Octopus vulgaris Exhibits Interindividual Differences in Behavioural and Problem-Solving Performance.

By presenting individual Octopus vulgaris with an extractive foraging problem with a puzzle box, we examined the possible correlation between behavioural performances (e.g., ease of adaptation to captive conditions, prevalence of neophobic and neophilic behaviours, and propensity to learn individually or by observing conspecifics), biotic (body and brain size, age, sex) and abiotic (seasonality and place of origin) factors. We found more neophilic animals showing shorter latencies to approach the puzzle box and higher probability of solving the task; also, shorter times to solve the task were correlated with better performance on the individual learning task. However, the most neophilic octopuses that approached the puzzle box more quickly did not reach the solution earlier than other individuals, suggesting that strong neophilic tendency may lead to suboptimal performance at some stages of the problem-solving process. In addition, seasonal and environmental characteristics of location of origin appear to influence the rate of expression of individual traits central to problem solving. Overall, our analysis provides new insights into the traits associated with problem solving in invertebrates and highlights the presence of adaptive mechanisms that promote population-level changes in octopuses' behavioural traits.

Non-Invasive Assessment of the Seasonal Stress Response to Veterinary Procedures and Transportation of Zoo-Housed Lesser Anteater (Tamandua tetradactyla).

Management procedures affect behavioural and physiological stress responses of wild mammals under human care. According to the Reactive Scope Model, normal values are presumed to exist within predictive and reactive ranges. First, stress parameters of zoo-housed adult Tamandua tetradactyla were evaluated in winter and summer (29 days each), determining the level of behaviour and/or physiological parameters needed to respond to predictable environmental changes. Secondly, the effects of veterinary procedures and transportation were studied in both seasons. Non-invasive methods were applied, assessing behaviour through videos and adrenocortical activity by faecal glucocorticoid metabolites (FGMs). Lesser anteaters exhibited seasonality (summer > winter) in some behavioural parameters, such as nocturnal activities, as well as in the activity cycle (e.g., acrophase) and FGMs. A veterinary check elicited an increase in total activity (TA), natural behaviours and repetitive locomotion and affected the activity cycle, particularly in summer. Transport produced changes in TA, nocturnal and natural activity and some variables of the activity cycle, mostly during summer. Although the effects of routine management procedures were different from each other and presumably stressful, they elicited changes only at the behavioural level, which was greater during summer. The differences observed according to non-invasive methodologies highlight the importance of a multidisciplinary approach in this context and suggest that it is unlikely that individual welfare was affected.

A standardized battery of tests to measure Octopus vulgaris' behavioural performance.

Here we introduce a series of behavioural tasks to assess inter-individual variability in behaviours exhibited by the cephalopod mollusc Octopus vulgaris. We propose that, by using octopus' predatory behavioural response, it is possible to measure: (1) the ability to adapt to the captive condition (acclimatization), (2) the response towards novel stimuli (neophobia), (3) the capability of social learning, (4) the ability of solving problems (problem solving), and (5) the response to artificial stimuli (preferences, individual learning). To assure comparability and reproducibility of results, this battery of tests is here applied to a large sample of individuals in standardized experimental conditions. Such battery of tests serves as an in vivo screening that should be adopted not only to investigate cognitive abilities in specific behavioural domains, but also to monitor the welfare status of animals under captivity, thus to check sensory functions as well as motor abilities in other investigations within the fields of biology and neuroscience. Our aim was to provide a reliable tool to exploit this animal species for research in different fields.

Cerebrotypes in Cephalopods: Brain Diversity and Its Correlation With Species Habits, Life History, and Physiological Adaptations.

Here we analyze existing quantitative data available for cephalopod brains based on classical contributions by J.Z. Young and colleagues, to cite some. We relate the relative brain size of selected regions (area and/or lobe), with behavior, life history, ecology and distribution of several cephalopod species here considered. After hierarchical clustering we identify and describe ten clusters grouping 52 cephalopod species. This allows us to describe cerebrotypes, i.e., differences of brain composition in different species, as a sign of their adaptation to specific niches and/or clades in cephalopod molluscs for the first time. Similarity reflecting niche type has been found in vertebrates, and it is reasonable to assume that it could also occur in Cephalopoda. We also attempted a phylogenetic PCA using data by Lindgren et al. (2012) as input tree. However, due to the limited overlap in species considered, the final analysis was carried out on <30 species, thus reducing the impact of this approach. Nevertheless, our analysis suggests that the phylogenetic signal alone cannot be a justification for the grouping of species, although biased by the limited set of data available to us. Based on these preliminary findings, we can only hypothesize that brains evolved in cephalopods on the basis of different factors including phylogeny, possible development, and the third factor, i.e., life-style adaptations. Our results support the working hypothesis that the taxon evolved different sensorial and computational strategies to cope with the various environments (niches) occupied in the oceans. This study is novel for invertebrates, to the best of our knowledge.

I know my neighbour: individual recognition in Octopus vulgaris.

BACKGROUND: Little is known about individual recognition (IR) in octopuses, although they have been abundantly studied for their sophisticated behaviour and learning capacities. Indeed, the ability of octopuses to recognise conspecifics is suggested by a number of clues emerging from both laboratory studies (where they appear to form and maintain dominance hierarchies) and field observations (octopuses of neighbouring dens display little agonism between each other). To fill this gap in knowledge, we investigated the behaviour of 24 size-matched pairs of Octopus vulgaris in laboratory conditions. METHODOLOGY/PRINCIPAL FINDINGS: The experimental design was composed of 3 phases: Phase 1 (acclimatization): 12 "sight-allowed" (and 12 "isolated") pairs were maintained for 3 days in contiguous tanks separated by a transparent (and opaque) partition to allow (and block) the vision of the conspecific; Phase 2 (cohabitation): members of each pair (both sight-allowed and isolated) were transferred into an experimental tank and were allowed to interact for 15 min every day for 3 consecutive days; Phase 3 (test): each pair (both sight-allowed and isolated) was subject to a switch of an octopus to form pairs composed of either familiar ("sham switches") or unfamiliar conspecifics ("real switches"). Longer latencies (i.e. the time elapsed from the first interaction) and fewer physical contacts in the familiar pairs as opposed to the unfamiliar pairs were used as proxies for recognition. CONCLUSIONS: Octopuses appear able to recognise conspecifics and to remember the individual previously met for at least one day. To the best of our knowledge, this is the first experimental study showing the occurrence of a form of IR in cephalopods. Future studies should clarify whether this is a "true" IR.