Molecular cloning of the complementary DNA for an additional member of the family of aortic aneurysm antigenic proteins.
J Vasc Surg
; 26(2): 313-8, 1997 Aug.
Article
en En
| MEDLINE
| ID: mdl-9279320
ABSTRACT
PURPOSE:
We have purified and partially sequenced a protein from the adventitia of the human aorta (aortic aneurysm antigenic protein 40 kDa; AAAP-40) that has homologies to bovine aortic microfibril-associated glycoprotein (MAGP-36). It is immunoreactive with immunoglobulin G (IgGs) purified from the serum and aortic wall of patients with abdominal aortic aneurysms. AAAP-40 and MAGP-36 have fibrinogen-like and vitronectin-like motifs. Screening an expression library constructed from human aortic adventitial messenger RNA has resulted in the cloning of three complementary DNAs whose gene products are immunoreactive with immunoglobulin G from patients with abdominal aortic aneurysms. Two strongly resemble each other and have been described separately. The purpose of this article is to report the third clone.METHODS:
Messenger RNA from a specimen of human aortic aneurysmal adventitia was reverse-transcribed for insertion into the phagemid Uni Zap XR (Stratagene). A strain of Escherichia coli, engineered for expression (XL 1-Blue MFR', Stratagene), was transfected, and rabbit antihuman vitronectin antibody as used to identify positive clones. Sequencing of the positive clones was performed by the Core Laboratories at Columbia University.RESULTS:
The hypothetical protein of rAAAP-CL4 (clone 4) shares sequence motifs with known microfibril-associated glycoproteins (MAGPs). The recombinant protein (rAAAP-CL4) is immunoreactive with serum from patients (three of four abdominal aortic aneurysm sera). In addition, similarities have been detected with immunoglobulins of the kappa family and with a protein from cytomegalovirus that is a potential molecular mimic.CONCLUSIONS:
There may several members of a novel family of human aortic autoantigenic proteins implicated in abdominal aortic aneurysm disease.
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Base de datos:
MEDLINE
Asunto principal:
Homología de Secuencia de Ácido Nucleico
/
Proteínas de la Matriz Extracelular
/
Aneurisma de la Aorta Abdominal
/
ADN Complementario
/
Proteínas Contráctiles
/
Tejido Elástico
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Vasc Surg
Asunto de la revista:
ANGIOLOGIA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos