Identification of QTLs for symbiotic nitrogen fixation and related traits in a soybean recombinant inbred line population.

KEY MESSAGE: The genetic architecture of symbiotic N fixation and related traits was investigated in the field. QTLs were identified for percent N derived from the atmosphere, shoot [N] and C to N ratio. Soybean [Glycine max (L.) Merr.] is cultivated worldwide and is the most abundant source of plant-based protein. Symbiotic N2 fixation (SNF) in legumes such as soybean is of great importance; however, yields may still be limited by N in both high yielding and stressful environments. To better understand the genetic architecture of SNF and facilitate the development of high yielding cultivars and sustainable soybean production in stressful environments, a recombinant inbred line population consisting of 190 lines, developed from a cross between PI 442012A and PI 404199, was evaluated for N derived from the atmosphere (Ndfa), N concentration ([N]), and C to N ratio (C/N) in three environments. Significant genotype, environment and genotype × environment effects were observed for all three traits. A linkage map was constructed containing 3309 single nucleotide polymorphism (SNP) markers. QTL analysis was performed for additive effects of QTLs, QTL × environment interactions, and QTL × QTL interactions. Ten unique additive QTLs were identified across all traits and environments. Of these, two QTLs were detected for Ndfa and eight for C/N. Of the eight QTLs for C/N, four were also detected for [N]. Using QTL × environment analysis, six QTLs were detected, of which five were also identified in the additive QTL analysis. The QTL × QTL analysis identified four unique epistatic interactions. The results of this study may be used for genomic selection and introgression of favorable alleles for increased SNF, [N], and C/N via marker-assisted selection.

Shovelomics for phenotyping root architectural traits of rapeseed/canola (Brassica napus L.) and genome-wide association mapping.

Root system in plants plays an important role in mining moisture and nutrients from the soil and is positively correlated to yield in many crops including rapeseed/canola (Brassica napus L.). Substantial phenotypic diversity in root architectural traits among the B. napus growth types leads to a scope of root system improvement in breeding populations. In this study, 216 diverse genotypes were phenotyped for five different root architectural traits following shovelomics approach in the field condition during 2015 and 2016. A single nucleotide polymorphism (SNP) marker panel consisting of 30,262 SNPs was used to conduct genome-wide association study to detect marker/trait association. A total of 31 significant marker loci were identified at 0.01 percentile tail P value cutoff for different root traits. Six marker loci for soil-level taproot diameter (R1Dia), six loci for belowground taproot diameter (R2Dia), seven loci for number of primary root branches (PRB), eight loci for root angle, and eight loci for root score (RS) were detected in this study. Several markers associated with root diameters R1Dia and R2Dia were also associated with PRB and RS. Significant phenotypic correlation between these traits was observed in both environments. Therefore, taproot diameter appears to be a major determinant of the canola root system architecture and can be used as proxy for other root traits. Fifteen candidate genes related to root traits and root development were detected within 100 kbp upstream and downstream of different significant markers. The identified markers associated with different root architectural traits can be considered for marker-assisted selection for root traits in canola in future.

Predictability of intrapartum cardiotocography with meconium stained liquor and its correlation with perinatal outcome.

OBJECTIVE: To determine the relationship between the colour of liquor and the trace of cardiotocography to see whether it is reactive or non-reactive.. METHODS: This cross-sectional study was conducted at Obstetrics and Gynaecology department, Dar-ul-Sehat Hospital, Karachi from June 2015 to March 2016, and comprised women in labour who delivered singleton babies and had >37 weeks of gestation. Intrapartum monitoring by cardiotocography was conducted. The status of the amniotic membranes, colour and amount of liquor observed were recorded. Cardiotocography was performed for 30 minutes in the left lateral position on admission as well as a monitoring tool in labour at an interval of less than 4 hours. Foetal heart transducer and uterine pressure transducers were applied and the readings were recorded. SPSS 21 was used for statistical analysis. RESULTS: Of the total 200 subjects, 183(91.5%) were reactive and 17(8.5%) were non-reactive women. Overall mean age was 27.39±4.40 years. Most commonly noted risk factor were post-date 53(26.5%), anaemia 35(17.5%), premature rupture of membranes 28(14%) and pregnancy-induced hypertension 10(5%). Insignificant difference was observed in between Cardiotocography findings and risk factors of the women (p>0.05).. CONCLUSIONS: Significant change was seen in cardiotocography of clear liquor which needs more evaluation to rule out ongoing hypoxia.

