RESUMO
In this paper, we discuss the evolution over the last 15 years in the Curie Institute of the concept, the development of the design and some properties of glycoconjugated photosensitizers with the aim to optimize the tumor targeting in photodynamic therapy. By this research, we have shown that specific interactions between a mannose-lectin and trimannosylglycodendrimeric porphyrins contributed to a larger extent than non-specific ones to the overall interaction of a glycosylated tetraarylporphyrin with a membrane. The studies of in vitro photocytotoxicity showed the relevance of the global geometry of the photosensitizer, the number and position of the linked glycopyranosyl groups on the chromophore and their lipophilicity. The two best compounds appeared to be porphyrins bearing three α-glycosyl groups on para-position of meso-phenyl via a flexible linker. Compound bearing α-manosyl moieties was evaluated successfully in two in vivo xenografted animal models of human retinoblastoma and colorectal cancers. Conversely, the presence on the chromophore of three sugars via a glycodendrimeric moiety induced a potential cluster effect, but decreased the in vitro photoefficiency despite a good affinity for a mannose-lectin.
Assuntos
Dendrímeros/síntese química , Glicoconjugados/química , Neoplasias/patologia , Fármacos Fotossensibilizantes/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Dendrímeros/farmacologia , Glicosilação , Humanos , Estrutura Molecular , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Relação Estrutura-AtividadeRESUMO
This paper describes the conception, synthesis, and characterization of new tetrapyrrolic chromophores bearing glycodendrimeric moieties inducing a potential increase of tumor targeting by a cluster effect. Two families of monoglycodendrimeric photosensitizers bearing three glycosyl units were designed, prepared with an acceptable overall efficiency and characterized by NMR, UV-visible, and fluorescence spectroscopies. The polarity and log P were evaluated by HPLC and the stir-flask method, respectively. The in vitro photoefficiency against two human tumor cell lines was assessed. The presence of the glycodendrimeric group does not appear to increase the tumor in vitro targeting.
Assuntos
Dendrímeros/química , Dendrímeros/síntese química , Lipossomos/química , Fármacos Fotossensibilizantes/síntese química , Fármacos Fotossensibilizantes/farmacologia , Porfirinas/síntese química , Tetrapirróis/química , Tetrapirróis/síntese química , Linhagem Celular Tumoral , Cromatografia Líquida de Alta Pressão , Glicosilação , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Fotobiologia , Fotoquimioterapia , Fármacos Fotossensibilizantes/química , Porfirinas/química , Porfirinas/farmacologia , Espectrometria de FluorescênciaRESUMO
Two glycodendrimeric phenylporphyrins were synthesized and their interaction with phospholipids was studied at the air-water interface and in liposome bilayers; such liposomes bearing glycodendrimeric porphyrin could constitute an efficient carrier for drug targeting in photodynamic therapy.