Preclinical evaluation of an endoscopic electroporation system.

BACKGROUND AND STUDY AIMS: Targeted delivery of specific chemotherapeutic drugs into tumors can be achieved by delivering electrical pulses directly to the tumor tissue. This causes a transient formation of pores in the cell membrane that enables passive diffusion of normally impermeant drugs. A novel device has been developed to enable the endoscopic delivery of this tumor permeabilizing treatment. The aim of the preclinical studies described here was to investigate the efficacy and safety of this nonthermal ablation system in the treatment of gastrointestinal cancer models. METHODS: Murine, porcine, and canine gastrointestinal tumors and tissues were used to assess the efficacy and safety of electroporation delivered through the special device in combination with bleomycin. Tumor cell death, volume, and overall survival were recorded. RESULTS: Murine tumors treated with electrochemotherapy showed excellent responses, with cell death being induced rapidly, mainly via an apoptotic-type mechanism. Use of the system in canine gastrointestinal cancers demonstrated successful local endoluminal tumor resolution, with no safety or adverse effects noted. CONCLUSIONS: Electroporation via the new device in combination with bleomycin offers a nonthermal tumor ablative approach, and presents clinicians with a new option for the management of gastrointestinal cancers.

Graft tensioning practices in anterior cruciate ligament reconstruction amongst orthopaedic surgeons in Australia: a national survey.

PURPOSE: The application of graft tension during anterior cruciate ligament reconstruction is considered an important feature of ACLR. However, wide variation exists in relation to graft tensioning practice limiting the ability to determine the best approach. Thus, the primary aim of this study was to describe current clinical practice amongst Australian orthopaedic surgeons with respect to graft tensioning and explore influencing factors. MATERIALS AND METHODS: A survey was developed to address the aims of the study and pilot testing was completed to confirm validity and reliability. The survey population was defined as Australian orthopaedic surgeons, associated with the Australian Orthopaedic Association sub-specialty of knee to target surgeons likely to perform ACLR. The final sampling frame consisted of 192 surgeons. RESULTS: Manual tensioning was the most common method (80.5 %), with a maximum one-handed pull the most frequent description and estimated tension ranged between 41 and 60 N with the knee positioned near full extension. Surgeons using a tensioning device tended to use a higher tension (mean 81.85 N), with the knee positioned at 30° flexion (40 %). Sixteen percent reported individualising tension on viscoelasticity of the graft, graft diameter, patient anthropometry and age. Patient outcomes and available evidence were the primary factors influencing tensioning protocol. CONCLUSION: Tensioning practices appear to consist of three main approaches, (1) manual tension using a sustained maximum one-handed pull, with tension estimated as 41-60 N, applied near full extension, (2) tensioning device, mean tension of 81.85 N, at 30° knee flexion, (3) individual approach based on size and viscoelastic properties of the graft, patient anthropometry, contralateral comparison to the other knee and age of the patient.

Initial graft tension and the effect on postoperative patient functional outcomes in anterior cruciate ligament reconstruction.

PURPOSE: The aim of this review was to investigate the effect of initial graft tension on patient-specific functional outcomes after anterior cruciate ligament reconstruction and determine whether a particular tension is associated with superior functional outcome. METHODS: We performed a systematic review of prospective randomized trials with a National Health and Medical Research Council Australia level of evidence of III or higher published between 1950 and July 2012. Studies using a semitendinosus-gracilis or bone-patellar tendon-bone autograft that reported graft tension and postoperative functional outcomes were included. Quantitative analysis was performed on available data by calculating effect size (ES) both at various time points and across tensions (in Newtons). RESULTS: Initial search strategies returned 457 original publications, of which 5 articles fulfilled all exclusion and inclusion criteria. The mean score for quality was 5.8 (SD, 1.3), with 12 being the highest possible score. When compared with the preoperative side-to-side difference in anterior tibial displacement, 80 N and 78.9 N of tension recorded the largest effect at 2 weeks or less (ES, -2.98 [range, -3.82 to -2.14]) and 12 months or more (ES, -2.45 [range, -3.40 to -1.51]) postoperatively, respectively. When we compared tensions, the largest effect was toward 80 N when compared with 20 N at 2 weeks or less after surgery (ES, 0.76 [range, 0.17 to 1.35]). CONCLUSIONS: The objective of this review was to systematically assess the literature to determine whether a particular initial graft tension results in superior outcomes after anterior cruciate ligament reconstruction. From the review, there is a trend toward an initial graft tension of 78.5 to 90 N resulting in a reduced side-to-side difference in anterior laxity. However, there is insufficient evidence to conclude whether patient-specific function is improved at any specific tension. LEVEL OF EVIDENCE: Level II, systematic review of Level II studies.

Comparing outcomes of medial parapatellar and subvastus approaches in total knee arthroplasty: a randomized controlled trial.

The medial parapatellar (MP) approach in total knee arthroplasty is more common, but the subvastus (SV) approach is less insulting to the quadriceps. Whether the SV approach affords better outcomes was investigated using 90 participants with knee osteoarthritis, randomized to receive either SV or MP approaches and followed for 18 months. The primary outcome was the American Knee Society Score (AKSS); secondary outcomes included pain, knee range, quadriceps lag, Oxford Knee Score, 3-m timed "Up and Go" test, days to straight leg raise, surgeon perceived difficulty, operation duration, and length of stay. Analysis (n = 76) revealed no significant difference in AKSS (P = .076) or other outcomes, except the following: AKSS Functional scores at 12 and 18 months, favoring the MP (P = .032 and P = .028 respectively); surgeon's perceived difficulty, favoring the MP (P = .001); and days to straight leg raise, favoring the SV (P = .044). This study found that the SV approach offers no clinical benefit over the MP approach.

A comparison of patellar vascularity between the medial parapatellar and subvastus approaches in total knee arthroplasty.

A complication of total knee arthroplasty is patellar avascular necrosis. Surgical approaches for total knee arthroplasty include the medial parapatellar approach (MPa) and, less commonly, the subvastus approach (SVa). The argument that SVa retains better patellar vascularity than the MPa was investigated on 20 participants, (SVa, n = 10; MPa, n = 10) 18 months postoperatively. Outcomes were a radionuclide bone imaging technique, a new bone vascularity scale, and an anterior knee pain numerical assessment scale. Results indicated no significant difference between groups on imaging (P = .935), the components of the bone vascularity scale, or anterior knee pain (P > .999). The SVa appears to offer no benefit over the MPa in terms of patellar vascularity or anterior knee pain.

Systematic review of medial parapatellar and subvastus approaches in total knee arthroplasty.

This systematic review was performed to compare the outcomes of the medial parapatellar and subvastus surgical approaches for total knee arthroplasty. Five studies, published between 1993 and 2001 met the inclusion quality standards for the review. The methodological quality of most studies was poor, and they were not sufficiently homogenous for meta-analysis. We found that the evidence was insufficient to demonstrate a clinical or statistically significant difference between the medial parapatellar and subvastus approaches to total knee arthroplasty across all outcomes. Further trials with robust methodology, objective and functional outcome measures, and follow-up beyond 6 to 12 months are required.

Management of the acute scrotum in a district general hospital: 10-year experience.

The acutely painful scrotum is a common urologic emergency. The primary objective of management is to avoid testicular loss. This requires a high index of clinical suspicion and prompt surgical intervention. In our series conducted between January 1996 and December 2005, 119 patients (age range: 4-62 years) underwent emergency operative exploration for acute scrotal pain. The most common finding was torted cyst of Morgagni (63/119, 52.9%), followed by testicular torsion (41/119, 34.4%). The majority of testicular torsions occurred in the pubertal group (22/41, 53.6%). Only one patient in this group had an unsalvageable testis necessitating orchidectomy, a testicular loss rate in torsion of 2.4%. There were no postoperative wound infections or scrotal haematomas. Testicular salvage depends critically on early surgical intervention, so the delay incurred in diagnostic imaging may extend the period of ischaemia. Furthermore, all radiological investigations have a certain false-negative rate. We advocate immediate surgical exploration of the acute scrotum. We report a low orchidectomy rate (2.4%) in testicular torsion.

Telerehabilitation Feasibility in Total Joint Replacement.

Despite documented benefits, many Total Joint Replacement (TJR) patients find it difficult to access rehabilitation following discharge from hospital. One solution to improve access for TJR patients is telerehabilitation. This study aimed to assess the feasibility of introducing a telerehabilitation program for TJR patients. TJR patients at QEII Jubilee Hospital were invited to complete a questionnaire regarding their access, feelings towards and preferences in using technology. Seventy-five patients were recruited. Most patients had computer access (72%) and internet (69%) at home. Sixty-five percent of participants were willing to participate in telerehabilitation. A significant difference was found between older and younger patients. Watching videos on an electronic device was the preferred method for a technology-based home exercise program and phone call the preferred method of communication. Results indicate telerehabilitation in the TJR population is feasible from the perspective of access to, feelings toward, and preferences for technology.