RESUMO
The Equatorial South Atlantic region, spanning over 1700 km, is currently undergoing extensive exploitation through various activities such as oil extraction, desalination plants, marine mineral explorations, and wind power for green hydrogen production. This undoubtedly also contributes to the exacerbation of pre-existing chronic environmental impacts. This study aims to investigate the concentrations of 60 substances, categorized as Persistent Organic Pollutants (POPs) and Contaminants of Emerging Concern (CECs) from various classes including: polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), organochlorine pesticides (OCPs), as well as Pyrethroids (PPs), Triazines (TPs) and Organophosphates (OPPs) pesticides in consumable fish, shellfish, and crabs. The bivalve (Mytella charruana), crab (Ucides cordatus), and catfish (Sciades herzbergii) samples were collected in areas of ecological, environmental and economic importance. This data was used to estimate concentrations in the organisms, and to calculate cancer and human health risk. The most prevalent pollutant classes in the organisms were OCPs, followed by TPs and PPs. Shellfish and fish samples had more compounds indicating health risks, when compared to crabs. The substances causing cancer risks varied across organisms and study areas. The heightened cancer risks linked to specific compounds in various species highlight the urgent need to address persistent pollutants to prevent long-term health impacts on both humans and wildlife. Compounds such as PPs, TPs, and OPPs pose significant risks of neurotoxicity and endocrine disruption. This study underscores the interconnectedness of environmental and human health in coastal ecosystems, calling for continuous monitoring and adaptive management strategies to protect these fragile environments and the communities that rely on them.
Assuntos
Organismos Aquáticos , Animais , Neoplasias , Poluentes Químicos da Água/análise , Bifenilos Policlorados/análise , Praguicidas/análise , Monitoramento Ambiental , Éteres Difenil Halogenados/análise , Humanos , Peixes , Hidrocarbonetos Clorados/análise , BraquiúrosRESUMO
The Jundiaí-Potengi Estuary (EJP) is located on the semi-arid coast of northeastern Brazil and is influenced by multiple sources of contamination. The sediment quality of EJP was assessed by using a multi-geochemical approach during the dry and wet seasons. Sediments were analyzed for concentrations of nutrients, metals, polycyclic aromatic hydrocarbons (PAHs), pesticides, hormones (natural and synthetic), and sterols. The results were integrated by multivariate methods. The sediment was altered by the presence of contaminants from anthropogenic and natural sources. The middle and lower estuarine areas were considered more degraded in both seasons. In these regions, metals, hormones, sterols, and PAHs were found, indicating that these regions are severely affected by industrial, sanitary and aquaculture effluents, combustion of fossil fuels, and oil spills. The upstream region was contaminated by pesticides. The contamination pattern along the EJP shows the prevalence of local sources which continuously release the chemicals into the estuary. Worse conditions occurred during the rainy season, when the runoff from urban and rural areas is more intense and carries a greater load of contaminants to the EJP.
Assuntos
Praguicidas , Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Brasil , Monitoramento Ambiental/métodos , Estuários , Sedimentos Geológicos , Hormônios , Metais/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Esteróis , Poluentes Químicos da Água/análiseRESUMO
This investigation evaluated the bioaccumulation potential of the tropical estuarine bivalve Anomalocardia flexuosa for trace metals. To this aim, chemical and sedimentological analyses and bioaccumulation tests were performed. The sediments were mainly composed by fine-sands and mud, with variable levels of organic matter and CaCO3. Muddy sediments from a depositional site (P2) presented the highest concentrations of metals, despite SEM/AVS not indicating bioavailability. Bioaccumulation factors showed high ratios for Cd, Ni, and Zn, while associations between the contents of mud, organic matter, CaCO3 and metals in sediments and tissues of A. flexuosa were indicated by a principal component analysis. The SEM/AVS was not effective to predict the bioavailability through dissolved metals. The results showed that contaminants were bioavailable, while the performed bioaccumulation test proved to be a reliable technique for assessing sediment contamination in estuarine regions. Moreover, A. flexuosa was considered an adequate test organism for bioaccumulation studies.
