Perspective on Fe0-PS synergetic effect and reaction mechanism in the thallium(I) contaminated water treatment.

Due to extreme toxicity of the element of thallium (Tl), increasing aqueous Tl pollution incidents have aroused growing concerns. As the prevalent and stable form, i.e., monovalent Tl, the highly efficient removal methodologies of Tl(I) from (waste)water remains limited and challenging. In this study, an advanced oxidation method, the feasibility of using zero valent iron (Fe0) coupled with persulfate (PS) to treat Tl(I)-containing synthetic wastewater was investigated. Its influence parameters, including reaction time, initial Tl concentration, dosages of PS and Fe0, initial and coagulation pH, temperature, coexisting ions and organic matter (NO3-, SO42-, Cl- and HA) were examined. The results revealed that the system can be applied to a wide range of pH and temperature and the reaction equilibrium can be reached in about 30 min. Favorable Tl(I) removal rate (>98%) was observed in the synthetic wastewater with medium and relatively high Tl(I) concentration (≤0.250 mM). The analyses of characterization results including electron spin resonance spectrometer and X-ray photoelectron spectroscopy indicated that ·OH played a vital role in the removal of Tl(I), which was oxidized and removed by co-precipitation. Fe0 can be served as a stable source of Fe2+ to efficiently catalyze PS. The remaining Fe0 can be easily separated because of its magnetism, assuring the promising reusability of the reactant. The study aims to provide references for treatment of real Tl polluted wastewater.

Two Facile Aniline-Based Hypercrosslinked Polymer Adsorbents for Highly Efficient Iodine Capture and Removal.

Effective capture and safe disposal of radioactive iodine (129I or 131I) during nuclear power generation processes have always been a worldwide environmental concern. Low-cost and high-efficiency iodine removal materials are urgently needed. In this study, we synthesized two aniline-based hypercrosslinked polymers (AHCPs), AHCP-1 and AHCP-2, for iodine capture in both aqueous and gaseous phases. They are obtained by aniline polymerization through Friedel-Crafts alkylation and Scholl coupling reaction, respectively, with high chemical and thermal stability. Notably, AHCP-1 exhibits record-high static iodine adsorption (250 wt%) in aqueous solution. In the iodine vapor adsorption, AHCP-2 presents an excellent total iodine capture (596 wt%), surpassing the most reported amorphous polymer adsorbents. The rich primary amine groups of AHCPs promote the rapid physical capture of iodine from iodine water and iodine vapor. Intrinsic features such as low-cost preparation, good recyclability, as well as excellent performance in iodine capture indicate that the AHCPs can be used as potential candidates for the removal of iodine from radioactive wastewater and gas mixtures.

Risk mitigation by waste-based permeable reactive barriers for groundwater pollution control at e-waste recycling sites.

Permeable reactive barriers (PRBs) have proved to be a promising passive treatment to control groundwater contamination and associated human health risks. This study explored the potential use of low-cost adsorbents as PRBs media and assessed their longevity and risk mitigation against leaching of acidic rainfall through an e-waste recycling site, of which Cu, Zn, and Pb were the major contaminants. Batch adsorption experiments suggested a higher adsorption capacity of inorganic industrial by-products [acid mine drainage sludge (AMDS) and coal fly ash (CFA)] and carbonaceous recycled products [food waste compost (FWC) and wood-derived biochar] compared to natural inorganic minerals (limestone and apatite). Continuous leaching tests of sand columns with 10 wt% low-cost adsorbents were then conducted to mimic the field situation of acidic rainfall infiltration through e-waste-contaminated soils (collected from Qingyuan, China) by using synthetic precipitation leaching procedure (SPLP) solution. In general, Zn leached out first, followed by Cu, and finally delayed breakthrough of Pb. In the worst-case scenario (e.g., at initial concentrations equal to 50-fold of average SPLP result), the columns with limestone, apatite, AMDS, or biochar were effective for a relatively short period of about 20-40 pore volumes of leaching, after which Cu breakthrough caused non-cancer risk concern and later-stage Pb leaching considerably increased both non-cancer and lifetime cancer risk associated with portable use of contaminated water. In contrast, the columns with CFA or FWC successfully mitigated overall risks to an acceptable level for a prolonged period of 100-200 pore volumes. Therefore, with proper selection of low-cost adsorbents (or their mixture), waste-based PRBs is a technically feasible and economically viable solution to mitigate human health risk due to contaminated groundwater at e-waste recycling sites.

