RESUMO
Denys-Drash syndrome is a rare human developmental disorder affecting the urogenital system and leading to renal failure, intersex disorders and Wilms' tumour. In this report, four individuals with this syndrome are described carrying germline point mutations in the Wilms' tumour suppressor gene, WT1. Three of these mutations were in the zinc finger domains of WT1. The fourth occurred within intron 9, preventing splicing at one of the alternatively chosen splice donor sites of exon 9 when assayed in vitro. These results provide genetic evidence for distinct functional roles of the WT1 isoforms in urogenital development.
Assuntos
Genes do Tumor de Wilms , Anormalidades Urogenitais , Processamento Alternativo/genética , Sequência de Bases , DNA/genética , Análise Mutacional de DNA , Éxons , Feminino , Genótipo , Células Germinativas , Humanos , Íntrons , Masculino , Dados de Sequência Molecular , Fenótipo , Síndrome , Sistema Urogenital/crescimento & desenvolvimentoRESUMO
A patient with systemic lupus erythematosus (SLE) and lupus retinopathy showed resolution of subretinal edema documented with fluorescein angiography. Subsequently at autopsy, immunofluorescence studies disclosed ocular deposition of immunoglobulins in the vascular layer of choroid capillaries and basement membranes of ciliary processes and bulbar conjunctivas. To our knowledge, these findings represent the first reported documentation of probable immune-complex ocular vasculitis in lupus retinopathy using immunofluorescent techniques, and they support the hypothesis that lupus retinopathy is caused by immune complex deposition as are other manifestations of SLE.
Assuntos
Oftalmopatias/etiologia , Olho/imunologia , Doenças do Complexo Imune/etiologia , Lúpus Eritematoso Sistêmico/imunologia , Adolescente , Corioide/imunologia , Proteínas do Sistema Complemento/análise , Túnica Conjuntiva/imunologia , Edema/etiologia , Edema/imunologia , Olho/irrigação sanguínea , Feminino , Humanos , Imunoglobulina A/isolamento & purificação , Imunoglobulina G/isolamento & purificação , Imunoglobulina M/isolamento & purificação , Properdina/análise , Doenças Retinianas/etiologia , Doenças Retinianas/imunologia , Vasculite/etiologia , Vasculite/imunologiaRESUMO
A child with male pseudohermaphroditism, Wilms' tumor, and glomerulonephritis (Drash syndrome) was found to have a proliferative glomerular lesion, extensive renal cortical interstitial and glomerular scarring, and marked renal tubular foam cell change. By electron microscopy, dense deposits were seen in mesangial areas with focal extension into the subendothelial space. Segmental deposits of immunoglobulin and complement were found in the glomeruli by immunofluorescence microscopy. A consistent karyotypic abnormality consisting of isochromosomes of the long arms of 1 and 17, and an interstitial deletion of the long arm of 5 was found in cultures of tumor cells, but no karyotypic abnormalities were found in peripheral blood lymphocytes or non-neoplastic kidney. Renal failure in this case has progressed despite the surgical removal of the tumor. These findings are similar to those reported previously in patients with this syndrome and suggest a common pathophysiological abnormality in all of the patients reported so far.
