1.
STAR Protoc
; 5(3): 103256, 2024 Aug 13.
Artigo
em Inglês
| MEDLINE
| ID: mdl-39141519
RESUMO
Extracellular vesicles (EVs) are secreted, cell-derived, membrane-bound compartments implicated in various diseases for their ability to influence distant targets and as carriers of biomarkers. Here, we present a protocol for separating EVs from mammalian pancreatic cancer cells and their characterization using western blot and electron microscopy. We then demonstrate how they are utilized to affect tumor development in a murine model of metastatic pancreatic cancer including a method to quantify hepatic tumor burden in histologic samples. For complete details on the use and execution of this protocol, please refer to Dudgeon et al.1.
2.
Med Sci (Paris)
; 40(2): 124-126, 2024 Feb.
Artigo
em Francês
| MEDLINE
| ID: mdl-38411414