Anatomical Variants Of Celiac Trunk, Hepatic And Renal Arteries In A Population Of Developing Country Using Multidetector Computed Tomography Angiography.

BACKGROUND: Multidetector Computed Tomography (MDCT)s has become a major part in evaluation of hepatic and renal tumours. With improvements in MDCT, CT angiography has also improved and normal anatomy and its variants in patients undergoing operative or interventional procedures can be effectively studied. The purpose of this study was to evaluate the frequency of anatomical variation of celiac trunk, hepatic and renal arterial systems in patients undergoing multidetector CT (MDCT) angiography of the abdominal aorta. METHODS: A descriptive, retrospective cross-sectional study was carried out on CT angiographies performed during the months of October till December 2015. Hepatic and renal arteries and celiac trunk were studied and normal and anatomical variations were noted. All patients with abnormalities affecting the vessels or a history of any vascular abnormality were excluded from the study. RESULTS: Out of total 110 patients, 69.1% had normal and 30.9% had variant hepatic artery with Michel Type IV being the most common variant whereas 88.2% had normal celiac trunk and 8.2% had gastrosplenic trunk variant. Variation in renal arterial system was observed in 15.5% of the patients with two renal arteries on right and two on left being the most common type. Multiple variants were identified in 11.8% of the patients.. CONCLUSIONS: The type and knowledge of anatomy is of prime importance for an optimum preoperative planning in surgical or radiological procedure. MDCT allows minimally invasive assessment of arterial anatomy with high quality 3D reconstruction images.

Identification of loci associated with water use efficiency and symbiotic nitrogen fixation in soybean.

Soybean (Glycine max) production is greatly affected by persistent and/or intermittent droughts in rainfed soybean-growing regions worldwide. Symbiotic N2 fixation (SNF) in soybean can also be significantly hampered even under moderate drought stress. The objective of this study was to identify genomic regions associated with shoot carbon isotope ratio (δ13C) as a surrogate measure for water use efficiency (WUE), nitrogen isotope ratio (δ15N) to assess relative SNF, N concentration ([N]), and carbon/nitrogen ratio (C/N). Genome-wide association mapping was performed with 105 genotypes and approximately 4 million single-nucleotide polymorphism markers derived from whole-genome resequencing information. A total of 11, 21, 22, and 22 genomic loci associated with δ13C, δ15N, [N], and C/N, respectively, were identified in two environments. Nine of these 76 loci were stable across environments, as they were detected in both environments. In addition to the 62 novel loci identified, 14 loci aligned with previously reported quantitative trait loci for different C and N traits related to drought, WUE, and N2 fixation in soybean. A total of 58 Glyma gene models encoding for different genes related to the four traits were identified in the vicinity of the genomic loci.

Metformin Uptake and Translocation in Chickpeas: Determination Using Liquid Chromatography-Mass Spectrometry.

Multiple chronic conditions (MCCs) such as diabetes, hypertension, heart disease, arthritis, asthma, and common respiratory problems are prevalent in over one-fourth of Americans, and separate drugs are prescribed to manage each of the diseases. The nutritive crop seeds loaded with multiple drugs could be a cheap and sustainable alternative to drugs produced by pharmaceutical companies. Our long-term goal is to produce chickpea seeds containing comparable dosages of multiple drugs regularly prescribed for managing MCC. In this work, we conducted experiments to understand the uptake and translocation of metformin into the tissues of chickpea to demonstrate the applicability of LC-HR-ToF-MS in determining metformin concentration, and to investigate responses of increased dosage of metformin and it's accumulation into the chickpea seed. We treated the chickpea plants with 100 and 500 mg/L metformin chloride and analyzed its concentration in the leaf, stem, and seeds. We observed that metformin was successfully uptaken by chickpeas plant and translocated to stem, leaf, and seeds in both treatments. We also observed that the metformin concentration is responsive and as high as 349 times increase in seed when the dosage was increased from 100 to 500 mg/L.