Assuntos
Transtornos do Desenvolvimento Sexual/complicações , Glomerulonefrite/complicações , Neoplasias Renais/complicações , Tumor de Wilms/complicações , Complexo Antígeno-Anticorpo/análise , Criança , Aberrações Cromossômicas , Proteínas do Sistema Complemento/análise , Glomerulonefrite/patologia , Humanos , Imunoglobulina G/análise , Glomérulos Renais/imunologia , Glomérulos Renais/ultraestrutura , Neoplasias Renais/ultraestrutura , Masculino , Microscopia Eletrônica , Nefrectomia , Diálise Renal , Tumor de Wilms/ultraestruturaRESUMO
The monoclonal antibody, Orthoclone OKT3 (OKT3), has been used with great efficacy in a prospective multicenter trial as therapy for first rejection episodes in cadaveric donor (CD) renal allograft recipients treated with azathioprine (AZA) and prednisone (P). However, although almost all rejection episodes were reversed, recurrent rejection occurred in approximately two-thirds of OKT3-treated patients in this earlier trial; infections also occurred in about two-thirds of patients, often related to the additional immunosuppression necessary to reverse the rerejection episodes. In the current series of patients, OKT3 was used to treat rejection in CD renal graft recipients in a protocol differing from the multicenter trial in two respects: baseline immunosuppression was cyclosporine (CsA) and P or CsA, AZA, and P (probably more potent immunosuppressive combinations than the AZA and P in the multicenter trial); and OKT3 treatment was reserved for rejection episodes resistant to 3 bolus infusions of methylprednisolone (MP), 5-10 mg/kg, rather than as primary therapy for first rejection episodes. Using this protocol, 46 of 74 rejection episodes (62%) diagnosed between 3/85 and 3/86 in CD renal allograft recipients were treated successfully with MP. Of the remaining 28 steroid-resistant rejection episodes, 27 (96%) were reversed with a 7-14-day course of OKT3, 5 mg/day. Only 5 recurrent rejection episodes (19%) have been observed in the 2-14-month follow-up period after OKT3 treatment; infections have occurred in 10 patients (36%), and three grafts (11%) have been lost in OKT3 treated patients. These results suggest that recurrent rejection and subsequent infection after OKT3 is used to treat rejection may be reduced in a protocol where CD renal allograft recipients are treated with baseline immunosuppression regimens including CsA and where OKT3 is reserved for steroid-resistant rejection. This approach appears to be both more cost-effective than, and as effective therapeutically as, treating all first rejection episodes with the monoclonal antibody.
Assuntos
Anticorpos Monoclonais/uso terapêutico , Rejeição de Enxerto , Transplante de Rim , Adolescente , Adulto , Criança , Feminino , Seguimentos , Sobrevivência de Enxerto , Humanos , Terapia de Imunossupressão , Masculino , Pessoa de Meia-Idade , Linfócitos T/classificação , Transplante HomólogoRESUMO
BACKGROUND: Preexisting renal dysfunction has been reported to significantly increase the morbidity and mortality associated with orthotopic liver transplantation (OLT). OLT alone has been recommended for adults and children with end-stage liver disease and reversible causes of renal failure (i.e., hepatorenal syndrome), whereas combined liver and kidney transplantation (LKT) has been shown to be an effective treatment for adults with combined end-stage liver and kidney disease. The purpose of this study was to examine the role of LKT in children. METHODS: Between October of 1984 and 1997, 385 children less than 18 years of age underwent OLT at the University of Chicago. During this same time period 12 patients underwent LKT. Data were gathered by retrospective review of the patients medical records and by interviews conducted with the patients' families. RESULTS: Actuarial patient survival was comparable for children who underwent OLT alone and LKT (69% versus 67% at 5 years). All allograft losses in the LKT group were the result of patient death and occurred within the first 90 postoperative days. Factors associated with decreased patient survival included severity of illness as reflected by United Network of Organ Sharing status and LKT after failed OLT or cadaveric renal transplant. CONCLUSIONS: In children with concomitant endstage liver and kidney disease, LKT can be considered an effective therapeutic option in selected patients. Long-term patient survival in patients undergoing LKT is comparable to that of patients with normal renal function undergoing OLT alone.
