Mitigation effect of alpha-tocopherol and thermo-priming in Brassica napus L. under induced mercuric chloride stress.

Soil pollution with heavy metals has grown to be a big hassle, leading to the loss in farming production particularly in developing countries like Pakistan, where no proper channel is present for irrigation and extraction of these toxic heavy metals. The present study aims to ameliorate the damages caused by heavy metal ions (Hg-Mercury) on rapeseed (Brassica napus L.) via a growth regulator (α-tocopherol 150 mg/L) and thermopriming technique at 4 °C and 50 °C to maintain plant agronomical and physiological characteristics. In pot experiments, we designed total of 11 treatments viz.( T0 (control), T1 (Hg4ppm), T2 (Hg8ppm), T3 (Hg4ppm + 4 °C), T4 (Hg4ppm + 4 °C + tocopherol (150 m/L)), T5 (Hg4ppm + 50 °C), T6 (Hg4ppm + 50 °C + tocopherol (150 mg/L)), T7 (Hg8ppm + 4 °C), T8 (Hg8ppm + 4 °C + tocopherol (150 mg/L)), T9 (Hg8ppm + 50 °C), T10 (Hg8ppm + 50 °C + tocopherol (150 mg/L) the results revealed that chlorophyll content at p < 0.05 with growth regulator and antioxidant enzymes such as catalase, peroxidase, and malondialdehyde enhanced up to the maximum level at T5 = Hg4ppm + 50 °C (50 °C thermopriming under 4 ppm mercuric chloride stress), suggesting that high temperature initiate the antioxidant system to reduce photosystem damage. However, protein, proline, superoxide dismutase at p < 0.05, and carotenoid, soluble sugar, and ascorbate peroxidase were increased non-significantly (p > 0.05) 50 °C thermopriming under 8 ppm high mercuric chloride stress (T9 = Hg8ppm + 50 °C) representing the tolerance of selected specie by synthesizing osmolytes to resist oxidation mechanism. Furthermore, reduction in % MC (moisture content) is easily improved with foliar application of α-tocopherol and 50 °C thermopriming and 4 ppm heavy metal stress at T6 = Hg4ppm + 50 °C + α-tocopherol (150 mg/L), with a remarkable increase in plant vigor and germination energy. It has resulted that the inhibitory effect of only lower concentration (4 ppm) of heavy metal stress was ameliorated by exogenous application of α-tocopherol and thermopriming technique by synthesizing high levels of proline and antioxidant activities in maintaining seedling growth and development on heavy metal contaminated soil.

Inoculation of heavy metal resistant bacteria alleviated heavy metal-induced oxidative stress biomarkers in spinach (Spinacia oleracea L.).

Most vegetable crops are severely affected by the uptake of heavy metals from the soil. Heavy metals in vegetable bodies generate reactive oxygen species (ROS) that unbalance the antioxidant defense system. This study was initiated to determine the physiological and biochemical characteristics of spinach plants grown on soil contaminated with heavy metals and responding to Bacillus cereus and Bacillus aerius were isolated from soil contaminated with heavy metals. Heavy metal contamination led to a significant reduction in seed germination, seedling biomass, protein, and total nitrogen content of spinach plants grown in contaminated soils compared to control soils. In contrast, a significant increase in the content of metallothioneins and antioxidant enzymes was observed. Plants inoculated with B. cereus and B. aerius significantly reduced the oxidative stress induced by heavy metals by improving seed germination (%), seedling growth, nitrogen, and protein content. The content of metallothioneins and the activities of antioxidant enzymes were reduced in spinach plants grown from seeds inoculated with bacterial strains. In addition, plants inoculated with, B. cereus and B. aerius showed greater stomata opening than plants grown on soil contaminated with heavy metals, whose stomata were almost closed. These results suggested that both bacterial strains enhanced plant growth by reducing oxidative stress caused by metals.

Evaluation of heavy metal accumulation and tolerance in oxalic acid-treated Phragmites australis wetlands for textile effluent remediation.