Insights into the effect of hydrochar-derived dissolved organic matter on the sorption of diethyl phthalate onto soil: A pilot mechanism study.

Biowaste-derived hydrochar is an emerging close-to-natural product and has shown promise for soil improvement and remediation, but the environmental behavior of the dissolved organic matter released from hydrochar (HDOM) is poorly understood. Focusing on the typical mulch film plasticizer diethyl phthalate (DEP), we investigated the effect of HDOM on the sorption behavior of DEP on soil. The relatively low concentration of HDOM (10 mg L-1, 25 mg L-1) decreases the sorption quantity of DEP on soil, while it increases by a relatively high concentration, 50 mg L-1. The transformation from multilayer to monolayer sorption of DEP on soil occurs as the concentration of HDOM increases. The tryptophan-like substance is the main component of HDOM sorbed to soil, reaching 49.82 %, and results in competition sorption with DEP. The soil pores are blocked by HDOM, which limits the pore filling and mass transfer of DEP, but partitioning is significantly enhanced. The surface functional groups in HDOM are similar to those in soil, and chemical sorption, mainly composed of hydrogen bonding, exists but is not significantly strengthened. We identified the specific impact of HDOM on the sorption of organic pollutants on soil and provide new insights into the understanding of the environmental behavior of hydrochar.

Inoculation with Bacillus thuringiensis reduces uptake and translocation of Pb/Cd in soil-wheat system: A life cycle study.

Microbial inoculation is an important strategy to reduce the supply of heavy metals (HMs) in soil-crop systems. However, the mechanisms of microbial inoculation for the availability of HMs in soil and their accumulation/transfer in crops remain unclear. Here, the inhibitory effect of inoculation with Bacillus thuringiensis on the migration and accumulation of Pb/Cd in the soil-wheat system during the whole growth period was investigated by pot experiments. The results showed that inoculation with Bacillus thuringiensis increased soil pH and available nutrients (including carbon, nitrogen, and phosphorus), and enhanced the activities of nutrient-acquiring enzymes. Dominance analysis showed that dissolved organic matter (DOM) is the key factor affecting the availability of HMs. The content of colored spectral clusters and humification characteristics of DOM were significantly improved by inoculation, which is conducive to reducing the availability of Pb/Cd, especially during the flowering stage, the decrease was 12.8 %. Inoculation decreased Pb/Cd accumulation in the shoot and the transfer from root to shoot, with the greatest decreases at the jointing and seedling stages (27.0-34.1 % and 6.9-11.8 %), respectively. At the maturity stage, inoculation reduced the Pb/Cd accumulation in grain (12.9-14.7 %) and human health risk (4.1-13.2 %). The results of Pearson correlation analysis showed that the availability of Pb/Cd was positively correlated with the humification of DOM. Least square path model analysis showed that Bacillus thuringiensis could significantly reduce Pb/Cd accumulation in the grain and human health risks by regulating DOM spectral characteristics, the availability of HMs in soil and metals accumulation/transport in wheat at different growth stages. This study revealed the inhibition mechanism of Bacillus thuringiensis on migration of Pb/Cd in a soil-wheat system from a viewpoint of a full life cycle, which offers a valuable reference for the in-situ remediation of HM-contaminated soil and the safe production of food crops in field.

Pb isotopic fingerprinting of uranium pollution: New insight on uranium transport in stream-river sediments.

Uranium mill tailings (UMT) present a significant environmental concern due to high levels of radioactive and toxic elements, including uranium (U), thorium (Th), and lead (Pb), which can pose serious health risks to aquatic ecosystems. While Pb isotopic tracers have been widely utilized in environmental studies to identify elemental sources and geological processes, their application in U geochemistry remains relatively limited. In this study, we investigate the distribution and migration of U in stream-river sediments surrounding a decommissioned U hydrometallurgical area, employing Pb isotopes as tracers. Our findings reveal significant enrichment and ecological risk of U, Pb, and Th in the sediments. Uranium predominantly associates with quartz and silicate minerals, and its dispersion process is influenced by continuous leaching and precipitation cycles of typical U-bearing minerals. Furthermore, we establish a compelling positive relationship (r2 = 0.97) between 208Pb/207Pb and 206Pb/207Pb in the stream-river sediments and sediment derived from UMT. Application of a binary Pb mixing model indicates that anthropogenic hydrometallurgical activities contribute to 2.5-62.7% of the stream-river sediments. Notably, these values are lower than the 6.6-89.6% recorded about 10 years ago, prior to the decommissioning of the U hydrometallurgical activity. Our results underscore the continued risk of U pollution dispersion even after decommission, highlighting the long-term environmental impact of UMT.

Synergistic enhancement of plant growth and cadmium stress defense by Azospirillum brasilense and plant heme: Modulating the growth-defense relationship.

Plant growth-promoting rhizobacteria (PGPR) play important roles in plant growth and defense under heavy metal (HM) stress. The direct integration of microbial and plant signals is key to the regulation of plant growth and HM stress defense, but the underlying mechanisms are still limited. Herein, we reveal a novel mechanism by which PGPR regulates plant growth-regulating substances in plant tissues and coordinates plant growth and defense in pak choi under cadmium (Cd) stress. This might be an efficient strategy and an extension of the mechanism by which plant-microbe interactions improve plant stress resistance. Azospirillum brasilense and heme synergistically reduced the shoot Cd content and promoted the growth of pak choi. The interaction between abscisic acid of microbial origin and heme improved Cd stress tolerance through enhancing Cd accumulation in the root cell wall. The interaction between A. brasilense and heme induced the growth-defense shift in plants under Cd stress. Plants sacrifice growth to enhance Cd stress defense, which then transforms into a dual promotion of both growth and defense. This study deepens our understanding of plant-microbe interactions and provides a novel strategy to improve plant growth and defense under HM stress, ensuring future food production and security.

Arsenic adsorption by different Fe-enriched biochars conditioned with sulfuric acid.

In this study, ferric chloride and sulfuric acid were used to increase the Fe-containing minerals on the biochar surface before a pyrolysis at 600 °C. The pristine and Fe-modified biochars prepared at different concentrations of sulfuric acid (50FBC and 72FBC) were characterized and analyzed, and their capacity of As(V) adsorption under various pH and ionic strength were evaluated. The results showed that the maximum adsorption capacities of As(V) calculated by the Langmuir model for 50FBC and 72FBC are 10.33 and 15.61 mg g-1, respectively, which are enhanced by 5.0 and 7.8 times compared with the pristine biochar. The higher dosage of H2SO4 (72%) used in the modification leads to a better adsorption capacity of As, especially under neutral to alkaline conditions (7.0 < pH < 10.0). It might result from the increased amounts of Fe-containing minerals formed on the biochar surface, and the enriched functional groups such as phenolic hydroxyl and carboxyl, resulting in the resistance to alkaline conditions. Overall, the Fe-modified biochar, especially 72FBC, had good potential as an environmentally friendly adsorbent for removing As from contaminated water under a wider pH range.

Environmental and health risk assessment of potentially toxic trace elements in soils near uranium (U) mines: A global meta-analysis.