Assuntos
Bivalves , Metais Pesados , Poluentes Químicos da Água , Animais , Bioacumulação , Monitoramento Ambiental , Sedimentos Geológicos , Metais/análise , Metais Pesados/análise , Poluentes Químicos da Água/análiseRESUMO
The environmental impact of microplastics is a challenging theme, especially under realistic experimental conditions. We investigated physiological responses to 0.1-1.0 µm PVC particles intake by the mussel Perna perna after a relative long-term exposure (90 days) at a less extreme concentration compared with previous studies (0.125 g/L). Microplastic intake was inferred by the presence of PVC in the feces of mussels, and physiological damages were assessed through ingestion rate, assimilation efficiency, growth rate, cellular and molecular biomarkers (lysosomal integrity, lipid peroxidation, and DNA damage), and condition index. All physiological responses showed nonsignificant effects of the microplastics on the exposed mussels. We suggest that, despite the experimental concentration of microplastics, mussels were able to acclimate to the exposure through their abilities for long-term recovery and tolerance to stresses. These data have positive implications for environmental health and in terms of human food resource because mussel farming is a worldwide practice that heavily relies on plastic materials, increasing the chances of microplastic exposure and mussels contamination.
Assuntos
Biomarcadores Ambientais/efeitos dos fármacos , Perna (Organismo)/efeitos dos fármacos , Plásticos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Dano ao DNA/efeitos dos fármacos , Ecotoxicologia , Peroxidação de Lipídeos/efeitos dos fármacos , Perna (Organismo)/fisiologia , Cloreto de Polivinila/toxicidadeRESUMO
The Ribeira de Iguape River (Southeast Brazil) is metal contaminated by mining activities. Despite it has been cataloged as "in via of restoration" by the literature, this basin is still a sink of pollution in some segments of the fluvial system. This study aimed to assess the sediment quality in the lower part of the RIR basin. The employed approach was based on biological responses of the freshwater clam Corbicula fluminea after 7-day exposure bioassays using as the reference site the Perequê Ecological Park. Toxic responses (burial activity and lethality) and biochemical biomarkers (GST, GR, GPx, LPO, MTs, AChE and DNA damage) were evaluated and then integrated with metal bioavailability and chemical concentrations to address the sediment quality in the area through the weight-of-evidence approach. A multivariate analysis identified linkages between biological responses and contamination. Results pointed that, despite being below the benchmarks of the US Environmental Protection Agency, there is slight metal contamination in the lower part of the basin which induces oxidative stress in C. fluminea; other toxic responses were sometimes attributed to As and Cr bioaccumulation. The sediment quality values (TEL-PEL values in mg/kg) were calculated for the current study for As (0.63-1.31), Cr (3.5-11.05), Cs (1.0-1.17), Cu (6.32-7.32), Ni (6.78-7.46), Ti (42.0-215), V (1.77-8.00). By comparison with other international guidelines, the sediment quality of the lower basin of the Vale de Ribeira does not identify a significant environmental risk.
Assuntos
Sedimentos Geológicos/química , Mineração , Poluentes Químicos da Água/metabolismo , Animais , Biomarcadores/metabolismo , Bivalves/enzimologia , Bivalves/metabolismo , Brasil , Água Doce , Estresse Oxidativo , Poluentes Químicos da Água/toxicidadeRESUMO
Tropical marine environments are rich in biodiversity and the presence of harbor activities in these areas can harm the coastal ecosystems. In this study, we assessed sediment quality of two harbors from a tropical region in Brazil by applying multiple lines-of-evidence approach. This approach included the integration of results on: (1) grain size, organic matter, organic carbon, nitrogen, phosphorus, trace metals, polycyclic aromatic hydrocarbons, linear alkylbenzenes, and tributyltin; (2) acute toxicity of whole sediments and chronic toxicity of liquid phases; and (3) benthic community descriptors. Our results revealed that the main contaminants detected in sediments from Mucuripe and Pecém Harbors were chromium, copper, nitrogen, zinc, and tributyltin. These toxicants arise from typical harbor activities. However, the changes in benthic composition and structure appear to depend on a combination of physical impacts, such as the deposition of fine sediments and the toxic potential of contaminants, especially in Mucuripe. Thus, apart from toxicants physical processes are important in describing risks. This information may assist in management and conservation of marine coastal areas.
Assuntos
Sedimentos Geológicos/análise , Navios , Poluentes Químicos da Água/análise , Derivados de Benzeno/análise , Brasil , Monitoramento Ambiental , Metais/análise , Nitrogênio/análise , Fósforo/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Compostos de Trialquitina/análise , Clima TropicalRESUMO
The sediment quality of Ribeira de Iguape River is affected by former Pb extraction mining. Some studies affirm the restoration status of the basin, however, mobility of metals and its associated risk is still questioned. This study integrates the metal concentrations in the lower part of the basin with different contamination source to determine the existence of risks associated with the mobile fractions of the geochemical matrix. Despite concentrations of metals were low and the environmental risk factor values were negative, our results indicated that As, Mn, Pb, and V were present in the most labile forms. The multivariate analysis conducted using metal concentrations, environmental risk factor values and speciation suggested that any risk would be associated with the labile fractions of the analyzed elements, especially for Pb. The station from Registro was stressed by Co, Pb and Zn; with Pb under the reactive fraction that could be associated with high mobility and potential bioavailability.
Assuntos
Monitoramento Ambiental , Sedimentos Geológicos/química , Metais Pesados/análise , Poluentes Químicos da Água/análise , Brasil , Fracionamento Químico , Sedimentos Geológicos/análise , Mineração , RiosRESUMO
This study evaluated the alteration of the enzymatic system of the freshwater Asian clam exposed to different copper concentrations. Individuals of Corbicula fluminea were exposed to different concentrations of dissolved Cu (0.5, 1, and 2 mg L(-1)) for 7 days, then, biomarkers of oxidative stress (GST, GPx, GR), exposure (MTs), effect (AChE), and damage (LPO, DNA strand breaks) were quantified. Results showed positive correlations between dissolved metal concentrations and GPx, MTs, and DNA damage, and negative correlation with GST and AChE. In contrast, no clear trend was found for GR and LPO. In general, the established mechanisms of protection might have a beneficial effect on the decreasing ROS attack on membrane and the activation of the metallothioneins. Integrated biomarker analysis revealed that the measured alterations are well correlated with the levels of increasing dissolved copper concentrations in water, demonstrating the effectiveness of this organism for biomonitoring approach purposes.
Assuntos
Cobre/toxicidade , Corbicula/metabolismo , Substâncias Perigosas/toxicidade , Testes de Toxicidade , Animais , Biomarcadores/metabolismo , Corbicula/efeitos dos fármacosRESUMO
The biomonitoring of fish using biomarkers represents a useful tool for the assessment of aquatic pollution. This study evaluated the sublethal toxic effects of aquatic pollution on fish collected from a site contaminated by metals. Water and fish (Oreochromis niloticus) samples were collected from a pond in the Parque Ecológico do Tietê (PET) that lies along the Tietê River (São Paulo, Brazil), and from a control site (an experimental fish farm). The metal content of the water was evaluated, and fish were used to examine the properties of gill mucus and blood. The PET fish were evaluated for alterations in the in vitro transportability of mucus and changes in blood properties (e.g., cell volume, hemoglobin concentration, red blood cells, and white blood cell count). The results of the water analyzes indicated metal levels above the legal standards for Fe (0.71 mg/L), Ni (0.06 mg/L), Mn (0.11 mg/L), and Pb (0.48 mg/L). Compared to the controls, the hematologic parameter analyzes of PET fish revealed significantly higher numbers of erythrocytes (RBC), leukocytes (WBC), lymphocytes, erythroblasts, and Mean Corpuscular Volume (MCV); however, the hemoglobin content and Mean Corpuscular Hemoglobin Concentration (MCHC) values were significantly lower. The frequencies of nuclear abnormalities and micronuclei were significantly higher and the mucociliary transport was significantly lower in PET fish than in the controls. These results suggest that fish from the contaminated site exhibit a series of physiological responses, which probably indicate health disturbances. Furthermore, the results suggest that blood and mucus are promising, non-destructive targets for use in the monitoring of pollution.
Assuntos
Ciclídeos/metabolismo , Metais/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Biomarcadores/sangue , Biomarcadores/metabolismo , Brasil , Monitoramento Ambiental , Testes Hematológicos , Metais/análise , Metais/metabolismo , Muco/efeitos dos fármacos , Muco/metabolismo , Lagoas/química , Poluentes Químicos da Água/sangue , Poluentes Químicos da Água/metabolismoRESUMO
This study focuses on the discussion of different lines of evidence (LoEs) applied to a sediment-quality assessment that considered the following: chemical concentrations of metals; polycyclic aromatic hydrocarbons (PAHs) in estuarine waters, sediments, and oysters (native and caged Crassostrea brasiliana); PAHs in semipermeable membrane devices (SPMDs); simultaneously extracted metals-acid volatile sulfides (SEM-AVS); benthic community assessment (the exploratory benthic index and the relative benthic index); chronic toxicity tests with the sea urchin Lytechinus variegatus; and bioaccumulation models. Significantly contaminated sediments from the Santos Estuarine System and the consequent toxicity of tested organisms were measured. Caged oysters presented bioaccumulation rates ≤2,500% of total PAH content and 200% of metal content when compared with control organisms from an uncontaminated area. SPMD results presented the same bioaccumulation pattern as caged oysters but at lower concentrations. Benthic communities presented some alterations, and there was a predominance of tolerant species in the inner part of the estuary. According to the SEM-AVS approach, metals should be assumed to be nonbioavailable, but experiments with transplanted C. brasiliana showed metal bioaccumulation, particularly in the cases of chromium, copper, mercury, and zinc. The weight-of-evidence approach was applied to compare and harmonize LoEs commonly used in sediment-quality assessments and to then classify estuary environments according to both their potential for having adverse effects on the biota and their possible ecological risks. All of the results of these approaches (except for SEM-AVS) were found to complement each other.
Assuntos
Monitoramento Ambiental/métodos , Estuários , Poluentes Químicos da Água/análise , Animais , Brasil , Sedimentos Geológicos/química , Metais/análise , Metais/metabolismo , Ostreidae/metabolismo , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Rios/química , Poluentes Químicos da Água/metabolismoRESUMO
In order to evaluate if sediment metal contamination is responsible for benthic degradation and identify possible reference sites in Todos os Santos Bay (TSB), comparisons between a highly impacted (Subaé) and less impacted (Jaguaripe) estuarine systems were made based on (i) field assessment of macrobenthic assemblage, (ii) sediment metal concentrations and (iii) chronic toxicity test with the tropical copepod Nitokra sp. Data were integrated by multivariate analysis (BIOENV and PCA) and the ratio-to-mean (RTMe) approach. Estuaries were divided into four different salinity zones to avoid misclassification of benthic conditions. Salinity was the main variable correlated to the benthic distribution in both estuaries, indicating that categories based on salinity features seem to be suitable in TSB. Correspondence among lines of evidence differed in low and high metal contaminated systems. Chronic toxicity was found along both the entire systems, being considerably higher in Jaguaripe. However, there was no clear evidence of metal contamination and benthic alteration in most stations of Jaguaripe. Although the concentrations of Sr and Cu were correlated to the benthic assemblage in Jaguaripe, it is unlikely that toxicity has been caused by these elements. The benthic assemblage distribution of Jaguaripe seems to be rather related to natural stressful conditions of transitional waters. Even though the Jaguaripe estuary might not be pristine, it can be used as a reference estuary for benthic assessment in TSB. Regarding the Subaé estuary, toxicity and Zn were also correlated to the benthic assemblage and most stations showed signs of benthic alteration and metal contamination. All lines of evidence were in agreement providing evidences that metal contamination might be responsible for benthic degradation in Subaé.
Assuntos
Monitoramento Ambiental , Estuários , Sedimentos Geológicos/química , Metais/análise , Clima Tropical , Poluentes Químicos da Água/análise , Animais , Invertebrados/efeitos dos fármacos , Metais/toxicidade , Análise Multivariada , Salinidade , Poluentes Químicos da Água/toxicidadeRESUMO
Wet deposition is an important process that removes pollutants from the atmosphere and transfers them to waters and soil. The goal of this study was to assess the biological effects of the atmospheric contamination of rainwater in the metropolitan area of São Paulo (MASP) using Daphnia similis, Ceriodaphnia dubia, and Vibrio fischeri. Experimental assays were carried out according to standard toxicity methodology. Twenty-three rainwater samples were collected from October 2007 to December 2008, at the Nuclear Research Institute (IPEN), in MASP. Major ions were determined by ionic chromatography, which showed NH4(+) and NO3(-) as prevalent ions. Ecotoxicological results confirmed toxic potential of rainwater, as all samples were toxic to D. similis and C. dubia. The V. fischeri luminescence reduction confirmed those negative effects of rainwater and percentage inhibition of relative luminescence ranged from 0.2 to 0.9 for 16 samples. Worse conditions were observed during the rainy season, suggesting convective rains are more effective in transferring contaminants and toxicity from atmosphere to surface.
Assuntos
Monitoramento Ambiental , Chuva/química , Poluentes Químicos da Água/toxicidade , Aliivibrio fischeri , Animais , Brasil , Cladocera , Daphnia , Poluentes Químicos da Água/análiseRESUMO
This study aimed to evaluate the sediment quality in the estuarine protected area known as Cananéia-Iguape-Peruíbe (CIP-PA), located on the southeastern coast of Brazil. The study was designed considering possible negative effects induced by the city of Cananéia on the sediment quality of surrounding areas. This evaluation was performed using chemical and ecotoxicological analyses. Sediments were predominantly sandy, with low CaCO3 contents. Amounts of organic matter varied, but higher contents occurred closer to the city, as well as did Fe and Total Recoverable Oils and Greases (TROGs) concentrations. Contamination by Cd and Cu was revealed in some samples, while concentrations of Zn were considered low. Chronic toxicity was detected in all tested sediments and acute toxicity occurred only in sediments collected near the city. The principal component analysis (PCA) revealed an association among Cd, Cu, Fe, TROG, fines, organic matter, CaCO3, and chronic toxicity, whereas acute toxicity was found to be associated with Zn and mud. However, because Zn levels were low, acute toxicity was likely due to a contaminant that was not measured. Results show that there is a broad area within the CIP-PA that is under the influence of mining activities (chronic toxicity, moderate contamination by metals), whereas poorer conditions occur closer to Cananéia (acute toxicity); thus, the urban area seems to constitute a relevant source of contaminants for the estuarine complex. These results show that contamination is already capable of producing risks for the local aquatic biota, which suggests that the CIP-PA effectiveness in protecting estuarine biota may be threatened.
Assuntos
Cidades , Monitoramento Ambiental/métodos , Estuários , Mineração , Poluentes Químicos da Água/análise , Brasil , Conservação dos Recursos Naturais , Ecotoxicologia , Sedimentos Geológicos/química , Metais/análiseRESUMO
Cigarette butts (CBs) are the most common type of beach litter worldwide and contain a complex mixture of chemicals. Given the recent interest in this emerging problem, it is important to assess the toxicity of CB leachates to a range of species from different regions, sensitivities, and ecological traits. We evaluated the waterborne toxicity of smoked CB to tropical invertebrates. Leachates were prepared in the laboratory and characterized for trace elements (Mn, Fe, Co, Ni, Cu, Zn, As, Cd, and Pb), ammonia nitrogen, and polycyclic aromatic hydrocarbons. Then a set of toxicity tests with marine invertebrates was performed as follows: the brine shrimp Artemia sp. (nontoxic); the amphipod Tiburonella viscana (median lethal concentration [LC50] of 0.038 CB/L); the tanaid Monokalliapseudes schubarti (LC50 of 0.126 CB/L); the copepods Tisbe biminiensis (median effect concentration [EC50] of 0.038 CB/L) and Nitokra sp. (EC50 of 0.009 CB/L); pluteus stage larvae of the sea urchin Echinometra lucunter (EC50 of 0.152 CB/L); the sand dollar Mellita quinquiesperforata (EC50 of 0.054 CB/L); and D-stage larvae of the mussel Perna perna (EC50 of 0.005 CB/L). The predicted no-effect concentration was estimated using species sensitivity distribution, producing a 5th percentile hazard concentration of 0.015 CB/L. This preliminary threshold allowed us to estimate the potential impact of a single CB to 67 L of seawater via leaching, contributing to the advancement of knowledge regarding the contamination, toxicity, and ecological risks of cigarette waste. Environ Toxicol Chem 2024;43:374-384. © 2023 SETAC.
Assuntos
Invertebrados , Produtos do Tabaco , Animais , Produtos do Tabaco/toxicidade , Organismos Aquáticos , Testes de Toxicidade , LarvaRESUMO
In 2015, > 460,000 L of aqueous film-forming foam (AFFF) and fire suppressors containing per- and polyfluoroalkyl substances (PFAS) were used to combat a fire at a petrochemical fuel storage terminal in the Port of Santos (Brazil). Sediments from seven sites were sampled repeatedly from 2 weeks to 1 year after the fire (n = 30). Æ©15PFAS concentrations ranged from 115 to 15,931 pg g-1 dry weight (dw). Perfluorooctane sulfonic acid (PFOS) was the most frequently detected compound with concentrations ranging from 363 to 4517 (average = 1603) pg g-1dw to <47.1 to 642 (average = 401) pg g-1 dw, followed by perfluorohexanoic acid (PFHxA) (from 38.8 to 219 (average = 162) pg g-1 dw after 15 days and from <20.8 to 161 (average = 101) pg g-1 dw one year later). Together, the hydrodynamics and fire events documented in the region were important features explaining the spread of PFAS.
Assuntos
Ácidos Alcanossulfônicos , Monitoramento Ambiental , Fluorocarbonos , Poluentes Químicos da Água , Fluorocarbonos/análise , Poluentes Químicos da Água/análise , Ácidos Alcanossulfônicos/análise , Brasil , Sedimentos Geológicos/química , Caproatos/análiseRESUMO
Microplastics and nanomaterials are applied in a myriad of commercial and industrial applications. When leaked to natural environments, such small particles might threaten living organisms' health, particularly when considering their potential combination that remains poorly investigated. This study investigated the physiological and biochemical effects of polyethylene (PE; 64-125 µm in size, 0.1, 1.0, and 10.0 mg·L-1) single and combined with an engineered nanomaterial applied in antifouling coatings, the copper-aluminium layered double hydroxides (Cu-Al LDH; 0.33, 1.0, and 3.33 mg·L-1) in the flatfish Solea senegalensis larvae (8 dph) after 3 h exposure, in a full factorial design. Particles ingestion, histopathology, and biochemical biomarkers were assessed. Fish larvae presented <1 PE particles in their gut, independently of their concentration in the medium. The histological health index showed minimal pathological alterations at PE combined exposure, with a higher value observed at 1 mg LDH·L-1 × 0.1 mg PE·L-1. Gut deformity and increased antioxidant defences (catalase), neurotransmission (acetylcholinesterase), and aerobic energy production (electron transport system) were observed at PE ≥ 1.0 mg·L-1. No oxidative damage (lipid peroxidation) or alterations in the detoxification capacity (glutathione-S-transferase) was observed on single and combined exposures. PE, combined or not with Cu-Al LDH, does not seem to compromise larvae's homeostasis considering levels reported so far in the marine and aquaculture environments. However, harsh effects are expected with MP contamination rise, as projections suggest.