Water contamination with metals poses significant environmental challenges. The occurrence of heavy metals (HMs) prompts modifications in plant structures, emphasizing the necessity of employing focused safeguarding measures. Cadmium (Cd), lead (Pb), and chromium (Cr) emerge as particularly menacing toxins due to their high accumulation potential. Increasing the availability of organic acids is crucial for optimizing toxic metal removal via phytoremediation. This constructed wetland system (CWs) was used to determine how oxalic acid (OA) treatments of textile wastewater (WW) effluents affected morpho-physiological characteristics, antioxidant enzyme activity, oxidative stress, and HM concentrations in Phragmites australis. Multiple treatments, comprising the application of OA at a concentration of 10 mM and WW at different dilutions (25%, 50%, 75%, and 100%), were employed, with three replications of each treatment. WW stress decreased chlorophyll and carotenoid content, and concurrently enhanced HMs adsorption and antioxidant enzyme activities. Furthermore, the application of WW was found to elevate oxidative stress levels, whereas the presence of OA concurrently mitigated this oxidative stress. Similarly, WW negatively affected soil-plant analysis development (SPAD) and the total soluble proteins (SP) in both roots and shoots. Conversely, these parameters showed improvement with OA treatments. P. australis showed the potential to enhance HM accumulation under 100% WW stress. Specifically, there is an increase in root SP ranging from 9% to 39%, an increase in shoot SP from 6% to 91%, and an elevation in SPAD values from 4% to 64% compared to their respective treatments lacking OA inclusion. The OA addition resulted in decreased EL contents in the root and shoot by 10%-19% and 13%-15%, MDA by 9%-14% and 9%-20%, and H2O2 by 14%-21% and 9%-17%, in comparison to the respective treatments without OA. Interestingly, the findings further revealed that the augmentation of OA also contributed to an increased accumulation of Cr, Cd, and Pb. Specifically, at 100% WW with OA (10 mM), the concentrations of Cr, Pb, and Cd in leaves rose by 164%, 447%, and 350%, in stems by 213%, 247%, and 219%, and in roots by 155%, 238%, and 195%, respectively. The chelating agent oxalic acid effectively alleviated plant toxicity induced by toxins. Overall, our findings demonstrate the remarkable tolerance of P. australis to elevated concentrations of WW stress, positioning it as an eco-friendly candidate for industrial effluent remediation. This plant exhibits efficacy in restoring contaminants present in textile effluents, and notably, oxalic acid emerges as a promising agent for the phytoextraction of HMs.

HMs stress decreased the physiology and morphology of Phragmites australis L.OA improved the photosynthetic pigments and antioxidant enzymesHMs accumulation and bioavailability increased under OAPhragmites australis L. showed higher efficacy for textile effluent treatment under OA.

Effect of green-synthesized copper oxide nanoparticles on growth, physiology, nutrient uptake, and cadmium accumulation in Triticum aestivum (L.).

Cadmium (Cd) stress in crops has been serious concern while little is known about the copper oxide nanoparticles (CuO NPs) effects on Cd accumulation by crops. This study investigated the effectiveness of CuO NPs in mitigating Cd contamination in wheat (Triticum aestivum L.) cultivation through a pot experiment, presenting an eco-friendly solution to a critical agricultural concern. The CuO NPs, synthesized using green methods, exhibited a circular shape with a crystalline structure and a particle size ranging from 8 to 12 nm. The foliar spray of CuO NPs was applied in four different concentrations i.e. control, 25, 50, 75, 100 mg/L. The obtained data demonstrated that, in comparison to the control group, CuO NPs had a beneficial influence on various growth metrics and straw and grain yields of T. aestivum. The green CuO NPs improved T. aestivum growth and physiology under Cd stress, enhanced selected enzyme activities, reduced oxidative stress, and decreased malondialdehyde levels in the T. aestivum plants. CuO NPs lowered Cd contents in T. aestivum tissues and boosted the uptake of essential nutrients from the soil. Overall, foliar applied CuO NPs were effective in minimizing Cd contents in grains thereby reducing the health risks associated with Cd excess in humans. However, more in depth studies with several plant species and application methods of CuO NPs are required for better utilization of NPs in agricultural purposes.

Phytoremediation of dinitrophenol from wastewater by atriplex lentiformis: effect of salicylic acid.