Soil pollution by potentially toxic trace elements (PTEs) near uranium (U) mines arouses a growing interest worldwide. However, nearly all studies have focused on a single site or only a few sites, which may not fully represent the soil pollution status at the global scale. In this study, data of U, Cd, Cr, Pb, Cu, Zn, As, Mn, and Ni contents in U mine-associated soils were collected and screened from published articles (2006-2021). Assessments of pollution levels, distributions, ecological, and human health risks of the nine PTEs were analysed. The results revealed that the average contents of the U, Cd, Cr, Pb, Cu, Zn, As, Mn, and Ni were 39.88-, 55.33-, 0.88-, 3.81-, 3.12-, 3.07-, 9.26-, 1.83-, and 1.17-fold greater than those in the upper continental crust, respectively. The pollution assessment showed that most of the studied soils were heavily polluted by U and Cd. Among them, the U mine-associated soils in France, Portugal, and Bulgaria exhibited significantly higher pollution levels of U and Cd when compared to other regions. The average potential ecological risk value for all PTEs was 3358.83, which indicated the presence of remarkably high risks. Among the PTEs, Cd and U contributed more to the potential ecological risk than the other elements. The health risk assessment showed that oral ingestion was the main exposure route for soil PTEs; and the hazard index (HI) values for children were higher than those for adult males and females. For adult males and females, all hazard index values for the noncarcinogenic risks were below the safe level of 1.00. For children, none of the HI values exceeded the safe level, with the exception of U (HI = 3.56) and As (HI = 1.83), but Cu presented unacceptable carcinogenic risks. This study provides a comprehensive analysis that demonstrates the urgent necessity for treating PTE pollution in U mine-associated soils worldwide.

Microbial metabolic limitation of rhizosphere under heavy metal stress: Evidence from soil ecoenzymatic stoichiometry.

Slow nutrient turnover and destructed soil function were the main factors causing low efficiency in phytoremediation of heavy metal (HM)-contaminated soil. Soil ecoenzymatic stoichiometry can reflect the ability of soil microorganisms to acquire energy and nutrients, and drive nutrient cycling and carbon (C) decomposition in HM-contaminated soil. Therefore, for the first time, we used the enzymatic stoichiometry modeling to examine the microbial nutrient limitation in rhizospheric and bulk soil of different plants (Medicago sativa, Halogeton arachnoideus and Agropyron cristatum) near the Baiyin Copper Mine. Results showed that the main pollutants in this area were Cu, Zn, Cd, and Pb, while Cd and Zn have the greatest contribution according to the analysis of pollution load index (PLI). The activities of soil C-, nitrogen (N)-, and phosphorus (P)-acquiring enzymes in the rhizosphere of plants were significantly greater than that in bulk soil. Moreover, microbial C and P limitations were observed in all plant treatments, while the lower limitation was generally in the rhizosphere compared to bulk soil. The HM stress significantly increased microbial C limitation and decreased microbial P limitation, especially in the rhizospheric soil. The partial least squares path modeling (PLS-PM) further indicated that HM concentration has the greatest effects on microbial P limitation (-0.64). In addition, the highest enzyme activities and the lowest P limitation were observed in the rhizospheric and bulk soil of M. sativa, thereby implying that soil microbial communities under the remediation of M. sativa were steadier and more efficient in terms of their metabolism. These findings are important for the elucidation of the nutrient cycling and microbial metabolism of rhizosphere under phytoremediation, and provide guidance for the restoration of HM-contaminated soil.

Phytoremediation of potentially toxic elements (PTEs) contaminated soils using alfalfa (Medicago sativa L.): A comprehensive review.

Soil contamination with potentially toxic elements (PTEs) is an increasing environmental problem, posing serious threats to the living organisms. Phytoremediation is a sustainable and highly accepted technology for remediation of PTE-contaminated soils. Alfalfa has been widely adopted for the phytoremediation of PTE-contaminated soils due to its large biomass productivity, high PTE tolerance, and strong capacity to take up PTEs. However, there are still no literature reviews systematically summarized the potential of alfalfa in the phytoremediation. Therefore, we review the available literatures that present its PTE uptake, phytotoxicity, tolerance mechanisms, and aided techniques improving the phytoremediation efficiency. In this review, alfalfa shows high amounts of PTEs accumulation, especially in their root tissue. Meanwhile, the inner mechanisms of PTE tolerance and accumulation in alfalfa are discussed including: (i) the activation of antioxidant enzyme system, (ii) subcellular localization, (iii) production of glutathione, phytochelatins, and proline, and (iv) regulation of gene expression. Indeed, excessive PTE can overcome the defense system, which causes oxidative damage in alfalfa plants, thereby inhibiting growth and physiological processes and weakening the ability of PTE uptake. Till now, several approaches have been developed to improve the tolerance and/or accumulation of PTE in alfalfa plants as follows: (i) selection of PTE tolerant cultivars, (ii) applying plant growth regulators, (iii) addition of chelating agents, fertilizer, and biochar materials, and (iv) inoculation of soil microbes. Finally, we indicate that the selection of PTE-tolerant cultivars along with inoculation of soil microbes may be an efficient and eco-friendly strategy of the soil PTE phytoremediation.

Thallium and potentially toxic elements distribution in pine needles, tree rings and soils around a pyrite mine and indication for environmental pollution.

In this study, the distributions of thallium (Tl), and other potential toxic elements, such as Cd, Co, Cu, Pb, Sr, As, Cr, Ni, Zn, and Mn in needles, tree rings and soils of pine trees in one of the largest pyrite mining areas in the world, i.e., Yunfu, China were investigated. The results showed that pseudo-total Tl concentration of the tree rings ranged from 0.41 to 2.03 mg/kg (average: 1.12 mg/kg) during the year of 1998 to 2011. This indicates an overall obvious enrichment of Tl. Further investigation of element level variations in the pine needles showed a negative correlation between Tl content and the distance from the mining area. The results of Principal Component Analysis additionally demonstrated that Tl in the tree rings was most likely derived from the pine needles. Notably, Tl contents in the tree rings exhibited generally similar distribution pattern to the annual production intensity of Yunfu pyrite mining activities. The findings suggest that metal(loid)s in particular of Tl in pine tree rings can be used as alternative proxies to approximatively reconstruct the chronological change of atmospheric environmental pollution induced by pyrite associated mining/smelting activities.

Uptake, organ distribution and health risk assessment of potentially toxic elements in crops in abandoned indigenous smelting region.

Inorganic pollution induced by smelting waste has threatened the safety of environment, whereas the impacts on farmlands with regards to potentially toxic elements (PTEs) receive insufficient attention. Herein, the contents, transfer pathways and potential risks of the PTEs in common crops were examined from different farmlands distributed around an indigenous Zn-smelting area in Guizhou, China. The results showed that Tl in cabbage (Brassica oleracea L.) (up to 3.74 mg/kg) and radish (Raphanus sativus L.) (up to 1.16 mg/kg) at some sites exceeded the maximum permissible level (MPL) (0.5 mg/kg) for food, and, under the same pollution condition, cabbage and radish were more likely to enrich PTEs, and the edible portion of maize was not prone to Tl risk. Hazard quotient calculations of Tl, Ba, and U were greater than 1, indicating the edible risk of crops for these PTEs. Further characterization of selected soils revealed that MnFe2O4 and Fe2O3 controlled the phase transformation of Tl(III) in rhizospheric soils. Furthermore, distinctive mullite was detected in the soil which confirmed the contribution of high temperature smelting to PTEs pollution. The findings indicate an emergent need for soil remediation around historical indigenous metal smelting areas for the sake of food security.

Phosphate Removal Mechanisms in Aqueous Solutions by Three Different Fe-Modified Biochars.

Iron-modified biochar can be used as an environmentally friendly adsorbent to remove the phosphate in wastewater because of its low cost. In this study, Fe-containing materials, such as zero-valent iron (ZVI), goethite, and magnetite, were successfully loaded on biochar. The phosphate adsorption mechanisms of the three Fe-modified biochars were studied and compared. Different characterization methods, including scanning electron microscopy/energy-dispersive spectrometry (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS), were used to study the physicochemical properties of the biochars. The dosage, adsorption time, pH, ionic strength, solution concentration of phosphate, and regeneration evaluations were carried out. Among the three Fe-modified biochars, biochar modified by goethite (GBC) is more suitable for phosphate removal in acidic conditions, especially when the pH = 2, while biochar modified by ZVI (ZBC) exhibits the fastest adsorption rate. The maximum phosphate adsorption capacities, calculated by the Langmuir-Freundlich isothermal model, are 19.66 mg g-1, 12.33 mg g-1, and 2.88 mg g-1 for ZBC, GBC, and CSBC (biochar modified by magnetite), respectively. However, ZBC has a poor capacity for reuse. The dominant mechanism for ZBC is surface precipitation, while for GBC and CSBC, the major mechanisms are ligand exchange and electrostatic attraction. The results of our study can enhance the understanding of phosphate removal mechanisms by Fe-modified biochar and can contribute to the application of Fe-modified biochar for phosphate removal in water.