Assuntos
Transplante de Rim , Transplante de Fígado , Criança , Pré-Escolar , Rejeição de Enxerto , Humanos , Lactente , Transplante de Rim/mortalidade , Transplante de Fígado/mortalidade , Transplante HomólogoRESUMO
Plasmacytic infiltrates in renal allograft biopsies are uncommon and morphologically distinctive lesions that may represent variants of acute rejection. This study sought significant clinical and pathologic determinants that might have influenced development of these lesions and assessed their prognostic significance. Renal allograft biopsies (n = 19), from 19 patients, with tubulointerstitial inflammatory infiltrates containing abundant plasma cells, composing 32 +/- 8% of the infiltrating mononuclear cells, were classified using Banff '97 criteria. Clonality of the infiltrates was determined by immunoperoxidase staining for kappa and lambda light chains and polymerase chain reaction for immunoglobulin heavy-chain gene rearrangements, using V(H) gene framework 3 and JH consensus primers. In situ hybridization for Epstein-Barr virus encoded RNA (EBER) was performed in 17 cases. The clinical features, histology, and outcome of these cases were compared with kidney allograft biopsies (n = 17) matched for time posttransplantation and type of rejection by Banff '97 criteria, with few plasma cells (7 +/- 5%). Sixteen of 19 biopsies (84%) with plasmacytic infiltrates had EBER-negative (in 14 cases tested) polyclonal plasma cell infiltrates that were classifiable as acute rejection (types 1A [4], 1B [10], and 2A [2]). These biopsies were obtained between 10 and 112 months posttransplantation. Graft loss from acute and/or chronic rejection was 50% at 1 year and 63% at 3 years, and the median time to graft failure was 4.5 months after biopsy. There was no significant difference in overall survival or time to graft failure compared with the controls. Three of 19 biopsies (16%) had EBER-negative polyclonal plasmacytic hyperplasia, mixed monoclonal and polyclonal polymorphous B cell hyperplasia, and monoclonal plasmacytoma-like posttransplantation lymphoproliferative disease (PTLD) and were obtained at 17 months, 12 weeks, and 7 years after transplantation, respectively. Graft nephrectomies were performed at 1, 19, and 5 months after biopsy, respectively. Plasmacytic infiltrates in renal allografts comprise a spectrum of lesions from acute rejection to PTLD, with a generally poor prognosis for long-term graft survival.
Assuntos
Rejeição de Enxerto/patologia , Transplante de Rim/patologia , Transtornos Linfoproliferativos/patologia , Plasmócitos/patologia , Adulto , Biópsia , Contagem de Células , DNA/análise , Infecções por Vírus Epstein-Barr/complicações , Infecções por Vírus Epstein-Barr/patologia , Feminino , Rearranjo Gênico , Genes de Imunoglobulinas , Rejeição de Enxerto/terapia , Rejeição de Enxerto/virologia , Herpesvirus Humano 4/isolamento & purificação , Herpesvirus Humano 4/patogenicidade , Humanos , Técnicas Imunoenzimáticas , Imunossupressores/uso terapêutico , Hibridização In Situ , Transtornos Linfoproliferativos/virologia , Masculino , Pessoa de Meia-Idade , Nefrite Intersticial/patologia , Nefrite Intersticial/virologia , Complicações Pós-Operatórias , RNA Viral/análise , Transplante HomólogoRESUMO
IgA nephropathy has a variable course and leads to renal failure in a substantial number of cases. In an attempt to identify prognostic indicators in this disease, we evaluated the clinical and pathologic findings of 20 unselected patients with IgA nephropathy, 13 of whom were followed for 1.5 to 5 years (mean 2.8 years). Biopsy specimens were obtained from all patients and were examined by light and electron microscopy and by immunofluorescence. The activity and severity of the lesions were graded according to a modified classification used by Meadow et al. for the nephropathy associated with Henoch-Schönlein purpura. The results reveal a correlation between the histopathologic grading in the initial biopsy and the clinical outcome: Patients with mild (grade II) or moderate (grade III) lesions had a benign course or showed evidence of active disease without deterioration of renal function, whereas all patients with grade IV or V lesions who were followed for more than one year developed end-stage renal failure. These observations suggest that histologic grading at initial renal biopsy may be a useful prognostic indicator of the clinical outcome of IgA nephropathy.
Assuntos
Imunoglobulina A/análise , Nefropatias/patologia , Glomérulos Renais/imunologia , Rim/patologia , Adolescente , Adulto , Criança , Feminino , Humanos , Nefropatias/diagnóstico , Nefropatias/imunologia , Testes de Função Renal , Masculino , Pessoa de Meia-Idade , PrognósticoRESUMO
There is increasing evidence that, as in systemic lupus erythematosus (SLE), deposition of immune complexes plays a role in the pathogenesis of dermatitis herpetiformis (DH). Dermatitis herpetiformis and SLE were diagnosed in a 15-year-old girl with Marfan's syndrome who died of cardiac tamponade secondary to cystic medial necrosis of the ascending aorta. The concurrence of these diseases suggests that predisposition to immune-mediated disorders may be associated with the expression of multiple clinical entities.
Assuntos
Dermatite Herpetiforme/complicações , Lúpus Eritematoso Sistêmico/complicações , Síndrome de Marfan/complicações , Adolescente , Complexo Antígeno-Anticorpo , Dermatite Herpetiforme/imunologia , Feminino , HumanosRESUMO
In the general population, an estimated 70% or more of premature morbidity can be attributed to tobacco use, undertreatment of hypertension, and obesity. From a public health perspective, health-related behaviors that reduce the risk for cardiovascular disease should be encouraged for all children and their families. Pediatricians are obligated to accurately and frequently monitor patients' blood pressures. When discovered, elevated blood pressure should be appropriately investigated, with the evaluation being tailored to the age of the child and to the severity of the blood pressure elevation. Investigation should focus on not only a search for a cause but also target organ effects. Timely recognition of abnormal blood pressure and appropriate interventions are necessary to affect the future development of cardiovascular and renal morbidity and mortality.
Assuntos
Hipertensão , Adolescente , Fatores Etários , Anti-Hipertensivos/uso terapêutico , Pressão Sanguínea , Determinação da Pressão Arterial/instrumentação , Determinação da Pressão Arterial/métodos , Estatura , Criança , Pré-Escolar , Feminino , Humanos , Hipertensão/diagnóstico , Hipertensão/etiologia , Hipertensão/terapia , Lactente , Recém-Nascido , Masculino , Pediatria , Atenção Primária à Saúde , Grupos Raciais , Valores de Referência , Caracteres SexuaisRESUMO
A 19-year-old woman with systemic lupus erythematosus had choroidal fluorescein leakage into discreet areas of subretinal fluid. The findings were abolished with corticosteroids. A choroidal vasculitis was suggested by mononuclear infiltration and immunofluorescent demonstration of immunoglobulin.
Assuntos
Coriorretinite/etiologia , Coriorretinite/imunologia , Lúpus Eritematoso Sistêmico/complicações , Corticosteroides/uso terapêutico , Adulto , Coriorretinite/tratamento farmacológico , Túnica Conjuntiva/imunologia , Feminino , Angiofluoresceinografia , Imunofluorescência , Humanos , Imunoglobulina G/análise , Lúpus Eritematoso Sistêmico/imunologia , Úvea/imunologiaAssuntos
Anticorpos Monoclonais/uso terapêutico , Antígenos de Superfície/imunologia , Ciclosporinas/uso terapêutico , Rejeição de Enxerto , Transplante de Rim , Anticorpos Monoclonais/efeitos adversos , Antígenos de Diferenciação de Linfócitos T , Cadáver , Humanos , Terapia de Imunossupressão , Infecções/etiologia , Linfócitos T/imunologiaRESUMO
Twenty cadaveric renal transplant patients (mean age 12.5 +/- 4.8 years) received 14 days (mean 13.0 +/- 2.8 days) of OKT3 (mean dose 0.16 +/- 0.09 mg/kg) along with prednisone 0.5 mg/kg per day, azathioprine 2 mg/kg per day and cyclosporine 5 mg/kg per day which was increased to obtain therapeutic levels before the discontinuation of OKT3. Actuarial patient and graft survival were 100% and 50%, respectively, at both 1 and 5 years. Four children lost their grafts within the first 48 h. One loss was technical in origin, the remaining 3 had pathological evidence of vascular thrombosis. Of the remaining 16 children, 12 (75%) experienced rejection episodes within the first 2 months post transplant (mean 27 +/- 15 days). Successful reversal of early rejection episodes was achieved in 11 of 12 patients. Clinically significant cytomegalovirus infection occurred in 4 patients and resulted in graft loss in 2 patients. Circulating OKT3 levels ranging from 1,000 to 32,000 ng/ml were seen in all patients within the first 48 h. There was a rapid and total depletion of circulating CD3-positive lymphocytes in all patients. Anti-isotypic and anti-idiotypic OKT3 antibodies were assessed by enzyme-linked immunosorbent assay (ELISA), and blocking anti-idiotypic antibodies were detected by immunofluorescence inhibition assay. Positive OKT3 antibody titers were detected in 11 children by ELISA and 10 children by immunofluorescence inhibition assay.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Transplante de Rim , Muromonab-CD3/uso terapêutico , Adolescente , Criança , Infecções por Citomegalovirus/imunologia , Infecções por Citomegalovirus/prevenção & controle , Feminino , Rejeição de Enxerto/imunologia , Rejeição de Enxerto/prevenção & controle , Sobrevivência de Enxerto/imunologia , Humanos , Transplante de Rim/efeitos adversos , Transplante de Rim/métodos , Masculino , Muromonab-CD3/efeitos adversos , Muromonab-CD3/sangue , Estudos RetrospectivosRESUMO
A 3-week-old infant with liver failure underwent an orthotopic liver transplant. Following a prolonged second surgical procedure in which he received massive amounts of blood products, his serum calcium was 31.3. mg/dl (7.8 mmol/l). This patient represents a case of severe hypercalcemia secondary to intraoperative calcium infusions given in an effort to overcome infusion-related citrate toxicity in a neonate with hepatic dysfunction.
Assuntos
Hipercalcemia/etiologia , Transplante de Fígado , Complicações Pós-Operatórias , Anticoagulantes/intoxicação , Cálcio/uso terapêutico , Citratos/intoxicação , Ácido Cítrico , Transfusão de Eritrócitos/efeitos adversos , Humanos , Recém-Nascido , Cuidados Intraoperatórios , Masculino , Transfusão de Plaquetas/efeitos adversos , Reação TransfusionalRESUMO
The class of immunoglobulin (Ig) deposited at the dermal-epidermal junction (DEJ) of the skin in patients with systemic lupus erythematosus (SLE) has been proposed to have prognostic implications. The authors studied disease activity in 51 SLE patients with a positive lupus band. Patients with cutaneous IgM deposits had significantly more severe disease than those with only IgG or with mixed immunoglobulin deposits. While their data suggest an association between IgM deposits, severe disease and a poor prognosis, they urge caution in utilizing Ig deposits as a prognostic indicator.
Assuntos
Imunoglobulinas/análise , Lúpus Eritematoso Sistêmico/imunologia , Pele/imunologia , Adolescente , Adulto , Fatores Etários , Idoso , Biópsia , População Negra , Criança , Proteínas do Sistema Complemento/análise , Feminino , Imunofluorescência , Humanos , Imunoglobulina G/análise , Imunoglobulina M/análise , Rim/imunologia , Lúpus Eritematoso Sistêmico/diagnóstico , Lúpus Eritematoso Sistêmico/tratamento farmacológico , Lúpus Eritematoso Sistêmico/genética , Masculino , Pessoa de Meia-Idade , Prognóstico , População BrancaRESUMO
We report the case of a 5-year-old girl who died two years after onset of the idiopathic nephrotic syndrome, which failed to respond to treatment with corticosteroid and cyclophosphamide. Severe atherosclerotic changes were noted in both coronary arteries. Prolonged hyperlipidemia in patients with long-standing nephrotic syndrome may represent a major risk factor predisposing to premature coronary atherosclerosis in children who are also destined to develop chronic renal failure.
