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1.
Am J Physiol Gastrointest Liver Physiol ; 312(6): G658-G665, 2017 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-28209602

RESUMO

Nonerosive reflux disease (NERD) is a highly prevalent phenotype of the gastroesophageal reflux disease. In this study, we developed a novel murine model of NERD in mice with microscopic inflammation and impairment in the epithelial esophageal barrier. Female Swiss mice were subjected to the following surgical procedure: the transitional region between the forestomach and the glandular portion of the stomach was ligated, and a nontoxic ring was placed around the duodenum near the pylorus. The control group underwent sham surgery. The animals were euthanized at 1, 3, 7, and 14 days after surgery. Survival and body weight were monitored daily. Esophageal wet weight, macroscopic lesion, histopathological alterations, myeloperoxidase (MPO) activity, cytokine levels, transepithelial electrical resistance (TEER), and mucosal permeability were evaluated. The survival rate was 78% at 14 days, with mild loss in body weight. Surgery did not induce erosive esophagitis but instead induced microscopic inflammation and increased esophageal wet weight, IL-6, keratinocyte-derived cytokine (KC) levels, and MPO activity with maximal peak between 3 and 7 days and resolution at 14 days postsurgery. Epithelial esophageal barrier was evaluated in operated mice at 7 and 14 days postsurgery; a decrease in TEER and increase in the esophageal epithelial permeability were observed compared with the sham-operated group. In addition, the inhibition of acid secretion with omeprazole significantly prevented the esophageal inflammation and impairment of barrier function at 7 days postsurgery. Thus we established a novel experimental model of NERD in mice, which can contribute to understanding the pathophysiological events associated with NERD.NEW & NOTEWORTHY In this study, we standardized an experimental model of nonerosive reflux disease (NERD) in mice. This model involves an acute inflammatory response followed by impaired esophageal mucosal integrity, even in the absence of inflammation. Thus this model can serve for evaluation of pathophysiological aspects of NERD and open new perspectives for therapeutic strategies for patients with this disorder.


Assuntos
Mucosa Esofágica/patologia , Esofagite Péptica/patologia , Refluxo Gastroesofágico/patologia , Animais , Citocinas/metabolismo , Modelos Animais de Doenças , Duodeno/cirurgia , Impedância Elétrica , Mucosa Esofágica/efeitos dos fármacos , Mucosa Esofágica/metabolismo , Mucosa Esofágica/fisiopatologia , Esofagite Péptica/etiologia , Esofagite Péptica/metabolismo , Esofagite Péptica/fisiopatologia , Feminino , Refluxo Gastroesofágico/etiologia , Refluxo Gastroesofágico/metabolismo , Refluxo Gastroesofágico/fisiopatologia , Mediadores da Inflamação/metabolismo , Ligadura , Camundongos , Tamanho do Órgão , Permeabilidade , Peroxidase/metabolismo , Fenótipo , Inibidores da Bomba de Prótons/farmacologia , Estômago/cirurgia , Fatores de Tempo
2.
Carbohydr Polym ; 152: 140-148, 2016 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-27516258

RESUMO

A sulfated polysaccharide (SFP) fraction from the marine alga Solieria filiformis was extracted and submitted to microanalysis, molar mass estimation and spectroscopic analysis. We evaluated its gastroprotective potential in vivo in an ethanol-induced gastric damage model and its in vitro antioxidant properties (DPPH, chelating ferrous ability and total antioxidant capacity). Its chemical composition revealed to be essentially an iota-carrageenan with a molar mass of 210.9kDa and high degree of substitution for sulfate groups (1.08). In vivo, SFP significantly (P<0.05) reduced, in a dose dependent manner, the ethanol-induced gastric damage. SFP prevents glutathione consume and increase of malondialdehyde and hemoglobin levels. SFP presented an IC50 of 1.77mg/mL in scavenging DPPH. The chelating ferrous ability was 38.98%, and the total antioxidant capacity was 2.01mg/mL. Thus, SFP prevents the development of ethanol-induced gastric damage by reducing oxidative stress in vivo and possesses relevant antioxidant activity in vitro.


Assuntos
Antioxidantes , Estresse Oxidativo/efeitos dos fármacos , Polissacarídeos , Rodófitas/química , Gastropatias/prevenção & controle , Animais , Antioxidantes/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Etanol/toxicidade , Camundongos , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Gastropatias/induzido quimicamente
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