Recombinant Helicobacter pylori urease activates primary mucosal macrophages.
J Infect Dis
; 178(5): 1516-20, 1998 Nov.
Article
en En
| MEDLINE
| ID: mdl-9780278
ABSTRACT
Helicobacter pylori urease is absorbed into the gastric mucosa at sites of inflammation, but whether the enzyme activates mucosal macrophages is not known. Because mucosal macrophages differ phenotypically and functionally from blood monocytes, whether recombinant H. pylori urease (rUrease) activated purified lamina propria macrophages in vitro was investigated. rUrease (1-10 microgram/mL) induced primary mucosal macrophages to produce interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha but not IL-8 proteins in a dose-dependent manner (P<.05 to P<.001). Quantitative reverse transcriptase-polymerase chain reaction using capillary electrophoresis laser-induced fluorescence showed that rUrease (0.1-10 microgram/mL) also induced dose-dependent expression of IL-1beta, IL-6, and TNF-alpha but not IL-8 mRNA (P<.05), suggesting that rUrease-induced production of certain cytokines is regulated at the level of gene transcription. These findings indicate that the ability of H. pylori urease to activate mucosal macrophages, resulting in production of proinflammatory cytokines, may be involved in the pathogenesis of H. pylori-associated mucosal inflammation.
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Base de datos:
MEDLINE
Asunto principal:
Ureasa
/
Helicobacter pylori
/
Activación de Macrófagos
/
Macrófagos
Límite:
Humans
Idioma:
En
Revista:
J Infect Dis
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos