RESUMO
The activator protein for the enzymatic hydrolysis of sulfatide, ganglioside GM1, and globotriaosylceramide was purified from human kidney, brain, and urine. As far as they could be assayed, these three activities cochromatographed during all steps, indicating that they are due to the same protein. This result was corroborated by immunochemical comparison of individually purified activator preparations. In contrast, the activator for ganglioside GM2 hydrolysis could clearly be separated from the other activities. Kinetic data were determined for the interaction of the sulfatide activator with the different glycolipids and hydrolases.
Assuntos
Gangliosídeo G(M1)/metabolismo , Globosídeos/metabolismo , Glicoesfingolipídeos/isolamento & purificação , Glicoesfingolipídeos/metabolismo , Sulfoglicoesfingolipídeos/metabolismo , Triexosilceramidas , Química Encefálica , Cromatografia DEAE-Celulose , Cromatografia Líquida de Alta Pressão , Ativação Enzimática , Globosídeos/urina , Humanos , Hidrólise , Imunoeletroforese Bidimensional , Focalização Isoelétrica , Rim/análiseRESUMO
THE LIPID pattern of blood and liver of mice infected with S. mansoni as well as in male and female worms was studied. Experiments were also carried out on non-infected and infected mice after treatment with Niridazole. It was found that Niridazole caused a significant increase in liver triglycerides and serum free acids. These changes were more pronounced in non-infected animals than in infected ones. The drug was found to reduce worm phospholipids and to increase their triglyceride level. The male worms were more affected than the female ones.