Quail bush [Atriplex lentiformis (Torr.) S. Wats] plants were used in removing 2, 4-dinitrophenol (DNP) from wastewater in a hydroponic experiment. The hydroponic system contained three doses of DNP, i.e., 0, 10, and 20 mg L-1. Quail bush plants were sprayed with 0.1 mM salicylic acid (SA) to study its role in resisting DNP toxicity. DNP significantly (p < 0.05) reduced plant growth. Exposure of A. lentiformis plants to 20 mg L-1 of DNP reduced the total chlorophyl and relative water content by 39 and 24%, respectively. SA improved the antioxidant defense in terms of ascorbate peroxidase (APX) and polyphenol oxidase (PPO) activities. SA alleviated DNP toxicity by enhancing the production of osmoprotectants, e.g.,proline, phenols, and carbohydrates. SA enhanced the removal efficiency of DNP and the highest removal efficiency (96%) was recorded in the plants sprayed with SA and grown on 10 mg L-1 of DNP. A. lentiformis is a halophytic plant that has good physiological characteristics to resist 2, 4-dinitrophenol toxicity in wastewaters and is qualified to purify water from these harmful compounds. Exogenous application of 0.1 mM SA increased the defense system in A. lentiformis against 2, 4-dinitrophenol toxicity and enhanced the removal efficiency.

2, 4-dinitrophenol inhibited the synthesis of photosynthetic pigments.Salicylic acid protects the vital bio-compounds in plant cells.Atriplex plants are able to remove (96%) of 2, 4-dinitrophenol from the wastewater.Atriplex plants have a strong antioxidant defense enable them to survive in wastewater.

Impact of sewage water irrigation on Datura innoxia grown in sandy loam soil.

BACKGROUND: A potential solution for recycling and reusing the massively produced sewage water (SW) is to irrigate certain plants instead of highly cost recycling treatment. Although the extensive and irrational application of SW may cause environmental pollution thus, continual monitoring of the redox status of the receiver plant and the feedback on its growth under application becomes an emergent instance. The impact of SW, along with well water (WW) irrigation of medicinal plant, Datura innoxia, was monitored by some physio-biochemical indices. RESULTS: The SW application amplified the growth, yield, minerals uptake, and quality of D. innoxia plants compared to the WW irrigated plants. The total chlorophyll, carotenoid, non-enzymatic antioxidants, viz. anthocyanin, flavonoids, phenolic compounds, and total alkaloids increased by 85, 38, 81, 50, 19, and 37%, respectively, above WW irrigated plants. The experiment terminated in enhanced leaf content of N, P, and K by 43, 118, and 48%, respectively. Moreover, stimulation of carbon and nitrogen metabolites in terms of proteins, soluble sugars, nitrate reductase (NR) activity, and nitric oxide (NO) content showed significant earliness in flowering time. The SW application improved not only Datura plants' quality but also soil quality. After four weeks of irrigation, the WW irrigated plants encountered nutrient deficiency-induced stress evidenced by the high level of proline, H2O2, and MDA as well as high enzyme capabilities. Application of SW for irrigation of D. innoxia plant showed the improvement of secondary metabolites regulating enzyme phenylalanine ammonia-lyase (PAL), restored proline content, and cell redox status reflecting high optimal condition for efficient cellular metabolism and performance along the experiment duration. CONCLUSIONS: These evidences approved the benefits of practicing SW to improve the yield and quality of D. innoxia and the feasibility of generalization on multipurpose plants grown in poor soil.

Hydrogen peroxide pretreatment assisted phytoremediation of sodium dodecyl sulfate by Juncus acutus L.

BACKGROUND: Sodium Dodecyl Sulfate (SDS) an anionic surfactant pollutant has emerged as a serious hazard to the aquatic and terrestrial environment. Due to physical and chemical methodological difficulties for SDS removal, phytoremediation techniques are efficient alternative strategies to tackle such adversities. Juncus acutus L. (J. acutus) is a pioneer wetland species that has been recently exploited for phytoremediation purposes. To our knowledge, the role of exogenous hydrogen peroxide (H2O2), in improving the phytoextraction of SDS has not been examined yet. In this study, pretreatment foliar spray of H2O2 (15 mM) combined with two levels of SDS (50 and 100 ppm) in water culture was evaluated to remove SDS contamination and add value to the phytoremediation process. RESULTS: The outcomes revealed that J. acutus has considerable translocation and bioaccumulation abilities for SDS and can be utilized as an appropriate hyperaccumulator in SDS-contaminated sites. However, the involvement of H2O2 extended phytoremediation capacity and successive removal of SDS. H2O2 significantly assisted in increasing SDS remediation via more accumulation in J. acutus tissues by 29.9 and 112.4% and decreasing SDS concentration in culture media by 33.3 and 27.3% at 50 and 100 ppm SDS, respectively. Bioaccumulation factor (BCF) increased by 13.8 and 13.2%, while translocation factor (TCF) positively maximized by 82.4 and 76.2% by H2O2 application at 50 and 100 ppm SDS, respectively. H2O2 pretreatment could drive the decline in biochemical attributes in SDS-affected plants by modulating stress tolerance indices, pigments, water relations, proline content, enzymatic activities, and further, reduced oxidative stress in terms of electrolyte leakage, cellular H2O2, malondialdehyde (MDA) accumulation. CONCLUSIONS: H2O2 could play a potential role in maximizing phytoremediation capacity of SDS by J. acutus in polluted sites.

