Effect of Shade on Agro-Morphological Parameters and Weed Flora of Saffron (Crocus sativus L.) Cultivation in the Semiarid Zone of Eastern Morocco.

Saffron (Crocus sativus) has been an important medicinal plant since ancient times. This study aimed to seek the optimal light intensity for saffron growth by quantifying the effects of different shade levels on yield, vegetative growth, and weed development in the eastern region of Morocco. The plants were grown for 24 months in full sun (control) and 30%, 50%, and 70% shade. Overall, the results showed that shade positively affected the yield and vegetative growth parameters of saffron plants, with the highest yield (0.61 g/m2) and number of leaves (105 leaves/tuft) recorded when the plants were exposed to light shade (30%). The color of the leaves under the 70% shade levels was dark green. The results from the underground part showed that shade is positively correlated with the weight and diameter of daughter corms where the 70% shade recorded the highest values of weight (65 g) and percentage of large diameter corms (39%). As for weed density, this parameter was significantly affected by shade. The lowest weed density was recorded for the 70% shade treatment. In conclusion, 30% shade is suggested as optimal light irradiation for saffron cultivation.

Study of the Effect of Mulching Materials on Weed Control in Saffron Cultivation in Eastern Morocco.

Saffron (Crocus sativus L.) is cultivated in many countries for its culinary and medicinal values. The production of saffron is limited by several factors, including weed infestation, which causes damage to the crop in terms of quantity and quality. However, little information is available on the different weed management strategies for saffron cultivation, as most of the strategies implemented are developed for large-scale and conventional agriculture. As a result, they are not applicable or affordable for organic or smallholder farmers, as is the case for saffron cultivation. The objective of this study is to compare the effectiveness of plastic mulching versus mulching in controlling weeds in saffron cultivation in the eastern region of Morocco. During the trial, which was conducted in 2018, the parameters measured correspond, on the one hand, to morphometric measurements and determination of saffron stigma yield and, on the other hand, to the determination of density, dry biomass, and weed control capacity. Compared to the control, mulching reduced the population and dry biomass of the most formidable weeds such as Cynodon dactylon, Aster squamatus, Cyperus rotundus, and Convolvulus arvensis. The average stigmata yield from plastic mulch treatment was 9% higher than of the control, and the number of leaves, leaf area, number, weight, and percentage of daughter corms with large diameter were higher for plants grown under mulch. Overall, the results of this study showed that the use of PE (polyethylene) mulch effectively reduced weed populations and improved saffron yield and vegetative growth.

Exploring the Multi-Faceted Potential of Carob (Ceratonia siliqua var. Rahma) Leaves from Morocco: A Comprehensive Analysis of Polyphenols Profile, Antimicrobial Activity, Cytotoxicity against Breast Cancer Cell Lines, and Genotoxicity.

The botanical species Ceratonia siliqua L., commonly referred to as the Carob tree, and locally as "L'Kharrûb", holds significance as an agro-sylvo-pastoral species, and is traditionally utilized in Morocco for treating a variety of ailments. This current investigation aims to ascertain the antioxidant, antimicrobial, and cytotoxic properties of the ethanolic extract of C. siliqua leaves (CSEE). Initially, we analyzed the chemical composition of CSEE through high-performance liquid chromatography with Diode-Array Detection (HPLC-DAD). Subsequently, we conducted various assessments, including DPPH scavenging capacity, ß-carotene bleaching assay, ABTS scavenging, and total antioxidant capacity assays to evaluate the antioxidant activity of the extract. In this study, we investigated the antimicrobial properties of CSEE against five bacterial strains (two gram-positive, Staphylococcus aureus, and Enterococcus faecalis; and three gram-negative bacteria, Escherichia coli, Escherichia vekanda, and Pseudomonas aeruginosa) and two fungi (Candida albicans, and Geotrichum candidum). Additionally, we evaluated the cytotoxicity of CSEE on three human breast cancer cell lines (MCF-7, MDA-MB-231, and MDA-MB-436) and assessed the potential genotoxicity of the extract using the comet assay. Through HPLC-DAD analysis, we determined that phenolic acids and flavonoids were the primary constituents of the CSEE extract. The results of the DPPH test indicated a potent scavenging capacity of the extract with an IC50 of 302.78 ± 7.55 µg/mL, which was comparable to that of ascorbic acid with an IC50 of 260.24 ± 6.45 µg/mL. Similarly, the ß-carotene test demonstrated an IC50 of 352.06 ± 12.16 µg/mL, signifying the extract's potential to inhibit oxidative damage. The ABTS assay revealed IC50 values of 48.13 ± 3.66 TE µmol/mL, indicating a strong ability of CSEE to scavenge ABTS radicals, and the TAC assay demonstrated an IC50 value of 165 ± 7.66 µg AAE/mg. The results suggest that the CSEE extract had potent antioxidant activity. Regarding its antimicrobial activity, the CSEE extract was effective against all five tested bacterial strains, indicating its broad-spectrum antibacterial properties. However, it only showed moderate activity against the two tested fungal strains, suggesting it may not be as effective against fungi. The CSEE exhibited a noteworthy dose-dependent inhibitory activity against all the tested tumor cell lines in vitro. The extract did not induce DNA damage at the concentrations of 6.25, 12.5, 25, and 50 µg/mL, as assessed by the comet assay. However, the 100 µg/mL concentration of CSEE resulted in a significant genotoxic effect compared to the negative control. A computational analysis was conducted to determine the physicochemical and pharmacokinetic characteristics of the constituent molecules present in the extract. The Prediction of Activity Spectra of Substances (PASS) test was employed to forecast the potential biological activities of these molecules. Additionally, the toxicity of the molecules was evaluated using the Protox II webserver.

Hypotensive property of Chenopodium ambrosioides in anesthetized normotensive rats.

BACKGROUND: The leaves of Chenopodium ambrosioides L. (Chenopodiaceae) are widely used in Moroccan traditional medicine to treat diabetes and hypertension. The goal of the present work is to investigate the hypotensive properties of different extract and fractions of the plant in anesthetized normotensive rats and to elucidate the mechanism underlying this effect. METHODS: The hypotensive effect of aqueous extract (AqE) of the leaves of C. ambrosioides L., methanolic (MF), ethyl acetate (AcF), and aqueous (AqF) Soxhlet fractions, administrated intravenously, was evaluated in anesthetized rats. The recorded signals of blood pressure and heart rate were visualized and analyzed by using an acquisition card "National Instrument" and software Labview 6.1. RESULTS: Intravenous administration of AqE of the leaves of C. ambrosioides L. induces a dose-dependent hypotension. A similar effect was obtained with MF, AcF, and AqF. Atropine (1 mg/kg), used to block cholinergic system, significantly reduced the hypotensive response to MF and AcF suggesting the presence of the cholinomimetic-muscarinic components in these fractions. However, the blood pressure lowering effect of MF and AcF in rats pretreated with L-NAME 20 mg/kg was unchanged showing that the release of NO is not implicated in the hypotensive action of this plant. CONCLUSIONS: The present study demonstrates that extracts from leaves of C. ambrosioides induce hypotensive effect that may be partially associated with its cardiac effects. These results may partly explain the traditional use of leaves of C. ambrosioides L. for the treatment of disorders such as hypertension.