Development of a rapid method for quantitative evaluation of Mycobacterium tuberculosis growth based on competitive polymerase chain reaction.
J Med Microbiol
; 47(10): 933-6, 1998 Oct.
Article
en En
| MEDLINE
| ID: mdl-9788819
ABSTRACT
A competitive polymerase chain reaction (cPCR) assay for the quantitative evaluation of Mycobacterium tuberculosis growth was developed based on co-amplification of genomic DNA and a modified DNA fragment derived from a well-conserved region of the 16S rRNA gene. There was a good correlation between the number of DNA copies in the sample, indicated by competitive PCR, and the number of colony forming units determined by conventional culture methods.
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Base de datos:
MEDLINE
Asunto principal:
ADN Bacteriano
/
Reacción en Cadena de la Polimerasa
/
Mycobacterium tuberculosis
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Med Microbiol
Año:
1998
Tipo del documento:
Article
País de afiliación:
Italia