Physiological and molecular analysis of pitaya (Hylocereus polyrhizus) reveal up-regulation of secondary metabolites, nitric oxide, antioxidant defense system, and expression of responsive genes under low-temperature stress by the pre-treatment of hydrogen peroxide.

Low-temperature events are one of the leading environmental cues that considerably reduce plant growth and shift species biodiversity. Hydrogen peroxide (H2O2) is a signaling molecule that has a distinguished role during unfavorable conditions and shows outstanding perspectives in low-temperature stress. Herein, we elucidated the protective role and regulatory mechanism of H2O2 in alleviating the deleterious effects of low-temperature stress in pitaya plants. Micropropagated pitaya plants were cultured in Murashige and Skoog media supplemented with different levels of H2O2 (0, 5, 10, and 20 mM) and then exposed to low-temperature stress (5 °C for 24 h). H2O2 at 10 mM, improved low-temperature stress tolerance by relieving oxidative injuries and ameliorating growth parameters in terms of fresh weight (66.7%), plant length (16.7%), and pigments content viz., chlorophyll a (157.4%), chlorophyll b (209.1%), and carotenoids (225.9%). H2O2 counteracted the low-temperature stress by increasing amino acids (224.7%), soluble proteins (190.5%), and sugars (126.6%). Simultaneously, secondary metabolites like ascorbic acid (ASA), anthocyanins, phenolics, flavonoids, total antioxidant (TOA), and proline were also up-regulated by H2O2 (104.9%, 128.8%, 166.3%, 141.4%, and 436.4%, respectively). These results corresponded to the stimulative role triggered by H2O2 in boosting the activities of catalase (22.4%), ascorbate peroxidase (20.7%), superoxide dismutase (88.4%), polyphenol oxidase (60.7%), soluble peroxidase (23.8%), and phenylalanine ammonia-lyase (57.1%) as well as the expression level of HpCAT, HpAPX, HpSOD, HpPPO, and HpPAL genes, which may help to moderate low-temperature stress. In conclusion, our findings stipulate new insights into the mechanisms by which H2O2 regulates low-temperature stress tolerance in pitaya plants.

Combined exposure of PVC-microplastic and mercury chloride (HgCl2) in sorghum (Pennisetum glaucum L.) when its seeds are primed titanium dioxide nanoparticles (TiO2-NPs).

The present work studied the impact of different levels of PVC-microplastics (PVC-MPs), namely 0 (no PVC-MPs), 2, and 4 mg L-1, along with mercury (Hg) levels of 0 (no Hg), 10, and 25 mg kg-1 in the soil, while concurrently applying titanium dioxide-nanoparticles (TiO2-NPs) at 0 (no TiO2-NPs), 50, and 100 µg mL-1 to sorghum (Pennisetum glaucum L.) plants. This study aimed to examine plant growth and biomass, photosynthetic pigments and gas exchange characteristics, oxidative stress indicators, and the response of various antioxidants (enzymatic and non-enzymatic) and their specific gene expression, proline metabolism, the AsA-GSH cycle, and cellular fractionation in the plants. The research outcomes indicated that elevated levels of PVC-MPs and Hg stress in the soil notably reduced plant growth and biomass, photosynthetic pigments, and gas exchange attributes. However, PVC-MPs and Hg stress also induced oxidative stress in the roots and shoots of the plants by increasing malondialdehyde (MDA), hydrogen peroxide (H2O2), and electrolyte leakage (EL) which also induced increased compounds of various enzymatic and non-enzymatic antioxidants and also the gene expression and sugar content. Furthermore, a significant increase in proline metabolism, the AsA-GSH cycle, and the pigmentation of cellular components was observed. Although, the application of TiO2-NPs showed a significant increase in plant growth and biomass, gas exchange characteristics, enzymatic and non-enzymatic compounds, and their gene expression and also decreased oxidative stress. In addition, the application of TiO2-NPs enhanced cellular fractionation and decreased the proline metabolism and AsA-GSH cycle in P. glaucum plants. These results open new insights for sustainable agriculture practices and hold immense promise in addressing the pressing challenges of heavy metal contamination in agricultural soils.