U(VI) adsorption by green and facilely modified Ficus microcarpa aerial roots: Behavior and mechanism investigation.

Uranium (U)-containing wastewater poses serious pressure to human health and environmental safety. The treatment of U-bearing wastewater using green and facilely fabricated materials is considered a promising alternative. Herein, the raw and modified aerial roots of Ficus microcarpa (RARF and MARF, respectively) were prepared and applied to the treatment of synthesized U-containing wastewater. The results showed that the adsorption process was spontaneous and chemically controlled, which was in good accordance with the pseudo-second-order kinetic and the Redlich-Peterson isotherm adsorption model. The adsorption mechanisms were proposed to be the complexation between U(VI) and oxygen/phosphorus-containing functional groups on MARF.

Nitrogen of EDDS enhanced removal of potentially toxic elements and attenuated their oxidative stress in a phytoextraction process.

(S,S)-ethylenediaminedisuccinic acid (EDDS) has a strong capacity to mobilize potentially toxic elements (PTEs) in phytoextraction. It can release NH4+-N via biodegradation, which can enhance N supply to soil thereafter promote plant growth and plant resistance to PTEs. However, the advanced feature of released N in the EDDS-enhanced phytoextraction remains unclear. In the current study, the effects of N supply released from EDDS on ryegrass phytoextraction and plant resistance to PTEs were investigated in detail by a comparison with urea. Our results supported that the addition of both EDDS and urea increased N concentration in soil solution, yet EDDS needed more time to release available N for plant uptake and transported more N from root to shoot. Additionally, EDDS significantly increased the concentration of all targeted PTEs, i.e. Cu, Zn, Cd, and Pb, in the soil solution, which results in higher levels of their occurrence in plant biomass compared with urea. By contrast, the supply of N slightly enhanced the ryegrass uptake of micro-nutrients, i.e. Cu and Zn, yet it caused negligible effects on nonessential elements, i.e. Cd and Pb. The mobilized PTEs by EDDS lead to elevated oxidative stress because higher levels of malondialdehyde and O2•- were observed. The supply of N attenuated oxidative stress caused by O2•- and H2O2, which was associated with enhanced activities of superoxide dismutase and peroxidase. Our results advanced the understanding of the exogenous N supply and metal resistance mechanisms in the EDDS-enhanced phytoextraction. This study also highlighted that EDDS can serve as a N source to ease N-deficient problems in PTEs-contaminated soils.

Cadmium isotopic fractionation in lead-zinc smelting process and signatures in fluvial sediments.

Cadmium (Cd) is a toxic heavy metal pollutant. Various industrial activities, especially metal smelting, are the main sources of Cd pollution. Cd isotopes have exhibited the ability to be excellent source tracers and can be used to assess the pollution contributions from different sources. Herein, in a typical lead-zinc smelter, Shaoguan, China, significant Cd isotopic fractionation was found during the high temperature smelting process and followed a Rayleigh distillation model. The heavier Cd isotopes were concentrated in the slag, while the lighter Cd isotopes were concentrated in the dust. In the downstream sediment profile of the smelter, sediments have extremely high Cd concentrations that far exceed the Chinese background sediment, indicating severe pollution levels. The ε114/110Cd of the sediment core, ranged from - 0.62 ± 0.5-1.73 ± 0.5, are found between slag (ε114/110Cd=10.42) and dust (ε114/110Cd=-5.68). The binary mixture model suggests that 88-93% of the Cd in sediment profile was derived from the slag, and 7-12% from the deposition of dust. The findings demonstrate the great potential to apply Cd isotopes as a new geochemical tool to distinguish anthropogenic sources and quantify the contribution from various sources in the environment.

Improvement of alfalfa resistance against Cd stress through rhizobia and arbuscular mycorrhiza fungi co-inoculation in Cd-contaminated soil.