Assuntos
Linguados , Poluentes Químicos da Água , Acetilcolinesterase , Animais , Microplásticos , Plásticos , Polietileno/toxicidade , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
The increasing consumption of anticancer drugs through single and/or combinatory chemotherapy worldwide raised concern regarding their toxicity burden in coastal zones. The toxicity of a mixture of three compounds involving the drugs cisplatin (CisPt), cyclophosphamide (CP) and tamoxifen (TAM) was determined on the marine polychaete Nereis diversicolor exposed to an increasing range of their concentrations, respectively: Mix A: 0.1 + 10 + 0.1 ng L-1; Mix B: 10 + 100 + 10 ng L-1; Mix C: 100 + 500 + 25 ng L-1; Mix D: 100 + 1000 + 100 ng L-1. Different endpoints were assessed, including disturbance in the burrowing behaviour, neurotoxicity (acetylcholinesterase - AChE activity), antioxidant enzymes (superoxide dismutase - SOD; catalase - CAT; selenium-dependent glutathione peroxidase - Se-GPx and total glutathione peroxidases T-GPx activities), biotransformation metabolism (glutathione-S-transferases - GST), lipid peroxidation (LPO) and genotoxicity (DNA damage). Biological effects of the mixtures of anticancer compounds on N. diversicolor were compared with previous studies about effects on the same biological model under single-drug exposure conducted with the same molecules. Regarding SOD activity, TAM showed an antagonist effect over CisPt and CP in mixtures C and D. In Mix D, there was a synergistic effect of TAM and CisPt that inhibited CAT activity and an additive interaction of CisPt and CP on the Phase II biotransformation enzyme. Drugs in Mix A also suppressed polychaetes' GST activity, although different from the respective single-drug responses, besides able to induce T-GPx activity, that was not sufficient to avoid oxidative damage and mid-grade DNA damage. Due to the absence of burrowing impairment in Mix A, mechanisms involved in neurotoxicity were other than the one driven by AChE alterations. At the intermediary concentrations (Mix B and C), only LPO occurred. Data from drugs individually may not predict the risks provided by mixtures.
Assuntos
Antineoplásicos/toxicidade , Poliquetos/fisiologia , Poluentes Químicos da Água/toxicidade , Acetilcolinesterase/metabolismo , Animais , Antineoplásicos/metabolismo , Antioxidantes/metabolismo , Biotransformação , Catalase/metabolismo , Ciclofosfamida/metabolismo , Dano ao DNA , Glutationa Peroxidase/metabolismo , Glutationa Transferase/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Estresse Oxidativo/fisiologia , Poliquetos/metabolismo , Selênio/metabolismo , Superóxido Dismutase/metabolismoRESUMO
In this study, the performance of two native bivalves in responding to sediment resuspension was investigated during dredging operations of a semi-arid bay (Mucuripe, NE Brazil). The clam Anomalocardia flexuosa and the oyster Crassostrea rhizophorae were selected and caged in two sites influenced by the dredging plume. We assessed the bioaccumulation of metals and hydrocarbons in both species as biomarkers of exposure and then, biomarkers' responses were assessed in gills and digestive glands over a 28 days period, at 7 days intervals: the activities of phase I and II, and antioxidant enzymes, levels of lipid peroxidation and DNA strand breaks. Both transplanted bivalves accumulated Cu, polycyclic aromatic hydrocarbons (PAHs), and linear alkylbenzenes (LABs) in their whole-body tissues, whereas Ni, Pb and Zn accumulation was species-dependent. The exposure time set at day 28 was considered appropriate. Biomarkers exhibited time-related responses in both species, but gills exhibited a more sensitive response, indicating a function of barrier against the uptake of chemicals and also a relevant tissue to be targeted. In clams, Phase I and II enzymes (ethoxyresorufin O-deethylase and glutathione S-transferase) were induced during the period of intense dredging, while in oysters they were activated at the end of operations. Induction of antioxidant enzymes (glutathione peroxidase and glutathione reductase) and elevated levels DNA damage were observed in both exposure surveys. Clams and oysters were sensitive and responded to the exposure of resuspended sediments and the biomarkers of effects were associated with bioaccumulation of contaminants in the integrated analysis. These results indicate that The clam A. flexuosa and the oyster C. rhizophorae are suitable models to be used in monitoring programs or field exposure experiments in tropical regions.