Population status, habitat preferences and predictive current and future distributions of three endangered Silene species under changing climate.

One of the most crucial steps in the practical conservation of endangered endemic mountain plants is to address their population size status and habitat requirements concurrently with understanding their response to future global warming. Three endangered Silene species-Silene leucophylla Boiss., S. schimperiana Boiss., and S. oreosinaica Chowdhuri-in Egypt were the focus of the current study. These species were examined for population status change, habitat quality variables (topography, soil features, and threats), and predictive current and future distributions. To find population size changes, recent field surveys and historical records were compared. Using Random Forest (RF) and Canonical Correspondence Analysis (CCA), habitat preferences were assessed. To forecast present-day distribution and climate change response, an ensemble model was used. The results reported a continuous decline in the population size of the three species. Both RF and CCA addressed that elevation, soil texture (silt, sand, and clay fractions), soil moisture, habitat-type, chlorides, electric conductivity, and slope were among the important variables associated with habitat quality. The central northern sector of the Saint Catherine area is the hotspot location for the predictive current distribution of three species with suitable areas of 291.40, 293.10, and 58.29 km2 for S. leucophylla, S. schimperiana, and S. oreosinaica, respectively. Precipitation-related variables and elevation were the key predictors for the current distribution of three Silene species. In response to climate change scenarios, the three Silene species exhibited a gradual contraction in the predictive suitable areas with upward shifts by 2050 and 2070. The protection of these species and reintroduction to the predicted current and future climatically suitable areas are urgent priorities. Ex-situ conservation and raised surveillance, as well as fenced enclosures may catapult as promising and effective approaches to conserving such threatened species.

Effect of hesperidin on growth, photosynthesis, antioxidant systems and uptake of cadmium, copper, chromium and zinc by Celosia argentea plants.

Rapid industrialization and extensive agricultural practices are the major causes of soil heavy metal contamination, which needs urgent attention to safeguard the soils from contamination. However, the phytotoxic effects of excessive metals in plants are the primary obstacle to efficient phytoextraction. The present study evaluated the effects of hesperidin (HSP) on metals (Cu, Cd, Cr, Zn) phytoextraction by hyperaccumulator (Celosia argentea L.) plants. For this purpose, HSP, a flavonoid compound with strong antioxidant potential to assist metal phytoextraction was used under metal stress in plants. Celosia argentea plants suffered significant (P ≤ 0.001) oxidative damage due to the colossal accumulation of metals (Cu, Cd, Cr, Zn). However, HSP supplementation notably (P ≤ 0.001) abated ROS generation (O2•‒, •OH, H2O2), lipoxygenase activity, methylglyoxal production, and relative membrane permeability that clearly indicated HSP-mediated decline in oxidative injury in plants. Exogenous HSP improved (P ≤ 0.001) the production of non-protein thiol, phytochelatins, osmolytes, and antioxidant compounds. Further, HSP enhanced (P ≤ 0.001) H2S and NO endogenous production, which might have improved the GSH: GSSG ratio. Consequently, HSP-treated C. argentea plants had higher biomass alongside elevated metal accumulation mirrored as profound modifications in translocation factor (TF), bioaccumulation coefficient (BAC), and bioconcentration factor (BCF). In this context, HSP significantly enhanced TF of Cr (P ≤ 0.001), Cd (P ≤ 0.001), and Zn (P ≤ 0.01), while BAC of Cr (P ≤ 0.001), Cd (P ≤ 0.001), and Zn (P ≤ 0.001). Further, BCF was significant (P ≤ 0.05) only in plants grown under Cr-spiked soil. Overall, HSP has the potential for phytoremediation of metals by C. argentea, which might be a suitable strategy for metal-polluted soils.

Amelioration of chromium toxicity in wheat plants through exogenous application of nano silicon.

