Nucleotide sequence of the chicken HMGI-C cDNA and expression of the HMGI-C and IGF1 genes in autosomal dwarf chicken embryos.
Biochim Biophys Acta
; 1399(1): 83-7, 1998 Jul 30.
Article
en En
| MEDLINE
| ID: mdl-9714752
ABSTRACT
Mutations in the genes for high mobility group protein I-C (HMGI-C) and insulin-like growth factor 1 (IGF1) are known to be responsible for dwarf phenotypes in the mouse. Because the locus for autosomal dwarfism (adw) in the chicken maps to a region which is syntenic to a region in the human and mouse in which the HMGI-C and IGF1 genes are located, HMGI-C and IGF1 are likely candidate genes for adw in the chicken. In this study their possible role in the establishment of this phenotype has been investigated. We have cloned and sequenced the complete coding region of the chicken HMGI-C cDNA. Comparison with its human counterpart revealed a nucleotide sequence conservation of 84%. Only nine amino acids are present principally in the N-terminal segment before the first DNA-binding domain. Northern blot analysis showed no difference in the expression of the HMGI-C gene between adw and wild-type chicken embryos. Also no mutations in either the HMGI-C or the IGF1 RNA nucleotide sequence were detected in adw chicken embryos.
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Base de datos:
MEDLINE
Asunto principal:
Proteínas del Grupo de Alta Movilidad
/
Pollos
/
ADN Complementario
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1998
Tipo del documento:
Article
País de afiliación:
Países Bajos