Assuntos
Corticosteroides/uso terapêutico , Doença das Coronárias/etiologia , Síndrome Nefrótica/complicações , Pré-Escolar , Doença das Coronárias/patologia , Ciclofosfamida/uso terapêutico , Feminino , Humanos , Síndrome Nefrótica/tratamento farmacológico , Síndrome Nefrótica/patologiaRESUMO
An idiopathic nephrotic syndrome associated with membranous glomerulopathy antedated the subsequent emergence of systemic lupus erythematosus in two patients (7-year-old and 14-year-old girls). At the onset of INS, there was neither clinical evidence of multisystem disease nor unequivocal serologic evidence of SLE. The only early possible indication of SLE was the presence of microtubular inclusions in glomerular endothelial cells on electron microscopy. In each instance (one year and three years after onset of INS), a second renal biopsy showed transformation of the membranous glomerular lesion to a more florid type with glomerular subendothelial dense deposits. One patient died of overwhelming pulmonary infection while she was receiving prednisone and cyclophosphamide; the other developed progressive renal failure despite steroid treatment. SLE should be considered in patients presenting with apparent idiopathic MG, in whom nephrotic syndrome persists. Intraendothelial cell microtubular inclusions may be an early clue to later emergence of SLE.
Assuntos
Lúpus Eritematoso Sistêmico/complicações , Síndrome Nefrótica/complicações , Adolescente , Criança , Feminino , Humanos , Lúpus Eritematoso Sistêmico/diagnóstico , Síndrome Nefrótica/diagnósticoRESUMO
Unlike primary idiopathic childhood hemolytic uremic syndrome (HUS), certain cases of HUS are clearly secondary. This article describes a child who represents the 11th reported case of secondary HUS following bone marrow transplantation, and notably, the fourth such case without exposure to cyclosporine A. The renal biopsy was diagnostic in this case. Therefore, the pathognomonic features of the biopsy, as well as its prognostic implications are described and clarified. This patient was treated with plasma exchange, and is the second reported surviving case of secondary HUS following bone marrow transplantation.
Assuntos
Transplante de Medula Óssea/efeitos adversos , Síndrome Hemolítico-Urêmica/etiologia , Criança , Feminino , Síndrome Hemolítico-Urêmica/patologia , Síndrome Hemolítico-Urêmica/terapia , Humanos , Rim/patologiaRESUMO
We have previously described (Medicine 56:493, 1977) 12 patients with diffuse lupus glomerulonephritis who had no clinical or laboratory evidence of renal involvement at the time of the initial biopsy. In this article we report the course of 10 of these patients followed for 5-11 yr (mean 83 mo). One patient died in renal failure and two others of related causes (septicemia and subarachnoid hemorrhage). Seven patients (Group I) had a benign course from a renal standpoint, with stable renal function and mild or no urinary abnormalities. Repeat biopsy in four patients in this group revealed near complete resolution of the original lesion in two and considerable improvement in two others, who now have primarily mesangial hypercellularity and a focal lesion, respectively. Renal function deteriorated in three patients (Group II), resulting in loss of congruent to 50% of GFR in two and renal death in the third. Repeat biopsy in one of these patients showed a more severe, albeit focal, glomerulonephritis. Prognosis for renal function appears better in patients with silent nephropathy, but larger numbers are required to substantiate this impression. Until definitive answers become available, we believe it prudent to biopsy SLE patients even in the absence of overt renal involvement and to treat those with diffuse proliferative glomerulonephritis.