Chromium (Cr) contamination in agricultural soils poses a risk to crop productivity and quality. Emerging nano-enabled strategies show great promise in remediating soils contaminated with heavy metals and enhancing crop production. The present study was aimed to investigate the efficacy of nano silicon (nSi) in promoting wheat growth and mitigating adverse effects of Cr-induced toxicity. Wheat seedlings exposed to Cr (K2Cr2O7) at a concentration of 100 mg kg-1 showed significant reductions in plant height (29.56%), fresh weight (35.60%), and dry weight (38.92%) along with enhanced Cr accumulation in roots and shoots as compared to the control plants. However, the application of nSi at a concentration of 150 mg kg-1 showcased substantial mitigation of Cr toxicity, leading to a decrease in Cr accumulation by 27.30% in roots and 35.46% in shoots of wheat seedlings. Moreover, nSi exhibited the capability to scavenge oxidative stressors, such as hydrogen peroxide (H2O2), and malondialdehyde (MDA) and electrolyte leakage, while significantly enhancing gas exchange parameters, total chlorophyll content, and antioxidant activities (enzymatic and nonenzymatic) in plants grown in Cr-contaminated soil. This study further found that the reduced Cr uptake by nSi application was due to downregulating the expression of HMs transporter genes (TaHMA2 and TaHMA3), alongwith upregulating the expression of antioxidant-responsive genes (TaSOD and TaSOD). The findings of this investigation highlight the remarkable potential of nSi in ameliorating Cr toxicity. This enhanced efficacy could be ascribed to the distinctive size and structure of nSi, which augment its ability to counteract Cr stress. Thus, the application of nSi could serve as a viable solution for production of crops in metal contaminated soils, offering an effective alternative to time-consuming and costly remediation techniques.

Identification and expression analysis of SBP-Box-like (SPL) gene family disclose their contribution to abiotic stress and flower budding in pigeon pea (Cajanus cajan).

The SPL gene family (for Squamosa Promoter-binding like Proteins) represents specific transcription factors that have significant roles in abiotic stress tolerance, development and the growth processes of different plants, including initiation of the leaf, branching and development of shoot and fruits. The SPL gene family has been studied in different plant species; however, its role is not yet fully explored in pigeon pea (Cajanus cajan ). In the present study, 11 members of the CcSPL gene family were identified in C. cajan . The identified SPLs were classified into nine groups based on a phylogenetic analysis involving SPL protein sequences from C. cajan , Arabidopsis thaliana , Cicer arietinum , Glycine max , Phaseolus vulgaris , Vigna unguiculata and Arachis hypogaea . Further, the identification of gene structure, motif analysis, domain analysis and presence of cis -regulatory elements in the SPL family members were studied. Based on RNA-sequencing data, gene expression analysis was performed, revealing that CcSPL2.1, 3 and 13A were significantly upregulated for salt-tolerance and CcSPL14 and 15 were upregulated in a salt-susceptible cultivar. Real-time qPCR validation indicated that CcSPL3, 4, 6 and 13A were upregulated under salt stress conditions. Therefore, molecular docking was performed against the proteins of two highly expressed genes (CcSPL3 and CcSPL14 ) with three ligands: abscisic acid, gibberellic acid and indole-3-acetic acid. Afterward, their binding affinity was obtained and three-dimensional structures were predicted. In the future, our study may open avenues for harnessing CcSPL genes in pigeon pea for enhanced abiotic stress resistance and developmental traits.

Microenvironment created by Plantago lagopus L. may affect cover and diversity of coexisting species in urban vegetation.

Some plant species may exhibit new microenvironments which lead to significant changes in the cover and diversity of the coexisting species. In this investigation, we evaluated the effects of Plantago lagopus L. on the cover and diversity of the associated plant species in the urban vegetation. A total of 70 plots were conducted in sites with- and without this species in urban gardens. Cover of the associated species and different diversity indices including species richness, Shannon-Wiener, evenness, and Simpson indices were measured. The allelopathic potential of P. lagopus was verified using its rhizosphere and non-rhizosphere soils on two target species existing within the same environment. Some soil criteria and seed sizes of the associated species were also determined. Most of the coexisting weeds were reduced in terms of their cover in plots with Plantago. The reduction of plant diversity depended on its cover. Besides, the aboveground biomass was reduced in sites comprising Plantago. The degree of inhibition was not related to the seed size of the species found. This species reduced the incident solar radiation and the local temperature over the soil surface. The locations exhibiting such species contained lower contents of available potassium and zinc. Rhizosphere soil of P. lagopus substantially inhibited germination and growth of Amaranthus viridis, but it didn't do so for Medicago lupulina. Reduction in cover, diversity, and biomass of the urban weeds associated with P. lagopus may be related to the reduction of received solar radiation, soil temperature, and nutrient availability. The allelopathic potential of P. lagopus may have a partial role in this reduction. These results suggest that P. lagopus may create a microenvironment of new conditions not favorable for most of the coexisting species.

