurotrófico Derivado del Encéfalo
/
Factores de Crecimiento Nervioso
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1996
Tipo del documento:
Article
País de afiliación:
España