Metaproteomic analysis of King Ghezo tomb wall (Abomey, Benin) confirms 19th century voodoo sacrifices.

The palace of King Ghezo in Abomey, capital of the ancient kingdom of Dahomey (present-day Benin), houses two sacred huts which are specific funerary structures. It is claimed that the binder in their walls is made of human blood. In the study presented here, we conceived an original strategy to analyze the proteins present on minute amounts of the cladding sampled from the inner facade of the cenotaph wall and establish their origin. The extracted proteins were proteolyzed and the resulting peptides were characterized by high-resolution tandem mass spectrometry. Over 6397 distinct molecular entities were identified using cascading searches. Starting from without a priori searches of an extended generic database, the peptide repertoire was narrowed down to the most representative organisms-identified by means of taxon-specific peptides. A wide diversity of bacteria, fungi, plants, and animals were detected through the available protein material. This inventory was used to archaeologically reconstruct the voodoo rituals of consecration and maintenance of vitality. Several indicators attested to the presence of traces of human and poultry blood in the material taken. This study shows the essential advantages of paleoproteomics and metaproteomics for the study of ancient residues from archaeological excavations or historical monuments.

Deep Paleoproteotyping and Microtomography Revealed No Heart Defect nor Traces of Embalming in the Cardiac Relics of Blessed Pauline Jaricot.

Scientific examination of the heart of Blessed Pauline Jaricot-a French missionary figure-was carried out in 2022. As tandem mass spectrometry proteotyping has proven to be valuable to obtain the broad taxonomic repertoire of a given sample without any a priori information, we aimed at exploring the conditions of preservation of the relics and possible conditions of death. Metaproteomics and high-resolution microtomography imaging approaches were combined. A dataset comprising 6731 high-resolution MS/MS spectra was acquired and 968 of these spectra could be assigned to specific peptidic biomolecules. Based on the taxonomical information encompassed by the identified peptide sequences, 5 phyla were identified amongst eukaryota (94% of the biomass): Ascomycota (55%), with the species Aspergillus versicolor, Trichophyton mentagrophytes and Aspergillus glaucus, corresponding to expected cadaverous fungal flora; Chordata (42%), represented by a unique species, Homo sapiens; Streptophyta (3%); and Arthropoda (traces). Bacteria (6% of the biomass) were poorly represented. No trace of embalming substance could be retrieved, nor any pathogens. Imaging evidenced no heart defect nor embalming traces. No evidence that was inconsistent with natural and spontaneous conservation could be retrieved. This study prefigures the power of modern molecular techniques such as paleoproteotyping coupled to microtomography to gain insight into historical relics.

The last jump: epidemiology of suicides at the Tour Eiffel (Paris) between 1950 and 1989.

The Eiffel Tower (Paris, France) has been attracting many candidates for suicide over the years. The aim of this study was to determine the patterns of people who committed suicide by jumping. Permission to access the unpublished records of the Paris Police Prefecture made it possible to compile press articles related to suicides from April 1950 to March 1989. 155 articles corresponding to 79 cases were kept for analysis. There were significantly more males (66%), as compared with an expected theoretical distribution of 1/2 for each sex (p = 0.002). Median age was 38.0 years old. Suicide peak was reached during the months of August and October (13% for each), while February was poor in suicides (3%). Tuesdays and Saturdays were the more impacted days. Hourly suicide rates were 8% (morning), 11% (noon), 7% (afternoon), and 5% (evening-night). Moon phases did not correlate with suicide occurrence. The main context described was that of a psychiatric pathology (49%). Death occurred in 92% of the reported cases. Sex ratio, ages, decrease during the cold season, no association with moon phases, and underlying psychiatric pathologies were consistent with trends for either all-cause suicides or suicide jumps from a platform. Conversely, unusual trough in suicides on Mondays and noon peak in suicide rate have been, respectively, attributed to small sample size and closure at night. As jumping corresponds to both ease and proximity, we believe the implementation of protective measures in the early 1960s succeeded in reducing the number of suicides overall.

Tribal makeup painting mimicking achromic lupus in a Teke tribal chef: icono-diagnosis is a dangerous art.