Responses of Pea (Pisum sativum L.) to Single and Consortium Bio-Fertilizers in Clay and Newly Reclaimed Soils.

The huge development of climatic change highly affects our crop production and soil fertility. Also, the rise in the uncontrolled, excessive use of chemical fertilizers diminishes the soil prosperity and generates pollutants, threatening all environmental life forms, including us. Replacement of these chemical fertilizers with natural ones is becoming an inevitable environmental strategy. In our study, we evaluated the responses of Pisum sativum L. to the action of single species and consortiums of plant growth-promoting bacteria (Azotobacter chroococcum, Bacillus megaterium, and Bacillus cerkularice) in clay and new reclaimed soil types in terms of phenotype, yield components, and physiological and biochemical responses. Data analysis showed single or consortium microbial inoculation significantly increased the measured traits under clay and calcareous sandy soils compared to the control. Shoot physiological and biochemical activities, and seed biochemical activities were significantly enhanced with the inoculation of pea seeds with three types of bacteria in both soil types. The bud numbers, fresh weight, and seeds' dry weight increased in seeds treated with A. chroococcum and B. megaterium in the sandy soil. Taken together, these findings suggested that the inoculation of plants with PGP bacteria could be used to diminish the implementation of chemical fertilizer and improve the goodness of agricultural products. These findings expand the understanding of the responsive mechanism of microbial inoculation under different soil types, especially at physiological and biochemical levels.

Ameliorative Effects of Exogenous Potassium Nitrate on Antioxidant Defense System and Mineral Nutrient Uptake in Radish (Raphanus sativus L.) under Salinity Stress.

Soil salinization has become a major issue around the world in recent years, as it is one of the consequences of climate change as sea levels rise. It is crucial to lessen the severe consequences of soil salinization on plants. A pot experiment was conducted to regulate the physiological and biochemical mechanisms in order to evaluate the ameliorative effects of potassium nitrate (KNO3) on Raphanus sativus L. genotypes under salt stress. The results from the present study illustrated that the salinity stress induced a significant decrease in shoot length, root length, shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, number of leaves per plant, leaf area chlorophyll-a, chlorophyll-b, total chlorophyll, carotenoid, net photosynthesis, stomatal conductance, and transpiration rate by 43, 67, 41, 21, 34, 28, 74, 91, 50, 41, 24, 34, 14, 26, and 67%, respectively, in a 40 day radish while decreased by 34, 61, 49, 19, 31, 27, 70, 81, 41, 16, 31, 11, 21, and 62%, respectively, in Mino radish. Furthermore, MDA, H2O2 initiation, and EL (%) of two varieties (40 day radish and Mino radish) of R. sativus increased significantly (P < 0.05) by 86, 26, and 72%, respectively, in the roots and also increased by 76, 106, and 38% in the leaves in a 40 day radish, compared to the untreated plants. The results also elucidated that the contents of phenolic, flavonoids, ascorbic acid, and anthocyanin in the two varieties (40 day radish and Mino radish) of R. sativus increased with the exogenous application of KNO3 by 41, 43, 24, and 37%, respectively, in the 40 day radish grown under the controlled treatments. Results indicated that implementing KNO3 exogenously in the soil increased the activities of antioxidants like SOD, CAT, POD, and APX by 64, 24, 36, and 84% in the roots and also increased by 21, 12, 23, and 60% in the leaves of 40 day radish while also increased by 42, 13, 18, and 60% in the roots and also increased by 13, 14, 16, and 41% in the leaves in Mino radish, respectively, in comparison to those plants grown without KNO3. We found that KNO3 substantially improved plant growth by lowering the levels of oxidative stress biomarkers, thereby further stimulating the antioxidant potential system, which led to an improved nutritional profile of both R. sativus L. genotypes under normal and stressed conditions. The current study would offer a deep theoretical foundation for clarifying the physiological and biochemical mechanisms by which the KNO3 improves salt tolerance in R. sativus L. genotypes.

Biogenic silicon nanoparticles mitigate cadmium (Cd) toxicity in rapeseed (Brassica napus L.) by modulating the cellular oxidative stress metabolism and reducing Cd translocation.