Assuntos
Bivalves , Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Animais , Biomarcadores , Bivalves/química , Bivalves/genética , Bivalves/metabolismo , Brasil , Dano ao DNA , Monitoramento Ambiental , Glutationa TransferaseRESUMO
Anthropogenic molecular markers were used to assess chemicals inputs and ecological risks associated from multiple sources to sediments in one of the largest tropical mangrove forests of South America, with a particular focus on lesser studied compounds resulting from rural activities. Total concentrations ranged from 23.4 to 228.2â¯ngâ¯g-1 for polycyclic aromatic hydrocarbons (∑PAHs), 750.4 to 5912.5â¯ngâ¯g-1 for aliphatic hydrocarbons (∑AHs), 32.4 to 696.6â¯ngâ¯g-1 for pesticides (∑pesticides), 23.1 to 2109.7â¯ngâ¯g-1 for coprostanol and sterols (∑sterols), 139.3 to 580.2â¯ngâ¯g-1 for naturals hormones (∑natural hormones) and 334.1 to 823.4â¯ngâ¯g-1 for synthetics hormones (∑synthetic hormones). The PAHs and AHs used as traditional anthropogenic markers showed a mixture between natural and anthropogenic sources, related mainly to inputs from higher plants, phytoplankton and both, biomass and petroleum combustion. Rural activities linked to agricultural pest control are the predominant source of pesticides, although minor inputs from pesticides used in urban public health campaigns and household activities were also detected. Synthetic hormones levels are two to three orders of magnitude greater than natural hormones levels and no correlations were observed between the main sewage markers and synthetic hormone concentrations, rural activities such as animal husbandry, which use drugs in management, may be the predominant anthropogenic sources of these compounds in the region. Traditional markers failed to detect ecological risks in rural areas, where synthetic substances (e.g. pesticides and hormones) are widely used and introduced in the environment.
Assuntos
Monitoramento Ambiental , Sedimentos Geológicos/análise , Poluentes Químicos da Água/análise , Áreas Alagadas , Brasil , Sedimentos Geológicos/química , Parques Recreativos , Medição de RiscoRESUMO
Submarine sewage outfalls (SSOs) are considered the main input source of contaminants of emerging concern continuously released in coastal areas, with the potential to cause adverse effects for aquatic organisms. This work presents the investigation of nine endocrine disrupting chemicals (EDCs) and 26 pharmaceutically active chemicals (PhACs) in marine sediments within the vicinities of 7 SSOs along the São Paulo State Coast (Brazil). Method optimization for the multi-residue determination by GC-MS/MS and LC-MS/MS using QuEChERS extraction/clean-up are discussed. Results demonstrate the occurrence of EDCs in concentrations ranging from less than method quantification limits (MQL) to 72.5â¯ngâ¯g-1 in sediments. All PhACs were Assuntos
Disruptores Endócrinos/análise
, Monitoramento Ambiental/métodos
, Sedimentos Geológicos/química
, Esgotos/química
, Navios
, Poluentes Químicos da Água/análise
, Organismos Aquáticos/efeitos dos fármacos
, Brasil
, Cromatografia Líquida
, Medição de Risco
, Espectrometria de Massas em Tandem