RESUMO
Testing for specific antibodies against Bovine viral diarrhea virus (BVDV) in pooled serum may present an opportunity to decrease the cost of screening for herds of high seroprevalence and increased likelihood of active infection. Experimental serum pools (n = 280) were created by combining equal aliquots of serum from between 5 and 25 individuals. A further 188 serum pools were generated from field serum samples. All pools and individual sera were tested for BVDV-specific antibodies by enzyme-linked immunosorbent assay (ELISA), according to manufacturer's instructions. Pools returned repeatable results, with coefficients of variation generally below 10%. The presence of serum from a persistently infected (PI) individual in the pool had no significant effect on the ELISA sample-to-positive (S/P) ratio. The results revealed that a single strong antibody-positive individual could maintain a positive result (at the manufacturer's threshold) in pools of up to 128, while even a single weak-positive animal would generate a positive result in pools of up to 8. The S/P ratio of the pool was positively related to the within-pool prevalence of antibody-positive individuals. However, as the strength of the individual positive animals contributing to the pool had a large effect on the pool S/P ratio, the S/P ratio could not be used to accurately predict the within-pool prevalence of field serum pools. An alternative method of S/P ratio interpretation was pursued, and a two-graph receiver operating characteristic analysis allowed segregation of pools into low, medium, and high risk with good results when applied to field serum pools.
RESUMO
A serological survey for Neospora caninum and Besnoitia besnoiti was carried out in beef and dairy cattle in South Australia. Serum samples of dairy cattle (n=133) from 9 properties and tank milk samples from a further 122 dairy herds were tested. An additional 810 sera from beef cattle from 51 properties were also tested. Testing at the individual animal level by IDEXX NEOSPORA X2 Ab test ELISA revealed a low prevalence of N. caninum antibodies of only 2.7% (95% CI; 1.6-3.7%) sera positive, as did the milk testing that showed 2.5% (95% CI; 1.4-3.6%) of tank milks being positive. At the herd level, 29.4% (95% CI; 16.9-41.9%) of beef, and 44.4% (95% CI; 12.0-76.9%) of dairy cattle herds showed serum antibodies. The highest within-herd prevalence in beef was 20% and 25%in dairy, which explains the low herd prevalence in dairy detected by bulk milk testing. Testing for B. besnoiti antibodies by PrioCHECK(®) Besnoitia Ab 2.0 ELISA initially identified 18.4% (95% CI: 15.8-21.0%) of 869 individual cattle sera as positive by ELISA at the manufacturer's suggested cut-off threshold (15 PP). Additional tests by immunoblot and IFAT, however, could not confirm any of the ELISA results. The use of a higher (40 PP) threshold in the ELISA is suggested to improve specificity. There is thus no evidence of B. besnoiti infection in South Australian cattle.