Assuntos
Glomerulonefrite/patologia , Lúpus Eritematoso Sistêmico/patologia , Adolescente , Adulto , Creatinina/sangue , Imunofluorescência , Seguimentos , Glomerulonefrite/tratamento farmacológico , Humanos , Rim/fisiopatologia , Glomérulos Renais/patologia , Lúpus Eritematoso Sistêmico/imunologia , Microscopia Eletrônica , Prednisona/uso terapêuticoRESUMO
The relationship of borderline infiltrates to acute rejection by Banff criteria in renal allografts of patients receiving only maintenance immunosuppression is not clear. Renal allograft biopsies with borderline lesions that were not treated with additional anti-rejection therapy were retrospectively studied. Sixty-five such biopsies were identified from 50 patients, and their outcome was determined by serum creatinine and/or histologic findings in subsequent biopsies, up to 40 d after the initial biopsy. In addition to the borderline infiltrates, there was evidence of acute cyclosporine or tacrolimus toxicity (58%), acute tubular necrosis (12%), and urinary obstruction (12%). Forty-day follow-up after 30 (46%) biopsies revealed serum creatinine < 110% of baseline, and repeat biopsies were not indicated. In 17 (26%), the serum creatinine initially decreased, then increased, and follow-up biopsies showed acute rejection in nine. In 18 (28%), the creatinine remained elevated and follow-up biopsies revealed acute rejection in nine. The untreated borderline infiltrates were thus nonprogressive after 47 biopsies (72%) and progressed to histologic acute rejection after 18 (28%). When there was increasing or persistently elevated creatinine after the initial biopsy, 51% of cases (18 of 35) progressed to acute rejection. Infiltrates that progressed to rejection had more frequent glomerulitis (7 of 18 versus 3 of 47, P = 0.003) and Banff acute score indices (i+t+v+g) >2 (16 of 18 versus 29 of 47, P = 0.03). A majority (72%) of borderline infiltrates not given additional anti-rejection therapy did not progress to acute rejection over 40 d of follow-up, suggesting that conservative management of these lesions, at least in the short term, may be more appropriate than routine treatment as acute rejection.
Assuntos
Transplante de Rim , Rim/patologia , Adolescente , Adulto , Biópsia , Criança , Pré-Escolar , Creatinina/sangue , Progressão da Doença , Feminino , Humanos , Imunossupressores/intoxicação , Rim/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias , Período Pós-Operatório , Valor Preditivo dos Testes , Estudos RetrospectivosRESUMO
Although FK 506 has been shown to effectively reverse refractory renal allograft rejection, its ability to reverse accelerated renal allograft rejection as a primary agent has not been specifically addressed. Herein evidence of the ability of FK 506 to reverse accelerated renal allograft rejection is presented. A 16-yr-old highly sensitized (PRA 75%) male underwent a second cadaveric renal transplant procedure. Despite induction immunosuppression with ATGAM, cyclosporine, azathioprine, and corticosteroids, a marked elevation in serum creatinine (1.6-->2.1 ng/dl) and reduction in urine output (4000 ml/d-->1000 ml/d) were observed on the sixth post-transplant day. Renal allograft biopsy performed at that time revealed typical features of accelerated rejection including neutrophil margination in glomerular and interstitial capillaries, and C3, IgG, and fibrin deposition in glomerular and interstitial capillaries (by immunofluorescence). FK 506 therapy was promptly instituted and ATGAM therapy discontinued. Serum creatinine peaked within 3 d of FK 506 therapy (2.5 mg/dl) and subsequently progressively dropped to 1.2 mg/dl. Repeat biopsy on FK 506 treatment day 12 revealed marked histologic improvement. Renal function remains excellent (1.3 mg/dl) 18 months after initiation of FK 506 therapy, and recurrent rejection has not been observed. This experience provides evidence that FK 506 therapy may effectively reverse accelerated renal allograft rejection, and that it provides a means for treating antibody-mediated mechanisms of allograft rejection.