Nano-enabled strategies have emerged as promising alternatives to resolve heavy metals (HMs) related harms in an eco-friendly manner. Here, we explored the potential of biogenic silicon nanoparticles (SiNPs) in alleviating cadmium (Cd) stress in rapeseed (Brassica napus L.) plants by modulating cellular oxidative repair mechanisms. Biogenic SiNPs of spherical shapes with size ranging between 14 nm and 35 nm were synthesized using rice straw extract and characterized through advanced characterization techniques. A greenhouse experiment results showed that SiNPs treatment at 250 mg kg-1 significantly improved growth parameters, including fresh weight (33.3 %) and dry weight (32.6 %) of rapeseed plants than Cd-treated control group. Photosynthesis and leaf gas exchange parameters were also positively influenced by SiNPs treatment, indicating enhanced photosynthetic efficiency. Additionally, SiNPs treatment at 250 mg kg-1 increased the activities of antioxidant enzymes such as superoxide dismutase (19.1 %), peroxidase (33.4 %), catalase (14.4 %), and ascorbate peroxidase (33.8 %), which may play a crucial role in ROS scavenging and reduction in Cd-induced oxidative stress. TEM analysis revealed that SiNPs treatment effectively mitigated Cd-induced damage to leaf ultrastructure, while qPCR analysis showed that SiNPs treatment changed the expressions of the antioxidant defense and stress related genes. Moreover, SiNPs treatment significantly influenced the Cd accumulation and Si contents in plants. Overall, our findings revealed that biogenic SiNPs have great potential to serve as a sustainable, eco-friendly, and non-toxic alternative for the remediation of Cd toxicity in rapeseed plants.

Evaluation of nanoceria on cadmium uptake in Triticum aestivum (L.) and its implications for dietary health risk.

In recent times, significant attention has been directed toward the synthesis and application of nanoparticles (NPs) in agriculture sector. In current study, nanoceria (CeO2 NPs) synthesized by green method were employed to address cadmium (Cd) accumulation in wheat (Triticum aestivum L.) cultivated in field with excess Cd. The application of CeO2 NPs was carried out through foliar spraying, performed twice during the growth of T. aestivum. Four levels of CeO2 NPs were used: T0, T1, T2, and T3 as 0, 50, 75, and 100 mgL-1, respectively. Results highlighted the positive effects of CeO2 NPs on various growth parameters, including plant height, spike length, photosynthetic related attributes, as well as straw and grain of grains in comparison to T1 (control group). Furthermore, CeO2 NPs led to a reduction in oxidative stress in the leaves and enhanced in enzyme activities in comparison to T1. Notably, Cd concentrations in straw, roots, and grains exhibited a decline following the treatment with CeO2 NPs, in contrast to the control group. In terms of health implications, the calculated health risk index associated with dietary consumption of grains by adults remained below the defined threshold with supply of nanoparticles. Foliar application of CeO2 NPs proved to be an effective approach in reducing cadmium content in wheat grains. This reduction holds significant potential for minimizing the risk of cadmium exposure to human health through the food chain. Employing the green synthesis method amplifies the potential for extensive production and a wide array of environmental applications for CeO2 NPs. This dual capacity makes them proficient in tackling environmental stresses while concurrently mitigating adverse ecological effects.

The effect of gibberellic acid on wheat growth and nutrient uptake under combined stress of cerium, zinc and titanium dioxide nanoparticles.

Nanoparticles (NPs) are released and dispersed in the environment because of increased manufacturing and use of nano products. NPs disturb the growth of plants depending upon types, exposure duration and plant species. The purpose of this research was to explore the role of gibberellic acid (GA) exposure through foliar route on wheat growth under alone or combined soil application of cerium oxide (CeO2), zinc oxide (ZnO), and titanium dioxide (TiO2) NPs. GA was foliar-applied (200 mg/L) on the wheat plants treated with individual and in all possible combination of the selected NPs. Explorations have revealed that the combination of NPs and GA worked well to enhance the plant growth and selected nutrient status than NPs alone. Furthermore, GA decreased the boosted antioxidant enzyme activities under the combination and individual NPs compared to the alone NPs treated plants, lowered the oxidative stress in wheat plants which provided the additional proof that GA decreased oxidative damage in plants. Combined NPs showed differential effects than individual NPs application irrespective of GA exposure which varied with NPs combination and studied parameters of plants. GA + NPs differentially affected the potassium, phosphorus, iron and manganese concentrations in wheat tissues than NPs alone treatments. Overall, GA can be applied when there is excess of NPs (either alone or in combination) in the growth medium to ensure the growth of crops. However, further studied are needed with other plant species and alone or combined use of different NPs under GA treatment before any final recommendation.