RESUMO
Human cerebral cortical malformations are associated with progenitor proliferation and neuronal migration abnormalities. Progenitor cells include apical radial glia, intermediate progenitors and basal (or outer) radial glia (bRGs or oRGs). bRGs are few in number in lissencephalic species (e.g. the mouse) but abundant in gyrencephalic brains. The LIS1 gene coding for a dynein regulator, is mutated in human lissencephaly, associated also in some cases with microcephaly. LIS1 was shown to be important during cell division and neuronal migration. Here, we generated bRG-like cells in the mouse embryonic brain, investigating the role of Lis1 in their formation. This was achieved by in utero electroporation of a hominoid-specific gene TBC1D3 (coding for a RAB-GAP protein) at mouse embryonic day (E) 14.5. We first confirmed that TBC1D3 expression in wild-type (WT) brain generates numerous Pax6+ bRG-like cells that are basally localized. Second, using the same approach, we assessed the formation of these cells in heterozygote Lis1 mutant brains. Our novel results show that Lis1 depletion in the forebrain from E9.5 prevented subsequent TBC1D3-induced bRG-like cell amplification. Indeed, we observe perturbation of the ventricular zone (VZ) in the mutant. Lis1 depletion altered adhesion proteins and mitotic spindle orientations at the ventricular surface and increased the proportion of abventricular mitoses. Progenitor outcome could not be further altered by TBC1D3. We conclude that disruption of Lis1/LIS1 dosage is likely to be detrimental for appropriate progenitor number and position, contributing to lissencephaly pathogenesis.
Assuntos
1-Alquil-2-acetilglicerofosfocolina Esterase/genética , Lisencefalia , Proteínas Associadas aos Microtúbulos/genética , Malformações do Sistema Nervoso , Animais , Dineínas/genética , Células Ependimogliais/metabolismo , Proteínas Ativadoras de GTPase/genética , Lisencefalia/genética , Camundongos , Mitose , Mutação , Malformações do Sistema Nervoso/genéticaRESUMO
Radial glial (RG) cells are the neural stem cells of the developing neocortex. Apical RG (aRG) cells can delaminate to generate basal RG (bRG) cells, a cell type associated with human brain expansion. Here, we report that aRG delamination is regulated by the post-Golgi secretory pathway. Using in situ subcellular live imaging, we show that post-Golgi transport of RAB6+ vesicles occurs toward the minus ends of microtubules and depends on dynein. We demonstrate that the apical determinant Crumbs3 (CRB3) is also transported by dynein. Double knockout of RAB6A/A' and RAB6B impairs apical localization of CRB3 and induces a retraction of aRG cell apical process, leading to delamination and ectopic division. These defects are phenocopied by knockout of the dynein activator LIS1. Overall, our results identify a RAB6-dynein-LIS1 complex for Golgi to apical surface transport in aRG cells, and highlights the role of this pathway in the maintenance of neuroepithelial integrity.
Assuntos
Dineínas , Proteínas rab de Ligação ao GTP , Dineínas/genética , Dineínas/metabolismo , Complexo de Golgi/metabolismo , Humanos , Proteínas Associadas aos Microtúbulos/genética , Proteínas Associadas aos Microtúbulos/metabolismo , Microtúbulos/metabolismo , Neurônios/metabolismo , Proteínas rab de Ligação ao GTP/genética , Proteínas rab de Ligação ao GTP/metabolismoRESUMO
Genetic findings reported by our group and others showed that de novo missense variants in the KIF2A gene underlie malformations of brain development called pachygyria and microcephaly. Though KIF2A is known as member of the Kinesin-13 family involved in the regulation of microtubule end dynamics through its ATP dependent MT-depolymerase activity, how KIF2A variants lead to brain malformations is still largely unknown. Using cellular and in utero electroporation approaches, we show here that KIF2A disease-causing variants disrupts projection neuron positioning and interneuron migration, as well as progenitors proliferation. Interestingly, further dissection of this latter process revealed that ciliogenesis regulation is also altered during progenitors cell cycle. Altogether, our data suggest that deregulation of the coupling between ciliogenesis and cell cycle might contribute to the pathogenesis of KIF2A-related brain malformations. They also raise the issue whether ciliogenesis defects are a hallmark of other brain malformations, such as those related to tubulins and MT-motor proteins variants.
Assuntos
Cílios/genética , Cinesinas/metabolismo , Malformações do Desenvolvimento Cortical/genética , Proteínas Repressoras/metabolismo , Animais , Encéfalo/metabolismo , Ciclo Celular/genética , Cílios/fisiologia , Células HeLa , Humanos , Cinesinas/genética , Malformações do Desenvolvimento Cortical/metabolismo , Camundongos , Microcefalia/metabolismo , Microtúbulos/metabolismo , Neurogênese , Proteínas Repressoras/genética , Fuso Acromático/metabolismo , Tubulina (Proteína)/metabolismoRESUMO
Over the last years, the critical role of cytoskeletal proteins in cortical development including neuronal migration as well as in neuronal morphology has been well established. Inputs from genetic studies were provided through the identification of several mutated genes encoding either proteins associated with microtubules (DCX, LIS1, KIF2A, KIF5C, DYNC1H1) or tubulin subunits (TUBA1A, TUBB2B, TUBB5 and TUBG1), in malformations of cortical development (MCD). We also reported the identification of missense mutations in TUBB3, the postmitotic neuronal specific tubulin, in six different families presenting either polymicrogyria or gyral disorganization in combination with cerebellar and basal ganglial abnormalities. Here, we investigate further the association between TUBB3 mutations and MCDs by analyzing the consequences of Tubb3 knockdown on cortical development in mice. Using the in utero-electroporation approach, we demonstrate that Tubb3 knockdown leads to delayed bipolar morphology and radial migration with evidence, suggesting that the neuronal arrest is a transient phenomenon overcome after birth. Silenced blocked cells display a round-shape and decreased number of processes and a delay in the acquisition of the bipolar morphology. Also, more Tbr2 positive cells are observed, although less cells express the proliferation marker Ki67, suggesting that Tubb3 inactivation might have an indirect effect on intermediate progenitor proliferation. Furthermore, we show by rescue experiments the non-interchangeability of other beta-tubulins which are unable to rescue the phenotype. Our study highlights the critical and specific role of Tubb3 on the stereotyped morphological changes and polarization processes that are required for initiating radial migration to the cortical plate.
Assuntos
Movimento Celular , Córtex Cerebral/metabolismo , Malformações do Desenvolvimento Cortical/genética , Tubulina (Proteína)/metabolismo , Animais , Proteína Duplacortina , Eletroporação , Feminino , Técnicas de Silenciamento de Genes , Humanos , Malformações do Desenvolvimento Cortical/patologia , Camundongos , Mutação de Sentido Incorreto , Gravidez , Isoformas de Proteínas , Tubulina (Proteína)/genéticaRESUMO
In rodents and most other mammals studied, neuronal precursors generated in the subventricular zone (SVZ) migrate to the adult olfactory bulb (OB) to differentiate into interneurons called granule and periglomerular cells. How the newborn cells navigate in the postnatal forebrain to reach precisely their target area is largely unknown. However, it is often thought that postnatal neurogenesis recapitulates the neuronal development occurring during embryogenesis. During brain development, intracellular kinases are key elements for controlling cell polarization as well as the coupling between polarization and cellular movement. We show here that the polarity kinase MARK2 maintains its expression in the postnatal SVZ-OB system. We therefore investigated the potential role of this kinase in adjusting postnatal neuroblast migration. We employed mouse brain slices maintained in culture, in combination with lentiviral vector injections designed to label neuronal precursors with GFP and to diminish the expression of MARK2. Time-lapse video microscopy was used to monitor neuroblast migration in the postnatal forebrain from SVZ precursors to cells populating the OB. We found that reduced MARK2 expression resulted in altered migratory patterns and stalled neuroblasts in the rostral migratory stream (RMS). In agreement with the observed migratory defects, we report a diminution of the proportion of cells reaching the OB layers. Our study reveals the involvement of MARK2 in the maintenance of the migratory direction in postnatally-generated neuroblasts and consequently on the control of the number of newly-generated neurons reaching and integrating the appropriate target circuits.
Assuntos
Proteínas de Ciclo Celular/metabolismo , Movimento Celular/fisiologia , Células-Tronco Neurais/enzimologia , Neurogênese/fisiologia , Bulbo Olfatório/citologia , Proteínas Serina-Treonina Quinases/metabolismo , Animais , Animais Recém-Nascidos , Proteínas de Ciclo Celular/fisiologia , Movimento Celular/genética , Proteínas de Fluorescência Verde/genética , Interneurônios/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Células-Tronco Neurais/citologia , Células-Tronco Neurais/fisiologia , Neurogênese/genética , Bulbo Olfatório/crescimento & desenvolvimento , Bulbo Olfatório/fisiologia , Técnicas de Cultura de Órgãos , Proteínas Serina-Treonina Quinases/fisiologiaRESUMO
Neuronal precursors are continuously integrated into the adult olfactory bulb (OB). The vast majority of these precursor cells originates from the subventricular zone and migrates along the rostral migratory stream (RMS) en route to the OB. This process, called postnatal neurogenesis, results from intricate pathways depending both on cell-autonomous factors and extrinsic regulation provided by the local environment. Using electroporation in postnatal mice to label neuronal precursors with green fluorescent protein (GFP) and to reduce the expression levels of doublecortin (DCX) with short-hairpin (Sh) RNA, we investigated the consequences of impairing migration on the fate of postnatal-formed neurons. First, we showed that electroporation of Dcx ShRNA plasmid efficiently knocks down the expression of DCX and disrupts cells migration along the RMS. Second, we found misplaced anomalous migrating cells that displayed defects in polarity and directionality. Third, patch-clamp recordings performed at 5-7 days post-electroporation (dpe) revealed increased density of voltage-dependent Na(+) channels and enhanced responsiveness to GABA(A) receptor agonist. At later time points (i.e., 12 and 30 dpe), most of the Dcx ShRNA(+) cells developed in the core of the OB and displayed aberrant dendritic length and branching. Additional analysis revealed the formation of GABAergic and glutamatergic synaptic inputs on the mispositioned neurons. Finally, quantifying fate determination by numbering the proportion of GFP(+)/calretinin(+) newborn neurons revealed that Dcx ShRNA(+) cells acquire mature phenotype despite their immature location. We conclude that altering the pace of migration at early stages of postnatal neurogenesis profoundly modifies the tightly orchestrated steps of neuronal maturation, and unveils the influence of microenvironment on controlling neuronal development in the postnatal forebrain.
Assuntos
Movimento Celular/fisiologia , Neurogênese/fisiologia , Neurônios/fisiologia , Bulbo Olfatório/crescimento & desenvolvimento , Animais , Animais Recém-Nascidos , Proteínas do Domínio Duplacortina , Proteína Duplacortina , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Associadas aos Microtúbulos/deficiência , Proteínas Associadas aos Microtúbulos/genética , Neurônios/citologia , Neuropeptídeos/deficiência , Neuropeptídeos/genética , Bulbo Olfatório/citologiaRESUMO
Subcortical heterotopias are malformations associated with epilepsy and intellectual disability, characterized by the presence of ectopic neurons in the white matter. Mouse and human heterotopia mutations were identified in the microtubule-binding protein Echinoderm microtubule-associated protein-like 1, EML1. Further exploring pathological mechanisms, we identified a patient with an EML1-like phenotype and a novel genetic variation in DLGAP4. The protein belongs to a membrane-associated guanylate kinase family known to function in glutamate synapses. We showed that DLGAP4 is strongly expressed in the mouse ventricular zone (VZ) from early corticogenesis, and interacts with key VZ proteins including EML1. In utero electroporation of Dlgap4 knockdown (KD) and overexpression constructs revealed a ventricular surface phenotype including changes in progenitor cell dynamics, morphology, proliferation and neuronal migration defects. The Dlgap4 KD phenotype was rescued by wild-type but not mutant DLGAP4. Dlgap4 is required for the organization of radial glial cell adherens junction components and actin cytoskeleton dynamics at the apical domain, as well as during neuronal migration. Finally, Dlgap4 heterozygous knockout (KO) mice also show developmental defects in the dorsal telencephalon. We hence identify a synapse-related scaffold protein with pleiotropic functions, influencing the integrity of the developing cerebral cortex.
Assuntos
Lissencefalias Clássicas e Heterotopias Subcorticais em Banda , Proteínas Associadas SAP90-PSD95/metabolismo , Animais , Movimento Celular/genética , Córtex Cerebral/metabolismo , Lissencefalias Clássicas e Heterotopias Subcorticais em Banda/metabolismo , Lissencefalias Clássicas e Heterotopias Subcorticais em Banda/patologia , Humanos , Camundongos , Camundongos Knockout , Neurogênese/genética , Neurônios/fisiologiaRESUMO
Radial glial cells (RGCs) in the developing cerebral cortex are progenitors for neurons and glia, and their processes serve as guideposts for migrating neurons. So far, it has remained unclear whether RGC processes also control the function of RGCs more directly. Here, we show that RGC numbers and cortical size are reduced in mice lacking beta1 integrins in RGCs. TUNEL stainings and time-lapse video recordings demonstrate that beta1-deficient RGCs processes detach from the meningeal basement membrane (BM) followed by apoptotic death of RGCs. Apoptosis is also induced by surgical removal of the meninges. Finally, mice lacking the BM components laminin alpha2 and alpha4 show defects in the attachment of RGC processes at the meninges, a reduction in cortical size, and enhanced apoptosis of RGC cells. Our findings demonstrate that attachment of RGC processes at the meninges is important for RGC survival and the control of cortical size.
Assuntos
Córtex Cerebral/anormalidades , Meninges/fisiologia , Neuroglia/fisiologia , Transdução de Sinais/fisiologia , Animais , Animais Recém-Nascidos , Bromodesoxiuridina/metabolismo , Diferenciação Celular/genética , Proliferação de Células , Sobrevivência Celular/genética , Córtex Cerebral/crescimento & desenvolvimento , Eletroporação/métodos , Embrião de Mamíferos , Proteínas do Olho/metabolismo , Regulação da Expressão Gênica no Desenvolvimento/genética , Proteínas de Fluorescência Verde/genética , Proteínas de Homeodomínio/metabolismo , Marcação In Situ das Extremidades Cortadas/métodos , Técnicas In Vitro , Integrases/genética , Integrina beta1/genética , Proteínas de Filamentos Intermediários/deficiência , Laminina/genética , Meninges/citologia , Camundongos , Camundongos Transgênicos , Proteínas do Tecido Nervoso/deficiência , Nestina , Neurogênese/fisiologia , Fator de Transcrição PAX6 , Fatores de Transcrição Box Pareados/metabolismo , Proteínas Repressoras/metabolismo , Transdução de Sinais/genética , Células-Tronco/metabolismoRESUMO
Ongoing neurogenesis maintains neuronal replacement in a few regions of the mammalian adult brain. One of these regions, the subventricular zone, generates olfactory bulb interneuron precursors that must migrate through the rostral migratory stream to reach the olfactory bulb circuit. There, they rapidly initiate dendritic growth and establish dendro-dendritic contacts with mitral/tufted cells and potentially other local interneurons. The sequential steps involved in neuroblast maturation during development have been studied extensively over previous years. However, the mechanisms and regulatory factors controlling the recruitment and first steps of synaptic integration of newly-formed neurons in the adult forebrain have only recently started to be elucidated. This review provides an integrated view of our current understanding of fate-choice decision in progenitors, how newborn neurons correctly migrate to specific circuits, how they integrate in olfactory bulb microcircuits, and the function they have to fulfill once they survive. The elucidation of these mechanisms may be crucial to understand the functional role of adult neurogenesis and eventually develop therapeutic strategies aimed at re-routing neuroblasts to altered circuits.
Assuntos
Movimento Celular/fisiologia , Neurogênese/fisiologia , Neurônios/fisiologia , Células-Tronco/fisiologia , Animais , Animais Recém-Nascidos , Diferenciação Celular/fisiologia , Humanos , Modelos BiológicosRESUMO
[This corrects the article DOI: 10.3389/fncel.2019.00381.].
RESUMO
The development of the cerebral cortex relies on different types of progenitor cell. Among them, the recently described basal radial glial cell (bRG) is suggested to be of critical importance for the development of the brain in gyrencephalic species. These cells are highly numerous in primate and ferret brains, compared to lissencephalic species such as the mouse in which they are few in number. Their somata are located in basal subventricular zones in gyrencephalic brains and they generally possess a basal process extending to the pial surface. They sometimes also have an apical process directed toward the ventricular surface, similar to apical radial glial cells (aRGs) from which they are derived, and whose somata are found more apically in the ventricular zone. bRGs share similarities with aRGs in terms of gene expression (SOX2, PAX6, and NESTIN), whilst also expressing a range of more specific genes (such as HOPX). In primate brains, bRGs can divide multiple times, self-renewing and/or generating intermediate progenitors and neurons. They display a highly specific cytokinesis behavior termed mitotic somal translocation. We focus here on recently identified molecular mechanisms associated with the generation and amplification of bRGs, including bRG-like cells in the rodent. These include signaling pathways such as the FGF-MAPK cascade, SHH, PTEN/AKT, PDGF pathways, and proteins such as INSM, GPSM2, ASPM, TRNP1, ARHGAP11B, PAX6, and HIF1α. A number of these proteins were identified through transcriptome comparisons in human aRGs vs. bRGs, and validated by modifying their activities or expression levels in the mouse. This latter experiment often revealed enhanced bRG-like cell production, even in some cases generating folds (gyri) on the surface of the mouse cortex. We compare the features of the identified cells and methods used to characterize them in each model. These important data converge to indicate pathways essential for the production and expansion of bRGs, which may help us understand cortical development in health and disease.
RESUMO
The subventricular zone (SVZ) of the lateral ventricle is the major site of neurogenesis in the adult brain. Neuroblasts that are born in the SVZ migrate as chains along the rostral migratory stream (RMS) to the olfactory bulb. Little is known about the mechanisms that control interactions between neuroblasts during their migration. Here we show that migrating neuroblasts express beta1 integrins and that the integrin ligand laminin is localized to cell chains. Using genetically modified mice and time-lapse video recordings of SVZ explants, we demonstrate that beta1 integrins and laminin promote the formation of cell chains. Laminin also induces the aggregation of purified neuroblasts. We conclude that the formation of cell chains in the RMS is controlled in part by beta1 integrins via binding to laminin. In addition, we provide evidence that beta1 class integrins are required for the maintenance of the glial tubes and that defects in the glial tubes lead to the ectopic migration of neuroblasts into the surrounding tissue.
Assuntos
Movimento Celular/fisiologia , Integrina beta1/fisiologia , Ventrículos Laterais/citologia , Neurônios/fisiologia , Bulbo Olfatório , Animais , Agregação Celular/fisiologia , Cadeias beta de Integrinas/metabolismo , Integrina beta1/genética , Integrina beta1/metabolismo , Laminina/metabolismo , Laminina/fisiologia , Ventrículos Laterais/patologia , Camundongos , Camundongos Knockout , Neuroglia/fisiologia , Neurônios/patologia , Bulbo Olfatório/patologia , Tamanho do ÓrgãoRESUMO
Radial glial cells in the cerebral cortex serve as progenitors for neurons and glia and guide the migration of cortical neurons. The integrin alpha3beta1 is thought to mediate interactions of migrating neurons with radial glial cells and to function as a receptor for the reelin signaling molecule. Here, we challenge this view and demonstrate that beta1 integrins in migrating neurons are not essential for the formation of cell layers in the cerebral cortex. Cortical cell layers also form normally in mice deficient in the integrin alpha3beta1. However, we provide evidence that beta1 integrins in radial glia control the morphological differentiation of both glia and neurons. We conclude that beta1 integrins in radial glia are required for the proper development of the cerebral cortex, whereas beta1 integrins in migrating neurons are not essential for glial-guided migration and reelin signaling.
Assuntos
Córtex Cerebral/embriologia , Integrina beta1/fisiologia , Neuroglia/metabolismo , Neurônios/fisiologia , Animais , Animais Recém-Nascidos , Contagem de Células , Diferenciação Celular/genética , Diferenciação Celular/fisiologia , Movimento Celular/genética , Movimento Celular/fisiologia , Células Cultivadas , Córtex Cerebral/citologia , Córtex Cerebral/metabolismo , Cruzamentos Genéticos , Regulação da Expressão Gênica no Desenvolvimento , Marcação de Genes/métodos , Integrases/genética , Integrina alfa3beta1/genética , Integrina alfa3beta1/fisiologia , Integrina beta1/genética , Proteínas de Filamentos Intermediários/genética , Camundongos , Camundongos Mutantes Neurológicos , Camundongos Transgênicos , Proteínas do Tecido Nervoso/genética , Nestina , Neuroglia/citologia , Neurônios/citologia , Proteína ReelinaRESUMO
Brain development involves extensive migration of neurons. Microtubules (MTs) are key cellular effectors of neuronal displacement that are assembled from α/ß-tubulin heterodimers. Mutation of the α-tubulin isotype TUBA1A is associated with cortical malformations in humans. In this study, we provide detailed in vivo and in vitro analyses of Tuba1a mutants. In mice carrying a Tuba1a missense mutation (S140G), neurons accumulate, and glial cells are dispersed along the rostral migratory stream in postnatal and adult brains. Live imaging of Tuba1a-mutant neurons revealed slowed migration and increased neuronal branching, which correlated with directionality alterations and perturbed nucleus-centrosome (N-C) coupling. Tuba1a mutation led to increased straightness of newly polymerized MTs, and structural modeling data suggest a conformational change in the α/ß-tubulin heterodimer. We show that Tuba8, another α-tubulin isotype previously associated with cortical malformations, has altered function compared with Tuba1a. Our work shows that Tuba1a plays an essential, noncompensated role in neuronal saltatory migration in vivo and highlights the importance of MT flexibility in N-C coupling and neuronal-branching regulation during neuronal migration.
Assuntos
Encéfalo/metabolismo , Movimento Celular , Microtúbulos/metabolismo , Neurogênese , Neurônios/metabolismo , Tubulina (Proteína)/metabolismo , Animais , Encéfalo/patologia , Núcleo Celular/metabolismo , Centrossomo/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Genótipo , Camundongos Endogâmicos C3H , Camundongos Mutantes , Microscopia de Fluorescência , Microtúbulos/patologia , Simulação de Dinâmica Molecular , Mutação de Sentido Incorreto , Neuroglia/metabolismo , Neuroglia/patologia , Neurônios/patologia , Fenótipo , Multimerização Proteica , Estrutura Quaternária de Proteína , Transdução de Sinais , Relação Estrutura-Atividade , Fatores de Tempo , Imagem com Lapso de Tempo , Tubulina (Proteína)/química , Tubulina (Proteína)/genética , Gravação em VídeoRESUMO
mCD24, a glycosylphosphatidylinositol-anchored highly glycosylated molecule, is expressed on differentiating neurons during development. In the adult CNS, its expression is restricted to immature neurons located in two regions showing ongoing neurogenesis: the subventricular zone (SVZ) of the lateral ventricle pathway and the dentate gyrus (DG) of the hippocampal formation. Here, combining bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen labelings we confirmed that mCD24 is expressed on proliferating cells. To determine whether the inactivation of the molecule may affect adult neurogenesis, we analyzed the phenotype of mCD24-deficient mice (mCD24-/-). We labeled cells in S-phase with a pulse, a long, or a cumulative administration of BrdU and analyzed cells in different zones according to their dividing rate (rapid and slow) both in the control and mCD24-/-. We found a significant increase in the number of rapid (in the SVZ and the DG) and slow (in the SVZ) proliferating cells. Cumulative assays revealed a global reduction of the total cell cycle duration of rapidly proliferating precursors of SVZ. We investigated the fate of supernumerary cells and observed an increased number of apoptotic cells (terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling) in the mutant SVZ. Furthermore, we found no difference in the size of the olfactory bulb between wild-type (WT) and mutant mice. In support, mCD24 deletion did not appear to affect migration in the migratory stream. A comparison of the organization of migrating precursors between WT and mCD24 -/-, both in vivo at the optic and electron microscopic levels and in SVZ cultured explants, did not show any changes in the arrangement of neuroblasts in chain-like structures. Altogether, our data suggest that mCD24 regulates negatively cell proliferation in zones of secondary neurogenesis.
Assuntos
Antígenos CD/genética , Glicoproteínas de Membrana , Neurônios/citologia , Animais , Antígenos CD/metabolismo , Apoptose , Bromodesoxiuridina/farmacocinética , Antígeno CD24 , Contagem de Células , Ciclo Celular/genética , Diferenciação Celular/genética , Divisão Celular/genética , Movimento Celular/genética , Giro Denteado/citologia , Giro Denteado/metabolismo , Marcação In Situ das Extremidades Cortadas , Ventrículos Laterais/citologia , Ventrículos Laterais/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Neurônios/metabolismo , Bulbo Olfatório/citologia , Fenótipo , Antígeno Nuclear de Célula em Proliferação/biossíntese , Células-Tronco/citologiaRESUMO
We have previously shown that mice with a CNS restricted knock-out of the integrin beta1 subunit gene (Itgb1-CNSko mice) have defects in the formation of lamina and folia in the cerebral and cerebellar cortices that are caused by disruption of the cortical marginal zones. Cortical structures in postnatal and adult Itgb1-CNSko animals are also reduced in size, but the mechanism that causes the size defect has remained unclear. We now demonstrate that proliferation of granule cell precursors (GCPs) is severely affected in the developing cerebellum of Itgb1-CNSko mice. In the absence of beta1 expression, GCPs lose contact with laminin in the meningeal basement membrane, cease proliferating, and differentiate prematurely. In vitro studies provide evidence that beta1 integrins act at least in part cell autonomously in GCPs to regulate their proliferation. Previous studies have shown that sonic hedgehog (Shh)-induced GCP proliferation is potentiated by the integrin ligand laminin. We show that Shh directly binds to laminin and that laminin-Shh induced cell proliferation is dependent on beta1 integrin expression in GCPs. Taken together, these data are consistent with a model in which beta1 integrin expression in GCPs is required to recruit a laminin-Shh complex to the surface of GCPs and to subsequently modulate the activity of signaling pathways that regulate proliferation.
Assuntos
Cerebelo/citologia , Integrina beta1/fisiologia , Neurônios/fisiologia , Células-Tronco/citologia , Células-Tronco/fisiologia , Animais , Proteínas de Ciclo Celular/metabolismo , Divisão Celular/efeitos dos fármacos , Divisão Celular/genética , Divisão Celular/fisiologia , Células Cultivadas , Cerebelo/embriologia , Cerebelo/crescimento & desenvolvimento , Inibidor de Quinase Dependente de Ciclina p27 , Matriz Extracelular/fisiologia , Regulação da Expressão Gênica no Desenvolvimento , Marcação de Genes/métodos , Substâncias de Crescimento/farmacologia , Substâncias de Crescimento/fisiologia , Proteínas Hedgehog , Hibridização In Situ , Integrases , Integrina beta1/genética , Integrina beta1/farmacologia , Laminina/metabolismo , Camundongos , Camundongos Knockout , Camundongos Transgênicos , Neurônios/citologia , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia , Células-Tronco/efeitos dos fármacos , Transativadores/metabolismo , Transativadores/farmacologia , Proteínas Supressoras de Tumor/metabolismo , Proteínas ViraisRESUMO
In this review, we focus on CA3 neuronal migration disorders in the rodent. We begin by introducing the main steps of hippocampal development, and we summarize characteristic hippocampal malformations in human. We then describe various mouse mutants showing structural hippocampal defects. Notably, genes identified in human cortical neuronal migration disorders consistently give rise to a CA3 phenotype when mutated in the mouse. We successively describe their molecular, physiological and behavioral phenotypes that together contribute to a better understanding of CA3-dependent functions. We finally discuss potential factors underlying the CA3 vulnerability revealed by these mouse mutants and that may also contribute to other human neurological and psychiatric disorders.
RESUMO
Neuronal migration disorders such as lissencephaly and subcortical band heterotopia are associated with epilepsy and intellectual disability. DCX, PAFAH1B1 and TUBA1A are mutated in these disorders; however, corresponding mouse mutants do not show heterotopic neurons in the neocortex. In contrast, spontaneously arisen HeCo mice display this phenotype, and our study revealed that misplaced apical progenitors contribute to heterotopia formation. While HeCo neurons migrated at the same speed as wild type, abnormally distributed dividing progenitors were found throughout the cortical wall from embryonic day 13. We identified Eml1, encoding a microtubule-associated protein, as the gene mutated in HeCo mice. Full-length transcripts were lacking as a result of a retrotransposon insertion in an intron. Eml1 knockdown mimicked the HeCo progenitor phenotype and reexpression rescued it. We further found EML1 to be mutated in ribbon-like heterotopia in humans. Our data link abnormal spindle orientations, ectopic progenitors and severe heterotopia in mouse and human.