Rhizobia and arbuscular mycorrhiza fungi (AMF) are important symbiotic microbes that are advantageous to plants growing in metal-contaminated soil. However, it remains unclear how inoculated microbes affect rhizosphere microbial communities or whether subsequent changes in rhizosphere microbiomes contribute to improving plant resistance under metal stress. This study investigated the effects of rhizobia and AMF inoculation on alfalfa resistance to Cd stress. The response of rhizosphere microbial communities to inoculation and its role in increasing alfalfa' ability to cope with stress were further analyzed using high-throughput sequencing of 16S and ITS rRNA genes. Results showed that single rhizobia or AMF inoculation significantly improved alfalfa resistance to Cd stress, while their co-inoculation resulted in the greatest overall improvement. Improved resistance was reflected by the significant mitigation of Cd-induced lipid peroxidation and reactive oxygen species (ROS) stress caused by increases in antioxidant enzyme activities along with co-inoculation. Furthermore, co-inoculation significantly altered the rhizosphere microbial community structure by decreasing fungal community diversity and increasing bacterial community diversity. Results of partial least squares path modeling (PLS-PM) and variation partitioning analysis (VPA) showed that the rhizosphere bacterial community predominated over the fungal community with respected to improvements in resistance to Cd stress under the co-inoculation treatments. This improvement was specifically seen in the enrichment of certain key bacterial taxa (including Proteobacteria, Actinobacteria, Acidobacteria, and Chloroflexi) induced by the rhizobia and AMF co-inoculation, enhancing alfalfa' ability to uptake rhizosphere nutrients and reduce its release of photosynthetically-derived carbon (C) into soil. Our findings revealed that the co-inoculation of multiple symbiotic microbes can assist plants to effectively cope with Cd stress, providing a greater understanding of rhizosphere bacterial taxa in the microbe-induced phytomanagement.

Lignin valorization by bacterial genus Pseudomonas: State-of-the-art review and prospects.

The most prominent aromatic feedstock on Earth is lignin, however, lignin valorization is still an underrated subject. The principal preparatory strategies for lignin valorization are fragmentation and depolymerization which help in the production of fuels and chemicals. Owing to lignin's structural heterogeneity, these strategies result in product generation which requires tedious separation and purification to extract target products. The bacterial genus Pseudomonas has been dominant for its lignin valorization potency, owing to a robust enzymatic machinery that is used to funnel variable lignin derivatives into certain target products such as polyhydroxyalkanotes (PHAs) and cis, cis-muconic acid (MA). In this review, the potential of genus Pseudomonas in lignin valorization is critically reviewed along with the advanced genetic techniques and tools to ease the use of lignin/lignin-model compounds for the synthesis of bioproducts. This review also highlights the research gaps in lignin biovalorization and discuss the challenges and possibilities for future research.

Particulate plastics-plant interaction in soil and its implications: A review.

Particulate plastics (<5 mm), including macroplastics (1 µm to 5 mm), microplastics (100 nm to 1 µm) and nanoplastics (<100 nm), have become a global environmental problem due to their widespread occurrence, distribution and ecosystem risk. Although numerous studies on particulate plastics have been conducted in aquatic systems, investigations in the soil ecosystem are lacking. Soil is the main storage place of particulate plastics, conferring significant impacts on plant growth and development. The impact of particulate plastics on plants is directly related to the safety of agricultural products. This review comprehensively examines the pollution characteristics and exposure pathways of particulate plastics in agricultural soils, highlighting plastic uptake process, and mechanisms in plants, and effects of particulate plastics, biodegradable particulate plastics and combined pollution of plastics with other environmental pollutants on plant performances. This review identifies a number of future research prospects including the development of accurate quantitative methods for plastic analysis in soil and plant samples, understanding the environmental behaviors of conventional and biodegradable particulate plastics in the presence and absence of other environmental pollutants, unravelling the fate of particulate plastics in plants, phyto-toxicity and molecular regulatory mechanisms of particultate plastics, and developing best management practices for the production of safe agricultural products in plastic-contaminated soils.