A close-up photograph of a Teke community Chief (Congo, circa 1880-1890) seemed to be of diagnostic interest, as areas of achromic maculae were visible on the hands: the most relevant diagnoses were a Koebner phenomenon on a background of vitiligo, or a diagnosis of lupus. However, examination of the entire photograph showed that the 'maculae' were in fact tribal makeup, showing the methodological limits of icono-diagnosis.

Toxicological analysis of a "poison vial" found in the remains of an SS soldier (Maltot, Normandy, France).

In Maltot (Normandy, France), one grave containing the remains of a German soldier, who died in 1944, was excavated amongst other graves and isolated elements. A dozen whole vials were unearthed, resulting in questions about their content. Various screenings were carried out on the contents of one single vial: HPLC-DAD and HR-LC-MS screening after 1/10 dilution in mobile phase, GC-MS and HS-GC-MS after 1/10 dilution in methanol, multi-element research by HR-ICP-MS after total mineralization, and cyanide analysis. Analyzed vial contained approximately 300 µL of a colorless, water-immiscible liquid with a characteristic solvent odor. HPLC-DAD, GC-MS, HR-LC-MS/MS, ICP-MS, and cyanide screenings were negative excluding the presence of cyanide, arsenic, barbiturates, amphetamines, or narcotics. HS-GC-MS analysis highlighted the presence of ethanol, chloroform, and diethyl ether at significant concentrations. Chloroform and diethyl ether were anesthetic products mainly reserved for urgent situations. We hypothesized that the soldier may have been a combat medic working on battlefields. as he was wounded, another possibility could be that he may have used the vials to relieve his pain; however, the immediate severity of the wounds drove us to assess the second hypothesis of delayed death as being less plausible. The high number of vials containing ethanol, chloroform, and diethyl ether, and the massive blood loss leading to quick death led us to support the combat medic or paramedic hypothesis.

Forensic description of impalement and associated torture lesions by Napoleon troops (Egypt, 1800).

Impalement bone traumas are very rarely described in the medical literature. We here present the first case of such a diagnosis carried out on a skeleton. The patient is Soleyman el-Halaby, executed in June 1800, who was the 24-year-old murderer of the Napoleonic general Kléber. The skeleton is now held in the National Museum of Natural History (Paris). This report describes the traumatic lesions diagnosed after a careful forensic anthropological examination. Such a case is important from a medical point of view, whether the origin of the pelvic or abdominal impalement is accidental or criminal. Forensic examination of skeletons conserved in anthropological collections may help reconstruct the modalities of death, and give data for an eventual repatriation process to the original community.

Evidence of Cushing's syndrome in a pre-Columbian Mexican statue?

INTRODUCTION: In the absence of skeletons or written narrations, information about diseases in past societies may be acquired from icono-diagnosis. From the observation of a masterpiece presenting pathological features, we tried to make retrospective diagnosis. MATERIAL AND METHOD: A pre-Columbian Mexican statuette originating from the Chupicuaro culture and dated 600 BC to 200 AD - conserved in the Louvre Museum in Paris (Section of the quai Branly - Jacques Chirac museum) was examined; it was found to display a huge spinal curvature with excessive dorsal kyphosis and obesity. RESULTS: The appearance of the figurine with large head, shortened stature and limbs, may suggest a form of dwarfism; however, many statuettes in the Chupicuaro culture were found displaying large head and relatively short limbs, suggesting that these pictorial features are more of an artistic style. On the contrary, uncommon kyphosis and obesity led us to diagnose a case of either Pott disease associated with neuro-endocrine complications, or of Cushing's disease. CONCLUSION: Although icono-diagnosis could have allowed us to contribute to the health mapping of ancient Americas and propose the presence of complicated tuberculosis in central Mexico between 600 BC and 200 AD, we believe "Choupi" portrays here an individual having suffered from hypercortisolism (Cushing's disease). Even though considerations related to cultural and artistic context may constitute limitations to interpretation, iconotopsy/iconodiagnosis are important for a better description of the natural history of diseases, as a complement to morphological analyses of human remains (paleopathology) and laboratory exams (DNA or immunology testing).